Efficient hydrolysis of raw starch by a maltohexaose-forming α-amylase from Corallococcus sp. EGB

LWT ◽  
2021 ◽  
Vol 152 ◽  
pp. 112361
Author(s):  
Lei Zhang ◽  
Lingli Zhong ◽  
Juying Wang ◽  
Yuqiang Zhao ◽  
Yiwei Zhang ◽  
...  
Keyword(s):  
Raw Starch ◽  
Hydrolysis Of

Raw starch degrading α-amylases: an unsolved riddle

Amylase ◽  
2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Nataša Božić ◽  
Nikola Lončar ◽  
Marinela Šokarda Slavić ◽  
Zoran Vujčić
Keyword(s):  
Glycoside Hydrolase Family ◽  
Food Ingredient ◽  
Raw Starch ◽  
Functional Studies ◽  
Structures And Properties ◽  
Structural Peculiarities ◽  
To Come ◽  
Starch Conversion ◽  
Maltose Syrup ◽  
Hydrolysis Of

AbstractStarch is an important food ingredient and a substrate for the production of many industrial products. Biological and industrial processes involve hydrolysis of raw starch, such as digestion by humans and animals, starch metabolism in plants, and industrial starch conversion for obtaining glucose, fructose and maltose syrup or bioethanol. Raw starch degrading α-amylases (RSDA) can directly degrade raw starch below the gelatinization temperature of starch. Knowledge of the structures and properties of starch and RSDA has increased significantly in recent years. Understanding the relationships between structural peculiarities and properties of RSDA is a prerequisite for efficient application in different aspects of human benefit from health to the industry. This review summarizes recent advances on RSDA research with emphasizes on representatives of glycoside hydrolase family GH13. Definite understanding of raw starch digesting ability is yet to come with accumulating structural and functional studies of RSDA.

DORMANCY STUDIES IN SEED OF AVENA FATUA: 3. A RELATIONSHIP BETWEEN MALTASE, AMYLASES, AND GIBBERELLIN

1962 ◽  
Vol 40 (12) ◽  
pp. 1659-1673 ◽  
Author(s):  
G. M. Simpson ◽  
J. M. Naylor
Keyword(s):  
Energy Source ◽  
Gibberellic Acid ◽  
Avena Fatua ◽  
Starch Granules ◽  
Raw Starch ◽  
Endosperm Starch ◽  
Hydrolysis Of ◽  
Exogenous Sugar ◽  
Excised Embryos

Initiation of germination in excised embryos requires an exogenous energy source. Normally this is obtained from the endosperm. In dormant seeds the hydrolysis of starch is blocked despite the fact that dormant and non-dormant seeds contain similar amounts of α- and β-amylases. Alone or in combination the amylases are unable to break down raw endosperm starch granules to simple sugars in vitro. Exogenous maltase in combination with α-amylase hydrolyzes raw starch to glucose. Exogenous maltase eliminates the requirement for exogenous sugar. Examination of the maltase content of imbibed dormant and non-dormant seeds showed a marked increase in non-dormant seeds during the first 40 hours. This does not occur in dormant seeds unless they are treated with gibberellic acid. The results lead to the conclusion that an important effect of gibberellic acid is to induce the synthesis of maltase or in some way activate the preformed enzyme.

scholarly journals A thin layer chromatographic comparison of raw and soluble starch hydrolysis patterns of some α-amylases from Bacillus sp. isolated in Serbia

2014 ◽  
Vol 79 (4) ◽  
pp. 411-420 ◽  
Author(s):  
Nikola Gligorijevic ◽  
Nikola Stevanovic ◽  
Nikola Loncar ◽  
Rada Baosic ◽  
Zoran Vujcic ◽  
...  
Keyword(s):  
Corn Starch ◽  
Soluble Starch ◽  
Starch Hydrolysis ◽  
Bacillus Sp ◽  
Raw Starch ◽  
Raw Starch Hydrolysis ◽  
Different Temperatures ◽  
Natural Isolates ◽  
Tlc Analysis ◽  
Hydrolysis Of

Several natural isolates of Bacillus strains namely 5B, 12B, 16B, 18 and 24B were grown on two different temperatures in submerged fermentation for the raw-starch-digesting a-amylases production. All strains except Bacillus sp. 18 produced more ?-amylase on 37?C. The hydrolysis of raw corn starch followed same pattern. Efficient hydrolysis was obtained with ?-amylases from Bacillus sp. 5B, 12B, 16B and 24B grown on 37?C and Bacillus sp. 18 grown on 50?C. Zymography after isoelectric focusing shown that ?-amylases were produced in multiple forms, from 2 to 6, depending on the strain when they were growing at 37 ?C, while growing at 50?C induced only 1 or 2 isoforms. TLC analysis of hydrolysis products of raw corn and soluble starch by ?-amylases revealed production of various mixtures of oligosaccharides. In most cases G3 was the most dominant product from soluble starch while G2, G3 and G5 were the main products of raw starch hydrolysis. This indicates that obtained a-amylases can be used for starch liquefying or short-chain-oligosaccharide forming, depending on what type of starch (raw or soluble) was used for the hydrolysis.

scholarly journals Repeated hydrolysis of raw starch using amylase immobilized on a reversibly soluble-insoluble carrier.

1989 ◽  
Vol 22 (1) ◽  
pp. 54-59 ◽  
Author(s):  
Kazuhiro Hoshino ◽  
Masayuki Taniguchi ◽  
Yoshiaki Netsu ◽  
Michihiro Fujii
Keyword(s):  
Raw Starch ◽  
Hydrolysis Of

scholarly journals Growth temperature of different local isolates of Bacillus sp. in the solid state affects production of raw starch digesting amylases

2014 ◽  
Vol 66 (2) ◽  
pp. 483-490
Author(s):  
Marinela Sokarda-Slavic ◽  
Natasa Bozic ◽  
Z. Vujcic
Keyword(s):  
Solid State ◽  
Amylase Activity ◽  
Agar Plate ◽  
Bacillus Sp ◽  
Wild Type ◽  
Raw Starch ◽  
Total Enzyme Activity ◽  
Hydrolysis Of ◽  
Type Strains ◽  
Total Enzyme

Natural amylase producers, wild type strains of Bacillus sp., were isolated from different regions of Serbia. Strains with the highest amylase activity based on the starch-agar plate test were grown on solid-state fermentation (SSF) on triticale. The influence of the substrate and different cultivation temperature (28 and 37?C) on the production of amylase was examined. The tested strains produced ?-amylases when grown on triticale grains both at 28 and at 37?C, but the activity of amylases and the number and intensity of the produced isoforms were different. Significant hydrolysis of raw cornstarch was obtained with the Bacillus sp. strains 2B, 5B, 18 and 24B. The produced ?-amylases hydrolyzed raw cornstarch at a temperature below the temperature of gelatinization, but the ability for hydrolysis was not directly related to the total enzyme activity, suggesting that only certain isoforms are involved in the hydrolysis.

Valorization of Residual Agroindustrial Cakes by Fungal Production of Multienzyme Complexes and Their Use in Cold Hydrolysis of Raw Starch

2011 ◽  
Vol 2 (3) ◽  
pp. 291-302 ◽  
Author(s):  
Aline Machado de Castro ◽  
Thiago Vetter de Andréa ◽  
Daniele Fernandes Carvalho ◽  
Mariana Martins Pereira Teixeira ◽  
Leda dos Reis Castilho ◽  
...  
Keyword(s):  
Raw Starch ◽  
Multienzyme Complexes ◽  
Hydrolysis Of

Semi-continuous Hydrolysis of Sweet Potato Raw Starch by Chalara paradoxa Glucoamylase

1992 ◽  
Vol 57 (6) ◽  
pp. 1348-1352 ◽  
Author(s):  
TAKAHIRO NODA ◽  
TOSHIO OHTANI ◽  
TAKEO SHIINA ◽  
YOSHIHIKO NAWA
Keyword(s):  
Sweet Potato ◽  
Raw Starch ◽  
Hydrolysis Of
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