Efficacy of low-dose combinations of diatomaceous earth, spinosad and Trichoderma harzianum for the control of Callosobruchus maculatus and Callosobruchus chinensis on stored cowpea seeds

2021 ◽  
Vol 91 ◽  
pp. 101778
Author(s):  
Hassan A. Gad ◽  
Mohamed S. Al-Anany ◽  
Ayman A.M. Atta ◽  
Samir A.M. Abdelgaleil
2021 ◽  
Author(s):  
Manar Y. Amin ◽  
Abeer Omar Abotaleb ◽  
Refaat A. Mohamed

Abstract ECO2FUME® phosphine fumigant was used to fumigate stored cowpea piles under gas-proof sheets to assess its performance against different developmental stages of Callosobruchus maculatus and Callosobruchus chinensis. The mortality was determined on four developmental stages of C. maculatus and C. chinensis, employing ECO2FUME at different concentrations 25, 30, 40, and 50 g/m3 (2% PH3 and 98% CO2 by weight) for 3-days. All stages of both insect species in packed cowpea stacks were completely controlled at 3-days when applied with an ECO2FUME application rate of 50 g/m3. Cases of pupae of C. maculatus and C. chinensis exhibit the highest resistance than other stages, with 78.2 and 73.93% mortality respectively, at 40 g/m3 after 3-days post-exposure to ECO2FUME. Suppression of F1 generation was obtained after fumigation with the same concentration (50 g/m3). Quality (in terms of cowpea germination) and all chemical constituents of cowpea seeds were non significantly (P ≤ 0.05) affected by the fumigation concentration of 50 g/m3.


2013 ◽  
Vol 31 ◽  
pp. 73-78 ◽  
Author(s):  
Shah Hussain Ahmad Mahdi ◽  
M Khalequzzaman

Effectiveness of diatomaceous earth (DE) and other inert dusts (kaolin powder, paddy husk ash, coal ash, alluvial soil, china clay) and a dust formulation insecticide carbaryl have been tested on the pulse beetles Callosobruchus chinensis L. and C. maculatus (F.). The bioassay of the dusts was done on adult beetles by mixing them with normal food (lentil and black gram seeds). The LD50 of the combined doses of mixtures for C. chinensis have been calculated as 12703.57 and 859.36 ppm for DE+ kaolin powder; 2432.78 and 274.00 ppm for DE+ paddy hush ash; 3430.036 and 426.16 ppm for DE+ coal ash; 12563.47 and 652.29 ppm for DE+ alluvial soil; 2242.81 and 325.76 ppm for DE+ china clay; and 21.33 and 14.45 ppm for DE+ carbaryl after 24 and 48 h after treatment respectively. The LD50 of combined doses of different mixtures for C. maculatus have been calculated as 3640.65 and 503.74 ppm for DE+ kaolin powder; 54946.68 and 987.2394 ppm for DE + paddy hush ash; 61029.04 and 3229.436 ppm for DE+ coal ash; 61029 and 4265.599 ppm for DE+ alluvial soil; 4648.786 and 642.278 ppm for DE+ china clay; and 24.12017 and 15.47023 ppm for DE+ carbaryl after 24 and 48 h after treatment respectively. The co-toxicity coefficient has been calculated and all ratios showed synergistic action. The highest co-toxicity coefficient was recorded as 88885.15 and 92107.22 in DE+ carbaryl at 24 and 48 h after treatment for C. chinensis and 78615.55 and 86004.88 in DE+ carbaryl at 24 and 48 h after treatment for C. maculatus. DOI: http://dx.doi.org/10.3329/ujzru.v31i0.15436Univ. j. zool. Rajshahi Univ. Vol. 31, 2012 pp. 73-78


2021 ◽  
Author(s):  
Manar Y. Amin ◽  
Abeer Omar Abotaleb ◽  
Refaat A. Mohamed

Abstract ECO2FUME gas is an alternative to toxic phosphine fumigant and as a quarantine treatment for the control of a particularly recalcitrant pest, Callosobruchus maculatus and Callosobruchus chinensis. This gas was used to fumigate stored cowpea piles under gas-proof sheets to assess its performance against different developmental stages of Callosobruchus maculatus and Callosobruchus chinensis. The mortality was determined on four developmental stages of C. maculatus and C. chinensis, employing ECO2FUME at different concentrations 25, 30, 40, and 50 g/m3 for 3-days. All stages of both insect species in packed cowpea stacks were completely controlled at 3-days when applied with an ECO2FUME application rate of 50 g/m3. Cases of pupae of C. maculatus and C. chinensis exhibit the highest resistance than other stages, with 78.2 and 73.93% mortality respectively, at 40 g/m3 after 3-days post-exposure to ECO2FUME. Suppression of F1 generation was obtained after fumigation with the same concentration (50 g/m3). Quality (in terms of cowpea germination) and all chemical constituents of cowpea seeds were non significantly (P≤0.05) affected by the fumigation concentration of 50 g/m3.


2021 ◽  
Vol 92 ◽  
pp. 101786
Author(s):  
Hassan A. Gad ◽  
Gomaa F. Abo Laban ◽  
Khaled H. Metwaly ◽  
Fathia S. Al-Anany ◽  
Samir A.M. Abdelgaleil

2020 ◽  
Vol 81 (1) ◽  
Author(s):  
Kayode David Ileke ◽  
Jacobs Mobolade Adesina ◽  
Luke Chinaru Nwosu ◽  
Abimbola Olagunju

Abstract Background Powders and extracts of Piper guineense seeds and leaves were assessed for insecticidal activities against Callosobruchus maculatus in the laboratory at temperature and relative humidity of 29.6 °C and 75.9%, respectively. Bioactive compounds in P. guineense leaves and seeds were also investigated. The powders were tested at rates 1.0, 2.0 and 4.0 g/20 g cowpea seeds while extracts were tested at 1.0, 2.0 and 3.0%. Results Results of contact toxicity assay of the seed powder caused 100% adult mortality at 96 h post-treatment period whereas leaf powder evoked 90% adult mortality within the same period at concentration of 1.0 g/20 g cowpea seeds. Low adult emergence was observed on cowpea seeds treated with 1 g of seed powder with percentage adult emergence of 10.0% and inhibition rate (IR) of 97.5%. Beetle Perforation Index (BPI) obtained from treated cowpea seeds was significantly different (P < 0.05) from BPI of untreated seeds. Extracts of P. guineense seed were more toxic than seed powder. Piper guineense seed extract caused 87.5% adult mortality of C. maculatus while leaf extract caused 70.0% adult mortality within 24 h of infestation at concentration of 1%. Progeny development of C. maculatus was completely inhibited in cowpea treated with 2% and 3% leaf and seed extracts of P. guineense. β-Pinene was the most abundant active compound in P. guineense seed (55.6%) and leaf (48.4%). β-Phellandrene occurred 38.2% in seeds while Ocimene had the least value of 0.2% in seed and 0.5% in leaf. Conclusion The study showed that P. guineense seed powder and extracts were more effective than leaf powder and extract. Utilization of plant products as alternative to synthetic insecticides in protecting cowpea seeds against C. maculatus should be encouraged for enhanced food safety and security. Piper guineense is used as spice and medicine and interestingly safe for human use.


2011 ◽  
Vol 1 (3) ◽  
pp. 168-171
Author(s):  
J. M. Adesina ◽  
L. A. Afolabi ◽  
A. T. Aderibigbe

The efficacy of Senna occidentalis leaf powder against Callosobruchusmaculatus (F) on cowpea treated seeds was evaluated under ambientcondition (32±0.64°C and 68±3% R.H.) at the laboratory of AgriculturalTechnology of Rufus Giwa Polytechnic, Owo. C. maculatus was obtainedfrom Oja‐Oba market, Owo, Ondo State of Nigeria. The insects were rearedand bred in the laboratory. The cowpea seeds used for the bioassay werekept in the freezer for 15 days to eliminate infestation from the field. Theleaf powder was obtained after milling and four dosages of the powder (0.5,1.0, 1.5 and 2.0g) were separately admixed with 20g of cowpea in separatepetri dishes. Ten pairs of C. maculatus were introduced into each petri‐dish.Number of adult mortality, egg laid, percentage egg hatching, adultemergence and weight loss from each treatment were compared withcontrol treatment. It is concluded that the Senna occidentalis contains toxicproperties valuable for the control of C. maculatus.


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