A Comparative study of direct fluorescent antibody, mouse inoculation, and tissue culture infection testing for Rabies diagnoses

2021 ◽  
pp. 114426
Author(s):  
A.C. Rodrigues ◽  
R.M.N. Marcusso ◽  
D.N. Souza ◽  
W.O. Fahl ◽  
G.M.M. Caporale ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Comparative Study ◽  
Fluorescent Antibody ◽  
Direct Fluorescent Antibody

scholarly journals Performance of the Syva Direct Fluorescent Antibody Assay for Chlamydia in a Low-Prevalence Population

1993 ◽  
Vol 1 (1) ◽  
pp. 2-6 ◽  
Author(s):  
Mark B. Reedy ◽  
Patricia J. Sulak ◽  
William B. McCombs III ◽  
Thomas J. Kuehl
Keyword(s):  
Tissue Culture ◽  
Fluorescent Antibody ◽  
Transmitted Disease ◽  
The United States ◽  
Current Data ◽  
Chlamydia Infection ◽  
Culture Methods ◽  
Predictive Values ◽  
Direct Fluorescent Antibody ◽  
Low Prevalence

Chlamydia trachomatisis the most common reportable sexually transmitted disease (STD) in the United States. In the 1980s, rapid diagnostic tests for chlamydia began to replace more cumbersome tissue culture methods. Current data on rapid antigen detection assays demonstrate acceptable sensitivity, specificity, and predictive values in populations with a high prevalence of chlamydia. Few studies report the performance of these assays in a low-prevalence obstetric and gynecologic (Ob/Gyn) population, This study compares the most commonly used direct fluorescent antibody (DFA) assay (Syva Microtrak) with tissue culture (TC) in a low-prevalence population. Endocervical specimens (775) were tested from women at risk for chlamydia infection, and the prevalence was found to be 7.7%. The DFA assay demonstrated a sensitivity of 80% and a specificity of 97% compared with TC. The positive and negative predictive values were 72% and 98%, respectively. The results of this study indicate that the Syva DFA assay lacks the sensitivity and positive predictive value for routine use in Ob/Gyn populations with a lowprevalence ofC. trachomatis.

scholarly journals Mycoplasmas in cell cultures from rheumatoid synovial membranes

1971 ◽  
Vol 69 (1) ◽  
pp. 17-25 ◽  
Author(s):  
K. B. Fraser ◽  
P. V. Shirodaria ◽  
Margaret Haire ◽  
D. Middleton
Keyword(s):  
Rheumatoid Arthritis ◽  
Tissue Culture ◽  
Fluorescent Antibody ◽  
Fluorescent Staining ◽  
Antibody Staining ◽  
Synovial Fluids ◽  
Direct Fluorescent Antibody ◽  
The Common ◽  
Synovial Membranes ◽  
Fluorescent Antibody Staining

SUMMARYTen strains of myocoplasmas were recovered from cultures of synovium or cultures inoculated with synovial fragments from rheumatoid arthritis and one from osteo-arthritis. The source of the organisms is not known. Patients with rheumatoid arthritis had no complement-fixing antibody and no fluorescent staining antibody against the mycoplasmas isolated and no mycoplasma antigen was detected by immunofluorescence in sections of synovia and in synovial fluids.The strains isolated were of two main serological types and could be distinguished by direct fluorescent antibody staining from standard types of human commensals and the common tissue-culture contaminants. One may beMycoplasma laidlawii.

A Most Probable Number Method for the Enumeration of Legionella Bacteria in Water

1991 ◽  
Vol 24 (2) ◽  
pp. 143-147 ◽  
Author(s):  
N. A. Grabow ◽  
R. Kfir ◽  
W. O. K. Grabow
Keyword(s):  
Water Samples ◽  
Membrane Filtration ◽  
Quantitative Method ◽  
Fluorescent Antibody ◽  
Most Probable Number ◽  
Probable Number ◽  
Comparative Tests ◽  
Antibody Staining ◽  
Direct Fluorescent Antibody ◽  
Yeast Extract Agar

A new quantitative method for the enumeration of Legionella bacteria in water is described. Appropriate tenfold serial dilutions of water samples concentrated by membrane filtration are plated in triplicate on buffered charcoal yeast extract agar. After incubation for 3 days representative smears from individual plates are tested for the presence of Legionella by direct fluorescent antibody staining. The number of positive plates in each dilution is used to calculate the Legionella count by means of conventional most probable number statistics. In comparative tests on a variety of water samples this method yielded significantly higher counts than previously used procedures.

Fluorescent Antibody and Complement-Fixation Tests of Agents Isolated In Tissue Culture from Measles Patients.

1955 ◽  
Vol 90 (1) ◽  
pp. 118-122 ◽  
Author(s):  
S. M. Cohen ◽  
I. Gordon ◽  
F. Rapp ◽  
J. C. Macaulay ◽  
S. M. Buckley
Keyword(s):  
Tissue Culture ◽  
Complement Fixation ◽  
Fluorescent Antibody
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