DNA barcoding in concentrated Chinese medicine granules using adaptor ligation-mediated polymerase chain reaction

2018 ◽  
Vol 149 ◽  
pp. 512-516 ◽  
Author(s):  
Yat-Tung Lo ◽  
Pang-Chui Shaw
Keyword(s):  
Polymerase Chain Reaction ◽  
Chinese Medicine ◽  
Dna Barcoding ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Identifikasi Barcode Tumbuhan Gedi Merah (Abelmoschus manihot L. medik) dan Gedi Hijau (Abelmoschus moschatus) Berdasarkan Gen matK

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (2) ◽  
pp. 120
Author(s):  
Yusuf R. Fattah ◽  
Vanda S. Kamu ◽  
Max R. J. Runtuwene ◽  
Lidya I. Momuat
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Barcoding ◽  
In Silico ◽  
Dna Barcode ◽  
In Silico Analysis ◽  
Chain Reaction ◽  
Barcode Dna ◽  
Matk Gene ◽  
Polymerase Chain ◽  
Silico Analysis

Gedi (Abelmoschus L.) merupakan tumbuhan tropis. Tumbuhan ini memilki efek farmakologis. Masyarakat Minahasa mengkonsumsi daun gedi yang direbus tanpa diberi bumbu sebagai obat tradisional untuk menurunkan kadar kolesterol, antihipertensi dan antidiabetes. Suatu metode baru untuk mengidentifikasi dan menganalisis keanekaragaman genetika spesies telah dikembangkan dengan menggunakan potongan gen standar yang dikenal dengan teknik DNA barcoding. Salah satu gen yang terdapat pada tumbuhan yaitu gen matK telah digunakan sebagai gen standar untuk barcoding. Pada penelitian ini telah dilakukan isolasi DNA total dan gen matK penanda barcode DNA dari gedi merah dan gedi hijau, serta analisis in-silico terhadap produk gen matK gedi merah, gedi hijau, dan kerabat terdekatnya. Gen matK diisolasi dan diamplifikasi menggunakan metode Polymerase Chain Reaction (PCR) menggunakan primer forward (5’-CGTACAGTACTTTTGTGTTT ACGAG-3’) dan primer reverse (5’-ACCCAGTCCATCTGGAAATCTTGGTTC-3’). Hasil pengurutan nukleotida DNA barcode matK menunjukkan bahwa sebanyak 828 pb matK berhasil diisolasi untuk tumbuhan gedi merah dan tumbuhan gedi hijau. Urutan nukleotida matK gedi merah dan gedi hijau menunjukkan tingkat kemiripan yang tinggi, yaitu > 95%. Selain itu, hasil analisis in-silico menunjukkan bahwa protein MatK gedi dan kerabat terdekatnya bersifat hidrofobik.Gedi (Abelmoschus L.) is a tropical plant. This plant has the pharmacological effects. Minahasan people consumed boiled gedi without any spices addition to lower cholesterol level, blood pressure, and glucose level. A new method for identifying and analyzing the genetic diversity of species has been developed using standard gene known as DNA barcoding technique. One of the genes found in plants called matK gene was used as standard for DNA barcoding. In this research, identification of DNA barcode of red gedi and green gedi based on matK gene, and in-silico analysis on the matK gene products of red gedi, green gedi, and its closest relatives gedi have been done. matK gene was isolated with Polymerase Chain Reaction (PCR) using forward primer (5'-CGTACAGTACTTTTGTGTTTACGAG-3') and reverse primer (5'-ACCCAGTCCATCTGGAAATCTTGGTTC-3'). Barcode DNA of red and green gedi showed 828 bp nucleotide sequence based on matK gene. In addition, matK of both gedi showed high similarity, i.e. >95%. Furthermore, in-silico analysis of MatK gedi and its closest relative showed that this protein is hidrophobic.

Redescription of Leptus kattikus Haitlinger, 2009 (Actinotrichida, Parasitengona, Erythraeidae) and molecular identification of its host from DNA barcoding

Zootaxa ◽  
2012 ◽  
Vol 3569 (1) ◽  
pp. 67 ◽  
Author(s):  
JOANNA MĄKOL ◽  
MAGDALENA FELSKA ◽  
HANNA MONIUSZKO ◽  
GRZEGORZ ZALEŚNY
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Barcoding ◽  
Molecular Identification ◽  
Host Species ◽  
Chain Reaction ◽  
Alternative Method ◽  
Polymerase Chain ◽  
Host Spectrum ◽  
Host Identification ◽  
Different Levels

A morphology-based redescription of Leptus kattikus Haitlinger, 2009, including re-examination of the holotype and supplemented with data of a series of specimens collected in Vietnam, is provided. Larvae at different levels of engorgement, all separated from one phasmid host, were examined. A polymerase chain reaction, conducted in order to amplify and sequence the DNA of parasitic larvae, resulted in molecular identification of the host species. This represents an alternative method of establishing the host identification for larvae which have already detached from the host prior to examination, and can therefore be employed in further studies of host spectrum.

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

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