scholarly journals PS206. “Back-Table” Manipulation of Human Saphenous Vein Significantly Impairs Endothelial and Smooth Muscle Function

2011 ◽  
Vol 53 (6) ◽  
pp. 83S
Author(s):  
Michael J. Osgood ◽  
Kyle M. Hocking ◽  
Kevin W. Sexton ◽  
Padmini Komalavilas ◽  
Joyce Cheung-Flynn ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Saphenous Vein ◽  
Muscle Function ◽  
Human Saphenous Vein ◽  
Smooth Muscle Function

N-Acetylcysteine Does Not Improve the Endothelial and Smooth Muscle Function in the Human Saphenous Vein

2007 ◽  
Vol 41 (3) ◽  
pp. 239-245 ◽  
Author(s):  
Muhammad Anees Sharif ◽  
Ulvi Bayraktutan ◽  
Ian Stuart Young ◽  
Chee Voon Soong
Keyword(s):  
Smooth Muscle ◽  
Saphenous Vein ◽  
Muscle Function ◽  
Ex Vivo ◽  
Smooth Muscle Relaxation ◽  
Organ Bath ◽  
Human Saphenous Vein ◽  
Vein Segment ◽  
Significant Difference ◽  
Smooth Muscle Function

Oxidative stress can lead to vein graft dysfunction in the saphenous vein. This ex vivo study is aimed to compare the effects of increasing concentrations of the antioxidant N-acetylcysteine (NAC) with heparinized saline (HS) on endothelial and smooth muscle function in the human saphenous vein. Long saphenous vein segment obtained during infrainguinal bypass surgery was divided into 7 rings; 1 immersed in HS and the remaining 6 in increasing NAC concentrations (0.0025%, 0.005%, 0.01%, 0.02%, 0.03%, and 0.04%). Rings were mounted in an organ bath, and relaxant responses to acetylcholine and sodium nitroprusside were assessed through isometric tension studies. Endothelium-dependent relaxations were observed in 77 vein segments from 11 patients. No significant difference was seen in veins treated with either lower NAC concentrations (0.0025%, 0.005%, 0.01%, 0.02%, and 0.03%) or HS. However, HS-treated veins showed significantly better relaxation compared to those treated with maximum (0.04%) NAC ( P < .05). Endothelium-independent relaxations were observed in 91 segments from 13 patients. No difference in relaxation was observed between veins treated with HS or any of the NAC concentrations. In conclusion, lower NAC concentrations do not offer better endothelial protection than HS, whereas the highest NAC concentration has a detrimental effect on endothelium-dependent relaxation. Moreover, NAC did not show beneficial effect on direct smooth muscle relaxation.

Cryopreservation affects endothelial and smooth muscle function of canine autogenous saphenous vein grafts

1991 ◽  
Vol 13 (5) ◽  
pp. 584-592 ◽  
Author(s):  
Jonathan B. Towne ◽  
James R. Elmore ◽  
Peter R. Gloviczki ◽  
Kelvin G. M. Brockbank
Keyword(s):  
Smooth Muscle ◽  
Saphenous Vein ◽  
Muscle Function ◽  
Vein Grafts ◽  
Saphenous Vein Grafts ◽  
Smooth Muscle Function

scholarly journals Cryopreservation affects endothelial and smooth muscle function of canine autogenous saphenous vein grafts

1991 ◽  
Vol 13 (5) ◽  
pp. 584-592 ◽  
Author(s):  
James R. Elmore ◽  
Peter Gloviczki ◽  
Kelvin G.M. Brockbank ◽  
Virginia M. Miller
Keyword(s):  
Smooth Muscle ◽  
Saphenous Vein ◽  
Muscle Function ◽  
Vein Grafts ◽  
Saphenous Vein Grafts ◽  
Smooth Muscle Function

Minimally invasive saphenous vein harvesting: effects on endothelial and smooth muscle function

2001 ◽  
Vol 71 (5) ◽  
pp. 1503-1507 ◽  
Author(s):  
Edward A. Black ◽  
Tomasz J. Guzik ◽  
Nick E.J. West ◽  
Karen Campbell ◽  
Ravi Pillai ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Minimally Invasive ◽  
Saphenous Vein ◽  
Muscle Function ◽  
Smooth Muscle Function ◽  
Vein Harvesting

SMALL BOWEL ISCHEMIA/REPERFUSION (II/R) ALTERS COLONIC SMOOTH MUSCLE FUNCTION.

Shock ◽  
1998 ◽  
Vol 9 (Supplement) ◽  
pp. 13
Author(s):  
DT Dempsey ◽  
BS Myers ◽  
JP Ryan ◽  
J Carroll ◽  
SI Myers
Keyword(s):  
Smooth Muscle ◽  
Small Bowel ◽  
Muscle Function ◽  
Ischemia Reperfusion ◽  
Bowel Ischemia ◽  
Smooth Muscle Function

Effect of Flush-Perfusion on Vascular Endothelial and Smooth Muscle Function

1997 ◽  
Vol 64 (4) ◽  
pp. 1075-1081 ◽  
Author(s):  
Richard Ingemansson ◽  
Algimantas Budrikis ◽  
Ramunas Bolys ◽  
Trygve Sjöberg ◽  
Stig Steen
Keyword(s):  
Smooth Muscle ◽  
Muscle Function ◽  
Vascular Endothelial ◽  
Smooth Muscle Function

scholarly journals Effects of Scleroderma Antibodies and Pooled Human Immunoglobulin on Anal Sphincter and Colonic Smooth Muscle Function

2012 ◽  
Vol 143 (5) ◽  
pp. 1308-1318 ◽  
Author(s):  
Jagmohan Singh ◽  
Sidney Cohen ◽  
Vaibhav Mehendiratta ◽  
Fabian Mendoza ◽  
Sergio A. Jimenez ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Anal Sphincter ◽  
Muscle Function ◽  
Human Immunoglobulin ◽  
Smooth Muscle Function

scholarly journals Cross-talk between angiotensin II and IGF-1-induced connexin 43 expression in human saphenous vein smooth muscle cells

2011 ◽  
Vol 15 (8) ◽  
pp. 1695-1702 ◽  
Author(s):  
Guanghong Jia ◽  
Anshu Aggarwal ◽  
Amanuel Yohannes ◽  
Deepak M. Gangahar ◽  
Devendra K. Agrawal
Keyword(s):  
Smooth Muscle ◽  
Angiotensin Ii ◽  
Smooth Muscle Cells ◽  
Cross Talk ◽  
Saphenous Vein ◽  
Connexin 43 ◽  
Muscle Cells ◽  
Human Saphenous Vein

scholarly journals Fundamental Roles of Axial Stretch in Isometric and Isobaric Evaluations of Vascular Contractility

2019 ◽  
Vol 141 (3) ◽  
Author(s):  
Alexander W. Caulk ◽  
Jay D. Humphrey ◽  
Sae-Il Murtada
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Muscle Function ◽  
Contractile Function ◽  
Contractile Activity ◽  
Comparison Of Methods ◽  
Axial Stretch ◽  
Smooth Muscle Function ◽  
In Vivo Loading

Vascular smooth muscle cells (VSMCs) can regulate arterial mechanics via contractile activity in response to changing mechanical and chemical signals. Contractility is traditionally evaluated via uniaxial isometric testing of isolated rings despite the in vivo environment being very different. Most blood vessels maintain a locally preferred value of in vivo axial stretch while subjected to changes in distending pressure, but both of these phenomena are obscured in uniaxial isometric testing. Few studies have rigorously analyzed the role of in vivo loading conditions in smooth muscle function. Thus, we evaluated effects of uniaxial versus biaxial deformations on smooth muscle contractility by stimulating two regions of the mouse aorta with different vasoconstrictors using one of three testing protocols: (i) uniaxial isometric testing, (ii) biaxial isometric testing, and (iii) axially isometric plus isobaric testing. Comparison of methods (i) and (ii) revealed increased sensitivity and contractile capacity to potassium chloride and phenylephrine (PE) with biaxial isometric testing, and comparison of methods (ii) and (iii) revealed a further increase in contractile capacity with isometric plus isobaric testing. Importantly, regional differences in estimated in vivo axial stretch suggest locally distinct optimal biaxial configurations for achieving maximal smooth muscle contraction, which can only be revealed with biaxial testing. Such differences highlight the importance of considering in vivo loading and geometric configurations when evaluating smooth muscle function. Given the physiologic relevance of axial extension and luminal pressurization, we submit that, when possible, axially isometric plus isobaric testing should be employed to evaluate vascular smooth muscle contractile function.

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