Fluorescent Antibody and Complement-Fixation Tests of Agents Isolated In Tissue Culture from Measles Patients.

1955 ◽  
Vol 90 (1) ◽  
pp. 118-122 ◽  
Author(s):  
S. M. Cohen ◽  
I. Gordon ◽  
F. Rapp ◽  
J. C. Macaulay ◽  
S. M. Buckley
Keyword(s):  
Tissue Culture ◽  
Complement Fixation ◽  
Fluorescent Antibody

scholarly journals A COMPLEMENT FIXATION TEST FOR POLIOMYELITIS

1955 ◽  
Vol 102 (2) ◽  
pp. 133-150 ◽  
Author(s):  
Nathalie J. Schmidt ◽  
Edwin H. Lennette ◽  
Keyword(s):  
Tissue Culture ◽  
Antibody Response ◽  
Clinical Diagnosis ◽  
Antibody Titer ◽  
Virus Type ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Laboratory Diagnosis ◽  
Fixation Test ◽  
Dual Infections

A macroscopic (tube) complement fixation test for poliomyelitis, using infected tissue culture fluids, is described. The test was applied to 27 individuals with a clinical diagnosis of poliomyelitis. In 18 patients it was possible to make a laboratory diagnosis of poliomyelitis on the basis of a rise in complement-fixing antibody titer and in 4 others on the basis of a high stationary antibody titer. One individual gave a high and equal antibody response to two virus types, 3 others had no detectable antibody, and 1 appeared not to have poliomyelitis. Heterotypic reactions were encountered, but gave little difficulty in interpreting homologous responses. In those patients from whom a virus had been recovered, the serologic findings corresponded to the virus type recovered. The possible occurrence of dual infections with the viruses of poliomyelitis and Western equine and St. Louis encephalitis is discussed.

scholarly journals SEQUENTIAL CELLULAR CHANGES PRODUCED BY TYPES 5 AND 7 ADENOVIRUSES IN HELA CELLS AND IN HUMAN AMNIOTIC CELLS

1959 ◽  
Vol 110 (5) ◽  
pp. 827-844 ◽  
Author(s):  
Georgiana S. Boyer ◽  
Floyd W. Denny ◽  
Harold S. Ginsberg
Keyword(s):  
Tissue Culture ◽  
Cell Nucleus ◽  
Hela Cells ◽  
Fluorescent Antibody ◽  
Intranuclear Inclusions ◽  
Culture Cells ◽  
Cellular Changes ◽  
Tissue Culture Cells ◽  
Cytological Changes ◽  
Amniotic Cells

The sequential cytological changes which develop in tissue culture cells infected with adenovirus types 5 and 7 are described and compared with those produced by adenovirus types 1, 2, 3, and 4. The evidence that is presented indicates that types 1, 2, and 5 belong to one major subdivision of the adenovirus group and types 3, 4, and 7 to another. That the host cell nucleus is the principal site of adenovirus synthesis has been confirmed by fluorescent antibody studies. They have demonstrated the occurrence of type-specific adenovirus antigen in the characteristic intranuclear inclusions and other virus-induced structures reported to contain virus-like particles or shown by electronmicroscopy.

scholarly journals Serologic evidence of the postoperative persistence of hydatid cysts in man

1975 ◽  
Vol 75 (3) ◽  
pp. 333-340 ◽  
Author(s):  
R. M. Matossian ◽  
G. F. Araj
Keyword(s):  
Prognostic Value ◽  
Complement Fixation ◽  
Recurrent Disease ◽  
Fluorescent Antibody ◽  
Hydatid Cysts ◽  
Presumptive Diagnosis ◽  
Pulmonary Cysts ◽  
Serologic Evidence ◽  
Antibody Tests ◽  
Postoperative Study

SUMMARYThe sensitivity of the haemagglutination, complement-fixation and indirect fluorescent antibody tests in the diagnosis of primary and recurrent hydatid disease is presented. In the first, diagnostic titres were obtained in 90%, 80% and 96% of 50 patients with hepatic and 64%, 60% and 68% of 25 patients with pulmonary cysts, respectively. In the postoperative study of 62 patients, elevated titres of HA, CF and IFA antibodies in 30 led to the presumptive diagnosis of recurrent disease, confirmed later by radiological and surgical follow-ups. In the 32 others the proportion of positive reactors was 29%, 6% and 28% in HA, CF and IFA tests. These patients had no detectable cysts. This indicates that in post-operative patients a negative CF test may be of a better prognostic value in indicating absence of cysts than the HA and IFA tests.

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