Semi-continuous Hydrolysis of Sweet Potato Raw Starch by Chalara paradoxa Glucoamylase

1992 ◽  
Vol 57 (6) ◽  
pp. 1348-1352 ◽  
Author(s):  
TAKAHIRO NODA ◽  
TOSHIO OHTANI ◽  
TAKEO SHIINA ◽  
YOSHIHIKO NAWA
Keyword(s):  
Sweet Potato ◽  
Raw Starch ◽  
Hydrolysis Of

Raw starch degrading α-amylases: an unsolved riddle

Amylase ◽  
2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Nataša Božić ◽  
Nikola Lončar ◽  
Marinela Šokarda Slavić ◽  
Zoran Vujčić
Keyword(s):  
Glycoside Hydrolase Family ◽  
Food Ingredient ◽  
Raw Starch ◽  
Functional Studies ◽  
Structures And Properties ◽  
Structural Peculiarities ◽  
To Come ◽  
Starch Conversion ◽  
Maltose Syrup ◽  
Hydrolysis Of

AbstractStarch is an important food ingredient and a substrate for the production of many industrial products. Biological and industrial processes involve hydrolysis of raw starch, such as digestion by humans and animals, starch metabolism in plants, and industrial starch conversion for obtaining glucose, fructose and maltose syrup or bioethanol. Raw starch degrading α-amylases (RSDA) can directly degrade raw starch below the gelatinization temperature of starch. Knowledge of the structures and properties of starch and RSDA has increased significantly in recent years. Understanding the relationships between structural peculiarities and properties of RSDA is a prerequisite for efficient application in different aspects of human benefit from health to the industry. This review summarizes recent advances on RSDA research with emphasizes on representatives of glycoside hydrolase family GH13. Definite understanding of raw starch digesting ability is yet to come with accumulating structural and functional studies of RSDA.

DORMANCY STUDIES IN SEED OF AVENA FATUA: 3. A RELATIONSHIP BETWEEN MALTASE, AMYLASES, AND GIBBERELLIN

1962 ◽  
Vol 40 (12) ◽  
pp. 1659-1673 ◽  
Author(s):  
G. M. Simpson ◽  
J. M. Naylor
Keyword(s):  
Energy Source ◽  
Gibberellic Acid ◽  
Avena Fatua ◽  
Starch Granules ◽  
Raw Starch ◽  
Endosperm Starch ◽  
Hydrolysis Of ◽  
Exogenous Sugar ◽  
Excised Embryos

Initiation of germination in excised embryos requires an exogenous energy source. Normally this is obtained from the endosperm. In dormant seeds the hydrolysis of starch is blocked despite the fact that dormant and non-dormant seeds contain similar amounts of α- and β-amylases. Alone or in combination the amylases are unable to break down raw endosperm starch granules to simple sugars in vitro. Exogenous maltase in combination with α-amylase hydrolyzes raw starch to glucose. Exogenous maltase eliminates the requirement for exogenous sugar. Examination of the maltase content of imbibed dormant and non-dormant seeds showed a marked increase in non-dormant seeds during the first 40 hours. This does not occur in dormant seeds unless they are treated with gibberellic acid. The results lead to the conclusion that an important effect of gibberellic acid is to induce the synthesis of maltase or in some way activate the preformed enzyme.

Hydrolysis of Aryl β-Maltotriosides by Sweet Potato β-Amylase and Soybean β-Amylase

1978 ◽  
Vol 83 (2) ◽  
pp. 473-478 ◽  
Author(s):  
Nobuyuki SUETSUGU ◽  
Ken'ichi TAKEO ◽  
Yutaka SANAI ◽  
Takashi KUGE
Keyword(s):  
Sweet Potato ◽  
Hydrolysis Of

scholarly journals A thin layer chromatographic comparison of raw and soluble starch hydrolysis patterns of some α-amylases from Bacillus sp. isolated in Serbia

2014 ◽  
Vol 79 (4) ◽  
pp. 411-420 ◽  
Author(s):  
Nikola Gligorijevic ◽  
Nikola Stevanovic ◽  
Nikola Loncar ◽  
Rada Baosic ◽  
Zoran Vujcic ◽  
...  
Keyword(s):  
Corn Starch ◽  
Soluble Starch ◽  
Starch Hydrolysis ◽  
Bacillus Sp ◽  
Raw Starch ◽  
Raw Starch Hydrolysis ◽  
Different Temperatures ◽  
Natural Isolates ◽  
Tlc Analysis ◽  
Hydrolysis Of

Several natural isolates of Bacillus strains namely 5B, 12B, 16B, 18 and 24B were grown on two different temperatures in submerged fermentation for the raw-starch-digesting a-amylases production. All strains except Bacillus sp. 18 produced more ?-amylase on 37?C. The hydrolysis of raw corn starch followed same pattern. Efficient hydrolysis was obtained with ?-amylases from Bacillus sp. 5B, 12B, 16B and 24B grown on 37?C and Bacillus sp. 18 grown on 50?C. Zymography after isoelectric focusing shown that ?-amylases were produced in multiple forms, from 2 to 6, depending on the strain when they were growing at 37 ?C, while growing at 50?C induced only 1 or 2 isoforms. TLC analysis of hydrolysis products of raw corn and soluble starch by ?-amylases revealed production of various mixtures of oligosaccharides. In most cases G3 was the most dominant product from soluble starch while G2, G3 and G5 were the main products of raw starch hydrolysis. This indicates that obtained a-amylases can be used for starch liquefying or short-chain-oligosaccharide forming, depending on what type of starch (raw or soluble) was used for the hydrolysis.

scholarly journals Repeated hydrolysis of raw starch using amylase immobilized on a reversibly soluble-insoluble carrier.

1989 ◽  
Vol 22 (1) ◽  
pp. 54-59 ◽  
Author(s):  
Kazuhiro Hoshino ◽  
Masayuki Taniguchi ◽  
Yoshiaki Netsu ◽  
Michihiro Fujii
Keyword(s):  
Raw Starch ◽  
Hydrolysis Of

scholarly journals Potential Utilization of Low Quality Sweet Potato for Bioethanol Production by Saccharomyces cerevisiae TISTR5339

2020 ◽  
Vol 17 (9) ◽  
pp. 933-946
Author(s):  
Supasit CHOOKLIN ◽  
Parichat NINUP-PATHAM ◽  
Saovanee CHOOJIT
Keyword(s):  
Ethanol Production ◽  
Acid Hydrolysis ◽  
Sweet Potato ◽  
Ammonium Sulphate ◽  
Ethanol Yield ◽  
Scale Up ◽  
Inoculum Size ◽  
Sulphate Content ◽  
Box Behnken Design ◽  
Hydrolysis Of

This study was aimed to investigate the optimal condition of ethanol production that has 2 major stages: acid hydrolysis and fermentation processes. These processes came from low quality sweet potato (LQSP) which was destroyed by the sweet potato weevil. The main compositions of LQSP were starch and fiber which consist of 55.25 and 10.29 %, respectively. In this case, the starch can be hydrolyzed to reduce the sugar, followed by the fermentation of the reduced sugar to ethanol. For this experiment, the effecting factors on acid hydrolysis of LQSP and the ethanol fermentation condition were optimized by S. cerevisiae using Response Surface Methodology (RSM) with Box-Behnken design in order to maximize ethanol yield. It was found that the maximum reducing sugar concentration of 390.99 ± 5.35 g/L was obtained from the hydrolysis condition with 1 % (v/v) of sulfuric acid and 25 % (w/v) of LQSP. Accordingly, the effects of ammonium sulphate content (0.05 - 0.15 %), pH (4.5 - 5.5) and inoculum content (5 - 10 %) on ethanol production was determined by RSM using Box-Behnken experiment design with a total 17 sets of all trials. The results were found that the maximum experimental ethanol productivity of 5.98 g/L was obtained from the condition at 0.05 % of ammonium sulphate, pH 5.5 and 5.0 % of inoculum size to 90 mL LQSP based medium and incubated at 30 °C for 48 h. In addition, the scale-up of ethanol production was studied in 9 L fermenter which provided the maximum ethanol yield of 5.04 g/L. Therefore, it can be concluded that LQSP had a potential as a substrate for ethanol production.

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