Abstract
The study was conducted to determine effects of three dietary selenium (Se) sources, such as sodium-selenite (S-S), seleno-yeast (S-Y) and seleno-methionine (S-M), on Se concentration, GPX and TXNRD activities, and mRNA expression of 15 representative selenoproteins, and protein expression of four endoplasmic reticulum (ER)-resided selenoproteins in a wide range of tissues of yellow catfish. Compared with S-S and S-M group, dietary S-Y significantly decreased growth performance and feed utilization of yellow catfish. Dietary Se sources significantly influenced Se contents in the spleen, dorsal muscle and the kidney, GPX activities in spleen, kidney, intestine, muscle and mesenteric fat, and TXNRD activities in the heart, intestine and mesenteric fat. Among 10 tested tissues, dietary Se sources influenced mRNA expression of GPX4 and SELENOK in 3 tissues, GPX3, SELENOS and TXNRD2 in 4 tissues, SELENOF, SELENON and DIO2 in 5 tissues, SELENOM, GPX1/2 and TXNRD3 in 6 tissues, SELENOW in 7 tissue, SELENOP and SELENOT in 8 tissues. Based on these observations above, sodium-selenite and seleno-methionine seem to be suitable Se sources for improving growth performance and feed utilization of yellow catfish. Dietary Se sources differentially influence the expression of selenoproteins in various tissues of yellow catfish. For the first time, we determined the expression of selenoproteins in fish in responses to dietary Se sources, which contributes to a better understanding of the functions and regulatory mechanisms of selenoporteins.
Molecular characterization and expression analysis of a novel C-type lectin (CTL) gene in yellow catfish Pelteobagrus fulvidraco
