Transplantation of mesenchymal stem cells to the brain by topical application in an experimental traumatic brain injury model

2013 ◽  
Vol 20 (2) ◽  
pp. 306-309 ◽  
Author(s):  
Ping Kuen Lam ◽  
Anthony Wing Ip Lo ◽  
Kevin Ka Wan Wang ◽  
Henry Chi Ho Lau ◽  
Kevin Kai Chung Leung ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Brain Injury ◽  
Topical Application ◽  
Traumatic Brain Injury Model ◽  
Injury Model ◽  
The Brain ◽  
Experimental Traumatic Brain Injury

scholarly journals Topical application of adipose tissue-derived mesenchymal stem cells (ADMSCs) reduced cerebral edema in experimental traumatic brain injury (TBI)—a preliminary study

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Hui Ma ◽  
Lian Xu Cui ◽  
Ping Kuen Lam ◽  
Cindy S. W. Tong ◽  
Kin K. Y. Lo ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Brain Injury ◽  
Topical Application ◽  
Cerebral Edema ◽  
Therapeutic Effects ◽  
Edema Formation ◽  
Hypoxic Precondition ◽  
Preliminary Study

Abstract Background Our previous studies showed that topical application of mesenchymal stem cells (MSCs) improved functional recovery in rat traumatic brain injury (TBI) model, and hypoxic precondition further enhanced the therapeutic effects of MSCs. There was no previous study on the attenuation of cerebral edema by MSCs. We investigated whether topical application of normoxic and hypoxic MSCs could reduce cerebral edema in an experimental TBI model. Methods Two million normoxic (N = 24) and hypoxic (N = 24) MSCs were applied topically to exposed the cerebral cortex in a controlled cortical impact (CCI) model. The MSCs were fixed in position with fibrin glue. No treatment was given to control animals (TBI only: n = 24). After surgery, four animals in each group were sacrificed daily (day 1 to day 6) for edema evaluation. Normal animals without TBI were used as reference (n = 4). The expressions of GFAP, AQP4, and MMP9 were also investigated by immunofluorescence staining and RT-PCR at day 3. Results The edema peaked within 3 days after TBI. Compared with the control, hypoxic MSCs reduced brain water content significantly (p < 0.05). Both hypoxic and normoxic MSCs downregulated the expression of MMP9 and normalized AQP4 distribution to astrocyte end feet. Conclusion Our preliminary study showed that topical application of hypoxic MSCs suppressed both vasogenic and cytotoxic edema formation.

scholarly journals Transplantation of R-GSIK scaffold with mesenchymal stem cells improves neuroinflammation in a traumatic brain injury model

2020 ◽  
Vol 382 (3) ◽  
pp. 575-583
Author(s):  
Sajad Sahab Negah ◽  
Mohammad Moein Shirzad ◽  
Ghazale Biglari ◽  
Farzin Naseri ◽  
Hassan Hosseini Ravandi ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Brain Injury ◽  
Motor Function ◽  
Inflammatory Responses ◽  
Control Groups ◽  
Self Assembling ◽  
Injury Model ◽  
Recovery Of Motor Function

AbstractNeural tissue engineering has been introduced as a novel therapeutic strategy for traumatic brain injury (TBI). Transplantation of mesenchymal stem cells (MSCs) has been demonstrated to improve functional outcome of brain injury, and RADA4GGSIKVAV (R-GSIK), a self-assembling nano-peptide scaffold, has been suggested to promote the behavior of stem cells. This study was designed to determine the ability of the R-GSIK scaffold in supporting the effects of MSCs on motor function activity and inflammatory responses in an experimental TBI model. A significant recovery of motor function was observed in rats that received MSCs+R-GSIK compared with the control groups. Further analysis showed a reduction in the number of reactive astrocytes and microglial cells in the MSCs and MSCs+R-GSIK groups compared with the control groups. Furthermore, western blot analysis indicated a significant reduction in pro-inflammatory cytokines, such as TLR4, TNF, and IL6, in the MSCs and MSCs+R-GSIK groups compared with the TBI, vehicle, and R-GSIK groups. Overall, this study strengthens the idea that the co-transplantation of MSCs with R-GSIK can increase functional outcomes by preparing a beneficial environment. This improvement may be explained by the immunomodulatory effects of MSCs and the self-assembling nano-scaffold peptide.

scholarly journals Topical application of adipose tissue derived mesenchymal stem cells (ADMSCs) reduced cerebral edema in experimental traumatic brain injury (TBI)- a preliminary study

2020 ◽  
Author(s):  
Hui MA ◽  
Lian Xu Cui ◽  
Ping Kuen Lam ◽  
Cindy SW Tong ◽  
Kin KY Lo ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Brain Injury ◽  
Topical Application ◽  
Cerebral Edema ◽  
Therapeutic Effects ◽  
Edema Formation ◽  
Hypoxic Precondition ◽  
Preliminary Study

Abstract Background: Our previous studies showed that topical application of mesenchymal stem cells (MSCs) improved functional recovery in rat traumatic brain injury (TBI) model, and hypoxic precondition further enhanced the therapeutic effects of MSCs. There was no previous study on the attenuation of cerebral edema by MSCs. Objective: We investigated whether topical application of normoxic and hypoxic MSCs could reduce cerebral edema in an experimental TBI model. Methods: 2 million normoxic (N=24) and hypoxic (N=24) MSCs were applied topically to exposed cerebral cortex in a controlled cortical impact (CCI) model. The MSCs were fixed in position with fibrin glue. No treatment was given to control animals (TBI only: n=24). After surgery, four animals in each group were sacrificed daily (day 1 to day 6) for edema evaluation. Normal animals without TBI were used as reference (n=4). The expressions of GFAP, AQP4 and MMP9 were also investigated by immunofluorescence staining and RT-PCR at day 3. Results: The edema peaked within 3 days after TBI. Compared with the control, hypoxic MSCs reduced brain water content significantly (p<0.05). Both hypoxic and normoxic MSCs downregulated the expression of MMP9 and normalized AQP4 distribution to astrocytes end feet. Conclusion: Our preliminary study showed that topical application of hypoxic MSCs suppressed both vasogenic and cytotoxic edema formation.

scholarly journals Topical application of adipose tissue derived mesenchymal stem cells (ADMSCs) reduced cerebral edema in experimental traumatic brain injury (TBI)- a preliminary study

2020 ◽  
Author(s):  
Hui MA ◽  
Lian Xu Cui ◽  
Ping Kuen Lam ◽  
Cindy SW Tong ◽  
Kin KY Lo ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Brain Injury ◽  
Topical Application ◽  
Cerebral Edema ◽  
Therapeutic Effects ◽  
Edema Formation ◽  
Hypoxic Precondition ◽  
Preliminary Study

Abstract Background: Our previous studies showed that topical application of mesenchymal stem cells (MSCs) improved functional recovery in rat traumatic brain injury (TBI) model, and hypoxic precondition further enhanced the therapeutic effects of MSCs. There was no previous study on the attenuation of cerebral edema by MSCs. Objective: We investigated whether topical application of normoxic and hypoxic MSCs could reduce cerebral edema in an experimental TBI model. Methods: 2 million normoxic (N=24) and hypoxic (N=24) MSCs were applied topically to exposed cerebral cortex in a controlled cortical impact (CCI) model. The MSCs were fixed in position with fibrin glue. No treatment was given to control animals (TBI only: n=24). After surgery, four animals in each group were sacrificed daily (day 1 to day 6) for edema evaluation. Normal animals without TBI were used as reference (n=4). The expressions of GFAP, AQP4 and MMP9 were also investigated by immunofluorescence staining and RT-PCR at day 3. Results: The edema peaked within 3 days after TBI. Compared with the control, hypoxic MSCs reduced brain water content significantly (p<0.05). Both hypoxic and normoxic MSCs downregulated the expression of MMP9 and normalized AQP4 distribution to astrocytes end feet. Conclusion: Our preliminary study showed that topical application of hypoxic MSCs suppressed both vasogenic and cytotoxic edema formation. Keywords: topical, MSCs, cerebral edema, TBI

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