scholarly journals The β-galactosidase LacLM plays the major role in lactose utilization of Lactiplantibacillus plantarum

LWT ◽  
2021 ◽  
pp. 112481
Author(s):  
Zhenshang Xu ◽  
Can Li ◽  
Yanxin Ye ◽  
Ting Wang ◽  
Susu Zhang ◽  
...  
Keyword(s):  

Genetics ◽  
1997 ◽  
Vol 146 (3) ◽  
pp. 751-767 ◽  
Author(s):  
Timothy Galitski ◽  
John R Roth

Homologous recombination pathways probably evolved primarily to accomplish chromosomal repair and the formation and resolution of duplications by sister-chromosome exchanges. Various DNA lesions initiate these events. Classical recombination assays, involving bacterial sex, focus attention on double-strand ends of DNA. Sexual exchanges, initiated at these ends, depend on the RecBCD pathway. In the absence of RecBCD function, mutation of the sbcB and sbcC genes activates the apparently cryptic RecF pathway. To provide a more general view of recombination, we describe an assay in which endogenous DNA damage initiates recombination between chromosomal direct repeats. The repeats flank markers conferring lactose utilization (Lac+) and ampicillin resistance (ApR); recombination generates Lac-ApS segregants. In this assay, the RecF pathway is not cryptic; it plays a major role without sbcBC mutations. Others have proposed that single-strand gaps are the natural substrate for RecF-dependent recombination. Supporting this view, recombination stimulated by a double-strand break (DSB) in a chromosomal repeat depended on RecB function, not RecF function. Without RecBCD function, sbcBC mutations modified the RecF pathway and allowed it to catalyze DSB-stimulated recombination. Sexual recombination assays overestimate the importance of RecBCD and DSBs, and underestimate the importance of the RecF pathway.



1978 ◽  
Vol 24 (4) ◽  
pp. 372-380 ◽  
Author(s):  
P. L. Rogers ◽  
L. Bramall ◽  
I. J. McDonald

The growth of Streptococcus cremoris on a semidefined medium was studied at initial lactose concentrations of 0.2–5.0% in batch culture, and in lactose-limited chemostat cultures at 0.5% lactose. Kinetic analysis of the batch data, using statistical techniques, indicated the importance of lactose limitation and lactic acid inhibition of the growth of S. cremoris. A model for the biomass production, lactose utilization, and lactic acid production in batch culture was proposed. In continuous culture, it was found that steady state populations were maintained at higher dilution rates (D = 0.6–0.7 h−1) than the maximum predicted by batch culture (0.56 h−1). No evidence for a selection of fast-growing mutants was obtained. Copious growth adhering to the walls of the fermentor (i.e. wall growth) occurred very rapidly at higher dilution rates and this undoubtedly affected steady-state growth and wash-out and, as a consequence, the apparent maximum dilution rate.



2017 ◽  
Vol 2 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Aladár Vidra ◽  
András József Tóth ◽  
Áron Németh

Abstract Whey is the complex waste of the dairy industry. Despite the fact, that it has numerous applications (like different form of food supplements), its major amount is still handled as waste. The carbohydrate, protein and lactic acid content, as well as the COD and BOD, are sufficiently high warranting disposal as waste resulting in high costs; however, their levels are insufficient for the cost-effective isolation and purification. Most of the numerous reports on whey utilisation focus on lactose utilization, while lactic acid removal is complex, but necessary, particularly in case of sour whey decontamination. According to our best knowledge among the microbial fermentation, the only lactic acid (as carbon source) utilization process is propionic acid fermentation. Propionic acid is an attractive product with a wide application range. In this study, two propionic acid producing microorganisms were investigated in terms of industrial applicability. The propionic acid producing bacteria are generally characterized by anaerobic metabolism (except the pathogenic P. acne); but, for application in a biorefinery, facultative anaerobe behavior is the most appropriate and cost-effective. In this study, the aero-tolerances of Propionibacterium freudenreichii subsp. shermanii and Propionibacterium acidipropionici were examined; their propionic acid-producing properties (yield, concentration, substrate preference, productivity) were compared.



2020 ◽  
Vol 103 (1) ◽  
pp. 166-171
Author(s):  
Peng Yu ◽  
Nan Li ◽  
Mingxue Geng ◽  
Zhenmin Liu ◽  
Xiaoming Liu ◽  
...  


2002 ◽  
Vol 44 (2) ◽  
pp. 102-105 ◽  
Author(s):  
Gwo-Chyuan Shaw ◽  
Chih-Yung Chiou ◽  
Yi-Hua Chou ◽  
Jen-Ming Li


2006 ◽  
Vol 97 (18) ◽  
pp. 2252-2259 ◽  
Author(s):  
Nahit Aktaş ◽  
İ. Hakkı Boyacı ◽  
Mehmet Mutlu ◽  
Abdurrahman Tanyolaç




1964 ◽  
Vol 37 (1) ◽  
pp. 81-98 ◽  
Author(s):  
R. DAVIES
Keyword(s):  


1996 ◽  
Vol 203 (1) ◽  
pp. 77-81 ◽  
Author(s):  
Jes�s Rodr�guez ◽  
Teresa Requena ◽  
Isabel Mart�nez-Castro ◽  
Manuela Ju�rez
Keyword(s):  


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