scholarly journals Complex whey utilization: the propionic acid alternative

2017 ◽  
Vol 2 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Aladár Vidra ◽  
András József Tóth ◽  
Áron Németh
Keyword(s):  
Lactic Acid ◽  
Propionic Acid ◽  
Cost Effective ◽  
Acid Fermentation ◽  
Isolation And Purification ◽  
Application Range ◽  
Lactic Acid Content ◽  
Lactose Utilization ◽  
Acid Producing Bacteria ◽  
The Cost

Abstract Whey is the complex waste of the dairy industry. Despite the fact, that it has numerous applications (like different form of food supplements), its major amount is still handled as waste. The carbohydrate, protein and lactic acid content, as well as the COD and BOD, are sufficiently high warranting disposal as waste resulting in high costs; however, their levels are insufficient for the cost-effective isolation and purification. Most of the numerous reports on whey utilisation focus on lactose utilization, while lactic acid removal is complex, but necessary, particularly in case of sour whey decontamination. According to our best knowledge among the microbial fermentation, the only lactic acid (as carbon source) utilization process is propionic acid fermentation. Propionic acid is an attractive product with a wide application range. In this study, two propionic acid producing microorganisms were investigated in terms of industrial applicability. The propionic acid producing bacteria are generally characterized by anaerobic metabolism (except the pathogenic P. acne); but, for application in a biorefinery, facultative anaerobe behavior is the most appropriate and cost-effective. In this study, the aero-tolerances of Propionibacterium freudenreichii subsp. shermanii and Propionibacterium acidipropionici were examined; their propionic acid-producing properties (yield, concentration, substrate preference, productivity) were compared.

The effect of wilting on butyric acid fermentation in silage.

1958 ◽  
Vol 6 (3) ◽  
pp. 204-210 ◽  
Author(s):  
G.W. Wieringa
Keyword(s):  
Lactic Acid ◽  
Osmotic Pressure ◽  
Butyric Acid ◽  
Ion Concentration ◽  
Acid Fermentation ◽  
Silage Fermentation ◽  
Lactic Acid Content ◽  
Vitro Development ◽  
Butyric Acid Fermentation

A comparatively slight increase in environmental osmotic pressure inhibited the in vitro development of butyric-acid bacteria [Clostridium spp.], especially with increasing H-ion concentration. Sub-lethal concentrations of salt (NaCl, KCl, Na2SO4) and/or H-ions retarded the start of clostridial development and reduced the quantity of butyric acid produced. In wilted grass silage it was shown that osmotic pressure plays a considerable part in repressing butyric-acid fermentation in the initial stages of silage fermentation. Low temperature (< 20-25 degrees C.), low pH (< 4.2), high lactic-acid content and high osmotic pressure were more harmful to clostridia than to lactic-acid bacteria.-R.B. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Bacterial and Fungal Microbiota Associated with the Ensiling of Wet Soybean Curd Residue under Prompt and Delayed Sealing Conditions

2020 ◽  
Vol 8 (9) ◽  
pp. 1334
Author(s):  
Ajmal Wali ◽  
Naoki Nishino
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Fermentation ◽  
Acid Fermentation ◽  
Fermentation Products ◽  
Soybean Curd ◽  
Bacillus Spp ◽  
Acinetobacter Spp ◽  
Fungal Microbiota ◽  
Acid Producing Bacteria ◽  
Soybean Curd Residue

Wet soybean curd residue (SCR) obtained from two tofu factories (F1 and F2) was anaerobically stored with or without added beet pulp (BP). Sealing was performed on the day of tofu production (prompt sealing (PS)) or 2 days after SCR was piled and unprocessed (delayed sealing (DS)). Predominant lactic acid fermentation was observed regardless of the sealing time and BP addition. Acinetobacter spp. were the most abundant (>67%) bacteria in pre-ensiled SCR, regardless of the factory and sealing time. In PS silage, the abundances of typical lactic acid-producing bacteria, such as Lactobacillus, Pediococcus, and Streptococcus spp. reached >50%. In DS silage, Acinetobacter spp. were the most abundant in F1 products, whereas Bacillus spp. were the most abundant in long-stored F2 products. The fungal microbiota were highly diverse. Although Candida, Aspergillus, Cladosporium, Hannaella, and Wallemia spp. were found to be the most abundant fungal microbiota, no specific genera were associated with factory, sealing time, or fermentation products. These results indicated that owing to preceding processing, including heating, distinctive microbiota may have participated in the ensiling of wet by-products. Lactic acid fermentation was observed even in DS silage, and an association of Bacillus spp. was suggested.

scholarly journals Production of Lactic Acid from Seaweed Hydrolysates via Lactic Acid Bacteria Fermentation

Fermentation ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 37 ◽  
Author(s):  
Hong-Ting Victor Lin ◽  
Mei-Ying Huang ◽  
Te-Yu Kao ◽  
Wen-Jung Lu ◽  
Hsuan-Ju Lin ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Sugar Content ◽  
Carbon Sources ◽  
Lactic Acid Fermentation ◽  
Microbial Fermentation ◽  
Acid Fermentation ◽  
High Production Cost ◽  
Fermentation Parameters ◽  
The Cost

Biodegradable polylactic acid material is manufactured from lactic acid, mainly produced by microbial fermentation. The high production cost of lactic acid still remains the major limitation for its application, indicating that the cost of carbon sources for the production of lactic acid has to be minimized. In addition, a lack of source availability of food crop and lignocellulosic biomass has encouraged researchers and industries to explore new feedstocks for microbial lactic acid fermentation. Seaweeds have attracted considerable attention as a carbon source for microbial fermentation owing to their non-terrestrial origin, fast growth, and photoautotrophic nature. The proximate compositions study of red, brown, and green seaweeds indicated that Gracilaria sp. has the highest carbohydrate content. The conditions were optimized for the saccharification of the seaweeds, and the results indicated that Gracilaria sp. yielded the highest reducing sugar content. Optimal lactic acid fermentation parameters, such as cell inoculum, agitation, and temperature, were determined to be 6% (v/v), 0 rpm, and 30 °C, respectively. Gracilaria sp. hydrolysates fermented by lactic acid bacteria at optimal conditions yielded a final lactic acid concentration of 19.32 g/L.

scholarly journals Considerations on the Dynamics of the pH Values and Lactic Acid Content during Lactic Acid Fermentation of Carrot Juices with Different Pure Cultures

1970 ◽  
Vol 66 (2) ◽  
Author(s):  
Gabriel GORGHIU ◽  
Lavinia BURULEANU ◽  
Carmen Leane NICOLESCU ◽  
Daniela AVRAM
Keyword(s):  
Lactic Acid ◽  
Acid Content ◽  
Lactic Acid Fermentation ◽  
Regression Equations ◽  
Acid Fermentation ◽  
Software Application ◽  
Related Data ◽  
Ph Values ◽  
Lactic Acid Content ◽  
Pure Cultures

Suitability of the carrots for obtaining lactic acid fermented juices using different lactic acid bacteria and Bifidobacterium sp. that are not constitutive from the epiphytic microbiota of vegetables was tested. One the one hand, the aim of this work was to obtain related data to the dynamics of the titrable acidity and the pH during the course of the controlled fermentation, due of the importance of these parameters on the shelf life of the final products. Optimum type of culture and optimum amount of inoculum was determined. On the other hand, the work proposes also model predictions for expressing the pH values and the lactic acid content dynamics, during lactic acid fermentation of carrot juices with different pure cultures. Using the CurveExpert 1.3 software application, specific regression equations were determined.

Biogenic Amine Formation and “Zapatera” Spoilage of Fermented Green Olives: Effect of Storage Temperature and Debittering Process

2004 ◽  
Vol 67 (1) ◽  
pp. 117-123 ◽  
Author(s):  
P. GARCÍA GARCÍA ◽  
C. ROMERO BARRANCO ◽  
M. C. DURÁN QUINTANA ◽  
A. GARRIDO FERNÁNDEZ
Keyword(s):  
Lactic Acid ◽  
Propionic Acid ◽  
Biogenic Amine ◽  
Acid Content ◽  
Storage Temperature ◽  
Table Olives ◽  
Chemical Changes ◽  
Lactic Acid Content ◽  
Effects Of Temperature ◽  
Practical Stabilization

The effects of temperature and the debittering process on amine formation and other chemical changes related to “zapatera” spoilage of fermented green table olives during storage, without any chemical correction, were studied. Unwashed olive brines were more concentrated in all analyzed compounds, except NaCl. No changes in formic, acetic, and succinic acids or in ethanol, hydroxytyrosol, or tyrosol were observed in the olive brines during storage. The concentration of putrescine in the brine at the beginning of storage and end of fermentation was about 38 mg/liter, and it did not change during storage. This amine only seems to be produced during the active fermentation phase. The effects of temperature and the type of debittering process and time and its interactions (except the time × temperature × debittering process on pH) had signi cant effects on the production of cadaverine and tyramine, as well as on changes of pH and lactic and propionic acids. Storage at 15°C produced a complete stabilization of the fermented olives. However, storage of washed olives at 20 and 28°C produced a gradual decrease of lactic acid content, an increase in pH, production of propionic acid, and formation of cadaverine and tyramine, the effect becoming greater as the temperature rose. It appears that formation of cadaverine and tyramine only occurs during storage and might be related to zapatera spoilage. Changes were always significantly lower in unwashed olives, which leads to a practical stabilization of the product.

scholarly journals Mildly acidic pH selects for chain elongation over propionic acid production in lactic acid fermentation

2020 ◽  
Author(s):  
Pieter Candry
Keyword(s):  
Lactic Acid ◽  
Propionic Acid ◽  
Acid Production ◽  
Lactic Acid Fermentation ◽  
Chain Elongation ◽  
Acidic Ph ◽  
Acid Fermentation ◽  
Propionic Acid Production ◽  
The Right

Contribution to the International Chain Elongation Conference 2020 | ICEC 2020. An abstract can be found in the right column.

Reactive Extraction of Propionic Acid Using Tri-n-octylamine

2012 ◽  
Vol 550-553 ◽  
pp. 634-638 ◽  
Author(s):  
Seong Ho Jung ◽  
Ki Sub Kim ◽  
Yeon Ki Hong ◽  
Byung Heung Park
Keyword(s):  
Propionic Acid ◽  
Equilibrium Distribution ◽  
Fermentation Broth ◽  
Cost Effective ◽  
Recovery Process ◽  
Separation Process ◽  
Reactive Extraction ◽  
Chemical Products ◽  
The Cost ◽  
Long Chain

Propionic acid has been received increasing attention due to its various usages as an antifungal agent in food and a chemical in the production of several chemical products. In order to develop an alternative production process of propionic acid such as fermentation of glycerol, the cost effective recovery process of propionic acid from its fermentation broth is needed. Differently from conventional physical extraction, long chain amine (TOA)-based extraction is the separation process using reactions between amine and materials extracted. The equilibrium distribution of propionic acid increased with amine concentration and decreased with increase of n-heptane composition in mixed diluents. From the loading values with TOA concentrations, it was found that the stoichiometries of acid-amine-1-octanol complex were (1,1) and (1,1,1). From this study, amine-based extraction can be promising separation process for the recovery of propionic acid.

scholarly journals Isolation of nucleic acids using silicon dioxide powder as a tool for environmental monitoring

2019 ◽  
Vol 191 (12) ◽  
Author(s):  
Jacek Urbaniak ◽  
Daniel Janowski ◽  
Brayan Jacewski
Keyword(s):  
Nucleic Acids ◽  
Silicon Dioxide ◽  
Environmental Monitoring ◽  
Cost Effective ◽  
Ease Of Use ◽  
Isolation And Purification ◽  
Long Run ◽  
Dioxide Powder ◽  
Commercial Kits ◽  
The Cost

Abstract Isolation and purification of nucleic acids are basic laboratory procedures used in molecular analysis supporting determination of organisms in environmental monitoring. However, many different methods of isolation are commonly used, often being designed for a particular type of DNA extraction. While researchers commonly decide on commercial isolation kits for their ease of use and efficiency, they require large amounts of studied tissue, and the cost of purchasing such kits over a long run can be high. To provide an alternative to using commercial kits, we have developed a simple, rapid, cost-effective, and reliable protocol for DNA isolation from cultured fungi on slants and from dried fungal samples using silica particles (silicon dioxide powder) in chaotropic conditions. With the presented method, it is possible to isolate good-quality DNA from fungi in less than 1.5 h, using easily accessible chemicals. Compared with other methods employing CTAB or commercial kits, it allows fast, easy, and cheap DNA purification from two main sources of fungi routinely used for research. In addition to the method protocol, we also provide advice for further optimization of the isolation process to account for specific conditions, making the procedure more useful.

СРАВНИТЕЛЬНАЯ ЭФФЕКТИВНОСТЬ ПРИМЕНЕНИЯ РАЗЛИЧНЫХ БИОЛОГИЧЕСКИХ ПРЕПАРАТОВ ПРИ КОНСЕРВИРОВАНИИ ЛЮЦЕРНЫ

2019 ◽  
pp. 59-63
Author(s):  
I.T. BIKCHANTAEV ◽  
SH.K. SHAKIROV ◽  
Z.F. FATTAKHOVA
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Propionic Acid ◽  
Acid Production ◽  
Exchange Energy ◽  
Acid Fermentation ◽  
Green Mass ◽  
Propionic Acid Bacteria ◽  
Microbial Associations

Проведена сравнительная оценка эффективности влияния различных биологических препаратов при консервировании провяленной зеленой массы люцерны, отечественного так и зарубежного производства в лабораторных условиях. По результатам лабораторных исследований было установлено положительное влияние биологических препаратов ФербакСил и Бонсилаж Форте, которые стимулировали в фитомассе молочнокислое брожение, что в свою очередь положительно отразилось на сохранности обменной энергии (8,9 МДж) и сырого протеина (21,1 ), показатели которых были выше контроля на 3,45 и 0,9 . Максимальное кислотообразование в консервируемой массе выявлено при применении импортного препарата Бонсилаж Форте, где показатель суммы органических кислот был наивысшим и составил 3,66 абс. , или выше контрольного показателя на 0,33 абс. , соответственно. При изучении физиологических групп микробиоты готовых сенажей, в консервировании которых использовали биологические препараты, выявлено повышенное содержание молочнокислых и пропионовокислых бактерий. Максимальное содержание первых было установлено при применении препарата ФербакСил 58,8106 КОЕ/г, последних при применении Бонсилаж Форте 7,2106 КОЕ/г, концентрация которых была выше контроля на 3,3 и 2,4 раза. Расчет экономической эффективности показал, что использование препарата Бонсилаж Форте максимально увеличивает себестоимость готового сенажа по сравнению с контролем на 13,0 (1185 руб./т). Применение отечественного препарата ФербакСил способствовал минимальному увеличению себестоимости готового корма 1063 руб/т, стоимость которого была ниже контроля на 1,2 .The efficacy of two biological preparations was evaluated for haylage preparation from dried alfalpha green mass under laboratory conditions. It was shown that biopreparations FerbacSil and Bonehaylage Forte, stimulating lactic acid fermentation in green mass, improved quality of the haylage by preserving exchange energy (8,9 MJ), raw protein (21,1 ), which were higher than those in control by 3,45 and 0,9 , respectively. Maximal acid production in haylage mass was detected after application of Bonehaylage Forte reaching 3,66 absolute , so 0,33 abs. higher than in the control. Microbial associations developed in green mass were represented by lactic acid bacteria and propionic acid bacteria. Application of FerbacSil resulted in increase of by lactic acid bacteria population which reached 58,8106 CFU per gram of green mass. Propionic acid bacteria were proliferating to 7,2106 CFU per gram when Bonehaylage Forte was used as a silaging starter. These value were 3,3 and 2,4 higher, respectively, than in control variant. Calculation of profitability showed that Bonehaylage Forte application increase selfcost by 13 when compared with control and is as high as 1185 roubles per ton of haylage. Using Russian domestic biopreparation FerbacSil resulted in moderate 1,2 of increase of selfcost giving final 1063 roubles per ton, which is 1,2 lower than in control.

Sign in / Sign up

Export Citation Format

Share Document