Production of aminopeptidase from soybean meal with nutrients supplementation by Bacillus licheniformis SWJS33: Feasibility and metabolic process

Periplasm of bacteria is the space outside the permeability barrier of plasma membrane but enclosed by the cell wall. The contents of this special milieu exterior could be regulated by the plasma membrane from the internal, and by the cell wall from the external environment of the cell. Unlike the gram-negative organism, the presence of this space in gram-positive bacteria is still controversial because it cannot be clearly demonstrated. We have shown the importance of some periplasmic bodies in the secretion of penicillinase from Bacillus licheniformis.In negatively stained specimens prepared by a modified technique (Figs. 1 and 2), periplasmic space (PS) contained two kinds of structures: (i) fibrils (F, 100 Å) running perpendicular to the cell wall from the protoplast and (ii) an array of vesicles of various sizes (V), which seem to have evaginated from the protoplast.

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Interrelationship of the cellular distribution and secretion of regular structure (RS) protein in Bacillus licheniformis nm 105

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100123969 ◽

1992 ◽

Vol 50 (1) ◽

pp. 712-713

Author(s):

Xiaorong Zhu ◽

Richard McVeigh ◽

Bijan K. Ghosh

Keyword(s):

Alkaline Phosphatase ◽

Bacillus Licheniformis ◽

Self Assembly ◽

Gel Electrophoresis ◽

Culture Supernatant ◽

Regular Structure ◽

The Self ◽

Cellular Distribution ◽

Structural Protein ◽

Laboratory Cultures

A mutant of Bacillus licheniformis 749/C, NM 105 exhibits some notable properties, e.g., arrest of alkaline phosphatase secretion and overexpression and hypersecretion of RS protein. Although RS is known to be widely distributed in many microbes, it is rarely found, with a few exceptions, in laboratory cultures of microorganisms. RS protein is a structural protein and has the unusual properties to form aggregate. This characteristic may have been responsible for the self assembly of RS into regular tetragonal structures. Another uncommon characteristic of RS is that enhanced synthesis and secretion which occurs when the cells cease to grow. Assembled RS protein with a tetragonal structure is not seen inside cells at any stage of cell growth including cells in the stationary phase of growth. Gel electrophoresis of the culture supernatant shows a very large amount of RS protein in the stationary culture of the B. licheniformis. It seems, Therefore, that the RS protein is cotranslationally secreted and self assembled on the envelope surface.

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In vivo measurement of flatulence and nutrient digestibility in dogs fed poultry by-product meal, conventional soybean meal, and low-oligosaccharide low-phytate soybean meal

Journal of the American Veterinary Medical Association ◽

10.2460/javma.228.2.235 ◽

2006 ◽

Vol 228 (2) ◽

pp. 235-235

Author(s):

Ryan M. Yamka ◽

David L. Harmon ◽

William D. Schoenherr ◽

Christina Khoo ◽

Kathy L. Gross ◽

...

Keyword(s):

Soybean Meal ◽

Nutrient Digestibility ◽

In Vivo Measurement

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Japon Bıldırcını Rasyonlarında Soya Küspesi Yerine Pamuk Tohumu Küspesi İkamesinin Yumurta Verim ve Kalite Özelliklerine Etkisi

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v1i1.44-50.19 ◽

2013 ◽

Vol 1 (1) ◽

pp. 44 ◽

Cited By ~ 2

Author(s):

Arda Yıldırım ◽

Ergin Öztürk

Keyword(s):

Protein Content ◽

Cottonseed Meal ◽

Soybean Meal ◽

Shell Thickness ◽

Egg Production ◽

Egg Quality ◽

Yield Traits

This study was conducted to determine the effect of cottonseed meal (CSM) incorporated into laying rations in place of soybean meal (SBM) at different ratios on yield traits. The birds began to lay at 6th week, 180 female and 45 male quails were used in laying period experiment. Birds were fed with rations containing 20% CP and 3000 Kcal/kg ME up to 20-week age (Laying period). CSM as a substitute, five different rations of the protein content (0, 30, 58, 86 and 100%) for SBM to basal diets based on corn-soybean meal were used. The results showed that there were no differences in terms of egg yield traits, cumulative feed consumptions and viabilities during the laying period. The highest dry shell rate and shell thickness were obtained from 5th group and 1st group, respectively. As a result, adding CSM instead of SBM in laying period were no significantly differences in terms of egg production and egg quality in laying period.

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