The role and biology of senescent cells in ageing-related tissue damage and repair

Author(s):  
Markus Schosserer
Keyword(s):  
Author(s):  
Hilton H. Mollenhauer

Various means have been devised to preserve biological specimens for electron microscopy, the most common being chemical fixation followed by dehydration and resin impregnation. It is intuitive, and has been amply demonstrated, that these manipulations lead to aberrations of many tissue elements. This report deals with three parts of this problem: specimen dehydration, epoxy embedding resins, and electron beam-specimen interactions. However, because of limited space, only a few points can be summarized.Dehydration: Tissue damage, or at least some molecular transitions within the tissue, must occur during passage of a cell or tissue to a nonaqueous state. Most obvious, perhaps, is a loss of lipid, both that which is in the form of storage vesicles and that associated with tissue elements, particularly membranes. Loss of water during dehydration may also lead to tissue shrinkage of 5-70% (volume change) depending on the tissue and dehydrating agent.


Author(s):  
R. W. Cole ◽  
J. C. Kim

In recent years, non-human primates have become indispensable as experimental animals in many fields of biomedical research. Pharmaceutical and related industries alone use about 2000,000 primates a year. Respiratory mite infestations in lungs of old world monkeys are of particular concern because the resulting tissue damage can directly effect experimental results, especially in those studies involving the cardiopulmonary system. There has been increasing documentation of primate parasitology in the past twenty years.


2010 ◽  
Vol 48 (01) ◽  
Author(s):  
R Dayoub ◽  
C Dorn ◽  
C Hackl ◽  
O Stölzing ◽  
WE Thasler ◽  
...  

1987 ◽  
Vol 49 (1) ◽  
pp. 24-27 ◽  
Author(s):  
Keiko TANAKA ◽  
Yuji SHUDO ◽  
Akira TANAKA

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