A direct high-throughput in Cell-ELISA for measuring infectivity of cytopathic and non-cytopathic bovine viral diarrhoea virus strains applied to the assessment of antiviral activity

Background: A number of compounds were examined for their inhibitory effect on bovine viral diarrhoea virus (BVDV) replication in cell cultures and found that some cyclooxygenase (COX) inhibitors had antiviral activity against the virus. Methods: Determination of compounds for their anti-BVDV activity was on the basis of the inhibition of virus-induced cytopathogenicity in Mardin–Darby bovine kidney (MDBK) cells. Anti-hepatitis C virus (HCV) activity was assessed by the inhibition of viral RNA synthesis in the subgenomic HCV RNA replicon cells. Results: Among the test compounds, 5-(4-chlorophenyl)-1-(4-methoxyphenyl)-3-(trifluoromethyl)-1 H-pyrazole (SC-560) was the most active against BVDV, and its 50% effective and cytotoxic concentrations were 10.9 ±2.8 and 93.9 ±24.5 μM in virus and mock-infected MDBK cells, respectively. The compound also suppressed BVDV RNA synthesis in a dose-dependent fashion. Studies on the mechanism of action revealed that SC-560 did not interfere with viral entry to the host cells. Furthermore, it was assumed that the antiviral activity of SC-560 was not associated with its inhibitory effect on COX. The combination of SC-560 and interferon-α was additive to synergistic in inhibiting BVDV replication. More importantly, the compound proved to be a selective inhibitor of HCV replication. Conclusions: SC-560 and its derivative might have potential as novel antiviral agents against HCV.

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Pathogenicity of a bovine viral diarrhoea virus strain in pregnant sows: Short communication

Acta Veterinaria Hungarica ◽

10.1556/004.49.2001.1.14 ◽

2001 ◽

Vol 49 (1) ◽

pp. 117-120 ◽

Cited By ~ 4

Author(s):

G. Kulcsár ◽

P. Soós ◽

L. Kucsera ◽

R. Glávits ◽

V. Pálfi

Keyword(s):

Virus Strain ◽

Clinical Symptoms ◽

Classical Swine Fever ◽

Bovine Viral Diarrhoea Virus ◽

Biological Properties ◽

Asymptomatic Infection ◽

Bovine Viral Diarrhoea ◽

Diarrhoea Virus ◽

The Given ◽

Virus Strains

The biological properties of bovine viral diarrhoea virus (BVDV) strain Oregon C24V were studied after intranasal and subcutaneous infection of pregnant sows. This virus strain is widely used in Hungary for immunising cattle against bovine viral diarrhoea (BVD). Based upon the results of the clinical, gross pathological, histopathological and virological examinations it can be established that the given strain caused asymptomatic infection and serological conversion in sows that were in the second third of gestation. The virus caused clinically apparent disease in some of the piglets born at term, which indicates that it had crossed the placenta. More than half (57%) of the live-born piglets died within 60 days of birth. The sows and their progeny did not shed the virus. BVDV infection has great differential diagnostic importance in pigs, as classical swine fever (CSF) virus strains of reduced virulence cause similar clinical symptoms and gross and histopathological changes.

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Antiviral Activity of Geneticin against Bovine Viral Diarrhoea Virus

Antiviral Chemistry and Chemotherapy ◽

10.1177/095632020801900105 ◽

2008 ◽

Vol 19 (1) ◽

pp. 33-40 ◽

Cited By ~ 8

Author(s):

Alexander V Birk ◽

Edward J Dubovi ◽

Xianchao Zhang ◽

Hazel H Szeto

Keyword(s):

Antiviral Activity ◽

Bovine Viral Diarrhoea Virus ◽

Bovine Viral Diarrhoea ◽

Diarrhoea Virus

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Preliminary serological characterization of bovine viral diarrhoea virus strains using monoclonal antibodies

Veterinary Microbiology ◽

10.1016/0378-1135(86)90048-9 ◽

1986 ◽

Vol 12 (3) ◽

pp. 195-200 ◽

Cited By ~ 64

Author(s):

W. Peters ◽

I. Greiser-Wilke ◽

V. Moennig ◽

B. Liess

Keyword(s):

Monoclonal Antibodies ◽

Bovine Viral Diarrhoea Virus ◽

Bovine Viral Diarrhoea ◽

Serological Characterization ◽

Diarrhoea Virus ◽

Virus Strains

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Attempt to Discriminate between Bovine Viral-diarrhoea Virus Strains using Polymerase Chain Reaction

Journal of Veterinary Medicine Series B ◽

10.1111/j.1439-0450.1995.tb00709.x ◽

1995 ◽

Vol 42 (1-10) ◽

pp. 257-265 ◽

Cited By ~ 7

Author(s):

M. Tajima ◽

R. Kirisawa ◽

M. Taguchi ◽

H. Iwai ◽

Y. Kawakami ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Bovine Viral Diarrhoea Virus ◽

Bovine Viral Diarrhoea ◽

Chain Reaction ◽

Diarrhoea Virus ◽

Polymerase Chain ◽

Virus Strains

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Synthesis and Antiviral Activity of New Thiazole, 1,2,4-Triazol and Oxindole Derivatives

Chemistry Journal of Moldova ◽

10.19261/cjm.2011.06(1).03 ◽

2011 ◽

Vol 6 (1) ◽

pp. 101-109

Author(s):

Oleg Radul ◽

Natalia Sucman ◽

Serghei Pogrebnoi ◽

Alic Barba ◽

Athina Geronikaki ◽

...

Keyword(s):

Antiviral Activity ◽

Cell Lines ◽

Bovine Viral Diarrhoea Virus ◽

Moderate Activity ◽

Bovine Viral Diarrhoea ◽

Reference Compound ◽

Activity Evaluation ◽

Diarrhoea Virus ◽

Derivatives Of ◽

Hiv 1

The synthesis and antiviral activity evaluation of new derivatives of 2-aminothiazole, 1,2,4-triazole, as well as oxindoles has been realized. The synthesized compounds exhibited different cytotoxicity, in particular, oxindols 4, 5 , 7 , 8 , 9, 10, 11, 12, 13, 58 as well as thiazole/triazole 73 and 75 turned out to be the most cytotoxic for MT-4 cell lines. The compounds 11, 12, 73, and 75 are more toxic than reference compound Efavirenz. As far as the antiviral activity is concerned, none of the title compounds turned out active against Reo-1, Sb-1, VSV, RSV, YFV and VV viruses. The results obtained against Bovine Viral Diarrhoea Virus (BVDV) showed that nine compounds (six from oxindol’s seria 6, 12, 13, 52, 56, 58 and three 73, 75, 77 of triazole homologues) resulted moderate active. Among all of them, the most potent compound was 52, with EC50 of 6.6 μM. Studies of effect of synthesized compounds against Coxsakie Virus (CVB-2) revealed that only two compounds, 13 and 73 exhibit moderate activity (EC50 >40 and >18 μM, respectively). It should be noticed that eleven compounds, 4, 5, 7, 8, 9, 10, 11, 12, 13, 58, and 75 showed moderate activity against HIV-1 (EC50 >16 – m >59μM).

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