scholarly journals A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates

2020 ◽  
Vol 32 (1) ◽  
pp. 112-117
Author(s):  
Silvina S. Maidana ◽  
Samuel Miño ◽  
Romina M. Apostolo ◽  
Gabriel A. De Stefano ◽  
Sonia A. Romera
Keyword(s):  
Multiplex Pcr ◽  
Rapid Test ◽  
Bovine Herpesvirus ◽  
Restriction Endonuclease Analysis ◽  
Snp Analysis ◽  
Hindiii Site ◽  
Bovine Herpesvirus 1 ◽  
Clinical Syndromes ◽  
Herpesvirus 1

Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need simpler and cheaper screening assays for routine testing. We designed a standard multiplex PCR followed by a REA assay allowing straightforward subclassification of all BoHV-1 isolates tested into 1.1, 1.2a, and 1.2b subtypes based on the analysis of fragment length polymorphism. Our standard multiplex PCR-REA was used to analyze 33 field strains of BoHV-1 isolated from various tissues. The assay confirmed the subtype identified previously by REA. In addition, non-polymorphic or undigested fragments were sequenced in order to confirm the mutation affecting the RE HindIII site. Our PCR-REA method is an affordable and rapid test that will subtype all BoHV-1 strains.

scholarly journals Identification and characterization of BICP27, an early protein of bovine herpesvirus 1 which may stimulate mRNA 3' processing

1996 ◽  
Vol 77 (4) ◽  
pp. 615-625 ◽  
Author(s):  
M. Singh ◽  
C. Fraefel ◽  
L. J. Bello ◽  
W. C. Lawrence ◽  
M. Schwyzer
Keyword(s):  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Early Protein ◽  
Herpesvirus 1 ◽  
Identification And Characterization

Characterization of anti-idiotype reagents to bovine herpesvirus-1 monoclonal antibody

1988 ◽  
Vol 20 (1) ◽  
pp. 1-14 ◽  
Author(s):  
D.J. Orten ◽  
F. Blecha ◽  
J.L. Morrill ◽  
H.C. Minocha
Keyword(s):  
Monoclonal Antibody ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1

Phylogenetic analysis and characterization of bovine herpesvirus-1 in cattle of China, 2016–2019

2020 ◽  
Vol 85 ◽  
pp. 104416 ◽  
Author(s):  
Yulong Zhou ◽  
Xuyang Li ◽  
Yachao Ren ◽  
Xilin Hou ◽  
Yu Liu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1

scholarly journals Characterization of the nuclear and nucleolar localization signals of bovine herpesvirus-1 infected cell protein 27

2009 ◽  
Vol 145 (2) ◽  
pp. 312-320 ◽  
Author(s):  
Hong Guo ◽  
Qiong Ding ◽  
Fusen Lin ◽  
Weiwei Pan ◽  
Jianyin Lin ◽  
...  
Keyword(s):  
Infected Cell ◽  
Bovine Herpesvirus ◽  
Cell Protein ◽  
Nucleolar Localization ◽  
Bovine Herpesvirus 1 ◽  
Infected Cell Protein ◽  
Herpesvirus 1 ◽  
Infected Cell Protein 27

scholarly journals Identification and Characterization of a Bovine Herpesvirus-1 (BHV-1) Glycoprotein gL Which Is Required for Proper Antigenicity, Processing, and Transport of BHV-1 Glycoprotein gH

Virology ◽  
1996 ◽  
Vol 219 (1) ◽  
pp. 66-76 ◽  
Author(s):  
SUNIL K. KHATTAR ◽  
SYLVIA VAN DRUNEN LITTEL-VAN DEN HURK ◽  
SAM K. ATTAH-POKU ◽  
LORNE.A. BABIUK ◽  
SURESH K. TIKOO
Keyword(s):  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1 ◽  
Identification And Characterization

Production and characterization of bovine herpesvirus 1 glycoprotein B ectodomain derivatives in an hsp70A gene promoter-based expression system

1996 ◽  
Vol 141 (10) ◽  
pp. 2019-2029 ◽  
Author(s):  
Y. Li ◽  
S. Van Drunen Littel-Van den Hurk ◽  
X. Liang ◽  
L. A. Babiuk
Keyword(s):  
Expression System ◽  
Gene Promoter ◽  
Bovine Herpesvirus ◽  
Glycoprotein B ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1

scholarly journals First Report on the Detection and Molecular Characterization of Bovine Herpesvirus 1 from a Clinical case of Infectious Bovine Rhinotracheitis in Pakistan

2021 ◽  
Vol 41 (01) ◽  
pp. 160-162
Author(s):  
Aayesha Riaz
Keyword(s):  
Molecular Characterization ◽  
Nested Pcr ◽  
Bovine Herpesvirus ◽  
Nasal Discharge ◽  
Infectious Bovine Rhinotracheitis ◽  
Bovine Herpesvirus 1 ◽  
Nasal Swabs ◽  
Herpesvirus 1 ◽  
First Time

Bovine herpesvirus 1 (BHV-1) is a noteworthy reason for many Cattle/Buffalo diseases. Infectious bovine rhinotracheitis (IBR) is one of the diseases which are caused by the BHV-1. In the present study a cow which was suspected of IBR was examined. The animal was suffering from fever and respiratory distress along with rhinitis (red nose), in appetence, and dyspnea. The nasal mucosa and muzzle were distinctly inflamed, with nasal discharge. DNA from blood samples and nasal swabs were subjected to nested PCR using glycoprotein B gene (gB) Primers. The samples were found positive for BHV-1 gB gene. Sequencing and phylogenetic analysis revealed close similarities with other BHV-1 gB gene sequences. The accession numbers assigned to this pioneer sequences in GenBank are MT449510 (BHV-1-Pak 1) and MT449511 (BHV-1-Pak 2). In this study, we reported for the first time the detection of DNA of BHV-1 through nested PCR assay and molecular characterization of BHV-1 gB gene in Pakistan. This study will be useful in further diagnoses of BHV-1 in Pakistan and in development of BHV-1 vaccine to reduce economical losses due to IBR

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