scholarly journals Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus

2016 ◽  
Vol 234 ◽  
pp. 34-42 ◽  
Author(s):  
Jianqiang Zhang ◽  
Yun-Long Tsai ◽  
Pei-Yu Alison Lee ◽  
Qi Chen ◽  
Yan Zhang ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Porcine Epidemic Diarrhea Virus ◽  
Rt Pcr ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea ◽  
Insulated Isothermal Pcr ◽  
Pcr Test

scholarly journals One-step triplex reverse-transcription PCR detection of porcine epidemic diarrhea virus, porcine sapelovirus, and porcine sapovirus

2019 ◽  
Vol 31 (6) ◽  
pp. 909-912 ◽  
Author(s):  
Chunyan Jiang ◽  
Haijian He ◽  
Chaoying Zhang ◽  
Xiaoju Zhang ◽  
Jianfeng Han ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Porcine Epidemic Diarrhea Virus ◽  
Detection Method ◽  
Rt Pcr ◽  
Diarrhea Virus ◽  
Reverse Transcription Pcr ◽  
Porcine Sapovirus ◽  
One Step ◽  
Porcine Epidemic Diarrhea ◽  
Porcine Sapelovirus

Swine diarrhea can be caused by multiple agents, including porcine epidemic diarrhea virus (PEDV), porcine sapelovirus (PSV), and porcine sapovirus (SaV). We designed a one-step triplex reverse-transcription PCR (RT-PCR) detection method including 3 pairs of primers that focused on the S1 gene of PEDV, a conserved gene of PSV, and the VP1 gene of SaV. The optimal concentrations of upstream and downstream primers in the triplex RT-PCR were 0.24 μM for PEDV, 0.15 μM for PSV, and 0.2 μM for SaV, and the optimal annealing temperature was 55.5°C. Triplex RT-PCR assessment of 402 piglet diarrhea samples was compared with conventional individual RT-PCR. Concordance rates in both tests for individual viruses were 100%, 97.6%, and 94.4% for PEDV, PSV, and SaV, respectively. PEDV, PSV, and SaV were detected in 57.2%, 10.4%, and 9.0% of the samples, respectively. The high sensitivity and specificity of this triplex RT-PCR–based detection method for PEDV, PSV, and SaV could allow rapid detection and analysis of mixed infections by these 3 viruses.

Direct and rapid detection of porcine epidemic diarrhea virus by RT-PCR

1997 ◽  
Vol 69 (1-2) ◽  
pp. 191-195 ◽  
Author(s):  
Kiyoyasu Ishikawa ◽  
Hideto Sekiguchi ◽  
Tomoe Ogino ◽  
Shoko Suzuki
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Rapid Detection ◽  
Rt Pcr ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea
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