La vérification de la PCR-RT pour la détection et la discrimination entre Bordetella pertussis, Bordetella parapertussis et Bordetella holmesii pour le diagnostic de la coqueluche

2020 ◽  
Vol 33 (4) ◽  
pp. 193-197
Author(s):  
M. Elgarini ◽  
A. Hammoumi ◽  
A. Qasmaoui ◽  
Z. Mennane ◽  
M. Benhafid ◽  
...  
Keyword(s):  
Bordetella Pertussis ◽  
Bordetella Parapertussis

scholarly journals Diphtheria-tetanus-pertussis vaccine combined with Bordetella parapertussis vaccine

1962 ◽  
Vol 60 (3) ◽  
pp. 289-293 ◽  
Author(s):  
Neda Köhler-Kubelka
Keyword(s):  
Clinical Examination ◽  
Pertussis Vaccine ◽  
Bordetella Pertussis ◽  
Whooping Cough ◽  
Production Test ◽  
Post Vaccination ◽  
Bordetella Parapertussis ◽  
Cross Reactions ◽  
Combined Vaccine

Investigations carried out to ascertain the ability of various strains of Bordetella pertussis and B. parapertussis to produce agglutinins have shown that the agglutinin response is considerably greater with B. parapertussis.Children inoculated with a combined vaccine in which the parapertussis element contained B. parapertussis in only one-twelfth of the concentration of B. pertussis in the pertussis element showed agglutinins in their sera in titres well above 1:300 for both organisms. There were no cross-reactions and the serological responses were specific throughout. The vaccine used was the standard diphtheria-tetanus-pertussis (DTP) prophylactic to which had been added a vaccine prepared from recently isolated strains of B. parapertussis.Agglutinin titres of both whooping cough components with the combined vaccine were somewhat lower in mice than was the case when monovalent vaccines were used, but they were considered to be satisfactory.It is suggested that the agglutination production test in mice could be used for the assessment of protective power of B. parapertussis vaccines against infection.I wish to thank Dr Ikić, director of the Institute of Immunology, Zagreb, who enabled me to perform all these examinations, further to Dr B. Mravunac and Dr Z. Radanov for having carried out vaccination in children and for the clinical examination of post vaccination reactions.

Etiology of Pertussis Syndrome

PEDIATRICS ◽  
1980 ◽  
Vol 66 (1) ◽  
pp. 50-55
Author(s):  
Margaret A. Keller ◽  
Rouben Aftandelians ◽  
James D. Connor
Keyword(s):  
Bordetella Pertussis ◽  
Bacterial Culture ◽  
Index Case ◽  
Family Group ◽  
Isolation Rate ◽  
Bordetella Parapertussis ◽  
Positive Bacterial Culture ◽  
Serologic Evidence ◽  
Family Contact ◽  
Culture Negative

One hundred patients with clinical pertussis were studied to determine the etiology of pertussis syndrome. Forty-two (42%) of the patients had either Bordetella pertussis or Bordetella parapertussis isolated from the nasopharynx. In an additional 36 (36%) patients, B pertussis was isolated from the nasopharynx of the associated index case or family contact case. Thus, Bordetella was isolated from 78 (78%) of the patients or from their immediate family group. Of the 22 culture-negative patients residing in culture-negative families, 12 had serologic evidence of Bordetella infection and another was from a family group in which two members were seropositive. Therefore, 91 patients (91%) had bacteriologic or serologic evidence of Bordetella infection themselves or within their families. Viral cultures were obtained on 75 of the patients. Adenoviruses were isolated from 33% of those with positive cultures for B pertussis and from 14% of those with negative cultures. In the group without direct or indirect, bacteriologic or serologic evidence of Bordetella infection, the adenoviral isolation rate (13%) was not significantly different from the adenoviral isolation rate (33%) in patients with a positive bacterial culture. These data do not support a role for adenovirus alone in causing pertussis syndrome.

scholarly journals Multicenter Clinical Evaluation of the Automated AriesBordetellaAssay

2018 ◽  
Vol 57 (2) ◽  
Author(s):  
Ryan F. Relich ◽  
Amy Leber ◽  
Stephen Young ◽  
Ted Schutzbank ◽  
Ronald Dunn ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Clinical Evaluation ◽  
Bordetella Pertussis ◽  
Turnaround Time ◽  
False Positives ◽  
Molecular Methods ◽  
Nasopharyngeal Swab ◽  
Limits Of Detection ◽  
Content Type ◽  
Bordetella Parapertussis

ABSTRACTMolecular methods offer superior sensitivity and specificity and reduce testing turnaround time from days to hours for detection ofBordetella pertussisandBordetella parapertussis. In this study, we evaluated the performance of the automated PCR-based AriesBordetellaAssay, which detects bothB. pertussisandB. parapertussisdirectly from nasopharyngeal swab specimens. The limits of detection (LoDs) were 1,800 CFU·ml−1forB. pertussisand 213 CFU·ml−1forB. parapertussis. The assay detected 16/18 uniqueB. pertussis/B. parapertussisstrains. Of 71 potentially cross-reacting organisms, 5 generated false positives in 1/6 replicates; none of 6 additionalBordetellaspp. were erroneously detected. Specimens were stable at 20 to 25°C for at least 10 h, at 4 to 8°C for 10 days, and at temperatures not exceeding −70°C for 6 months. Of 1,052 nasopharyngeal specimens from patients with suspected pertussis, 3.0% (n= 32) wereB. pertussispositive and 0.2% (n= 2) wereB. parapertussispositive. Combining these data with AriesBordetellaAssay data from 57 nasopharyngeal samples with previously confirmedB. pertussisorB. parapertussisdata and with data from 50 contrivedB. parapertussissamples, the proportions of positive and negative agreement of the respective Aries assays with the reference assays were 97.1% and 99.0% forB. pertussisand 100% and 99.7% forB. parapertussis. The AriesBordetellaAssay provides accurate detection and distinction ofB. pertussisandB. parapertussisinfections within 2 h. (This study has been registered at ClinicalTrials.gov under registration no. NCT02862262.)

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