scholarly journals UP Finder: A COBRA toolbox extension for identifying gene overexpression strategies for targeted overproduction

2017 ◽  
Vol 5 ◽  
pp. 54-59 ◽  
Author(s):  
Xi Wang ◽  
Liang Yu ◽  
Shulin Chen
LWT ◽  
2021 ◽  
pp. 111812
Author(s):  
Yu Lu ◽  
Xiangjin Cheng ◽  
Huanhuan Deng ◽  
Shouwen Chen ◽  
Zhixia Ji

2021 ◽  
Author(s):  
Madhab Kumar Sen ◽  
Katerina Hamouzová ◽  
Jakub Mikulka ◽  
Rohit Bharati ◽  
Pavlina Košnarová ◽  
...  

LWT ◽  
2021 ◽  
pp. 111699
Author(s):  
Hanieh Yarabbi ◽  
Seyed Ali Mortazavi ◽  
Masoud Yavarmanesh ◽  
Ali Javadmanesh

Mycoses ◽  
2017 ◽  
Vol 60 (7) ◽  
pp. 462-468 ◽  
Author(s):  
Marcos Fábio Gadelha Rocha ◽  
Silviane Praciano Bandeira ◽  
Lucas Pereira de Alencar ◽  
Luciana Magalhães Melo ◽  
Jamille Alencar Sales ◽  
...  

1994 ◽  
Vol 14 (11) ◽  
pp. 7581-7591
Author(s):  
K Kataoka ◽  
K T Fujiwara ◽  
M Noda ◽  
M Nishizawa

We have identified a new member of the maf oncogene family and named it mafB. This gene is expressed in a wide variety of tissues and encodes a protein of 311 amino acids containing a typical bZip motif in its carboxy-terminal region. In the bZip domain, MafB shares extensive homology not only with v-Maf but also with other Maf-related proteins. As expected from its structure, MafB forms a homodimer through its leucine repeat structure and specifically binds Maf-recognition elements (MAREs). In addition, MafB forms heterodimers with v-Maf and Fos through its zipper structure. However, unlike v-Maf, MafB fails to associate with Jun. Transient cotransfection assays revealed that both v-Maf and MafB act as transactivators for a promoter linked to MAREs, although MafB is less potent than v-Maf. As is the case for the c-maf gene, overexpression of the mafB gene induces transformation of chicken embryo fibroblasts in vitro. Through formation of numerous bZip dimers, the Maf family proteins along with the AP-1 components should provide great diversity in transcriptional regulation for a wide variety of genes.


1995 ◽  
Vol 62 (1) ◽  
pp. 84-89 ◽  
Author(s):  
Monica Binaschi ◽  
Rosanna Supino ◽  
Romolo A. Gambetta ◽  
Giuseppe Giaccone ◽  
Ennio Prosperi ◽  
...  

2022 ◽  
Vol 12 ◽  
Author(s):  
Youhui Gong ◽  
Ting Li ◽  
Qi Li ◽  
Shikai Liu ◽  
Nannan Liu

Mosquitoes’ increasing resistance to insecticides is becoming a major threat for control efforts worldwide. Multiple P450 genes that are up-regulated in permethrin resistant strains of Culex quinquefasciatus have been linked to the development of resistance. In the current study, we characterized the function of six P450 genes, CYP6P14, CYP6BZ2, CYP9J33, CYP9J34, CYP9J40, and CYP9J45, that are overexpressed in the permethrin resistant Culex mosquitoes and showed their capability in metabolism of permethrin. These six P450 genes can convert 3-phenoxybenzoic alcohol (PBCHO) to a less toxic product, 3-phenoxybenzoic acid (PBCOOH), indicating that these P450s play an important role in permethrin degradation pathways. Although we know multiple P450 genes are over-expressed in permethrin resistant Culex mosquitoes, it remains to be seen whether cytochrome P450-reductase (CPR) gene that are co-overexpressed with P450 genes in permethrin resistant mosquitoes do indeed serve as a resistance mechanism. An in-depth investigation of the expression of CPR gene in resistant mosquitoes was conducted in permethrin resistant mosquitoes. The finding of CPR gene overexpression in permethrin resistant mosquitoes suggested the importance of co-overexpression of multiple P450 genes with their obligatory electron donor CPR in the complex detoxification system, boosting the metabolism of permethrin and hence the development of permethrin resistance in Cx. quinquefasciatus.


Author(s):  
Salma Sarai González-Meléndez

<p>My name is Salma Meléndez and I am currently a graduate in Agrogenomic Sciences. In March 2020, when COVID-19 was detected in Mexico, I was in my eighth semester of my undergraduate degree. At that time, he had an experiment of overexpression of a gene in bean roots, in order to explore its function during symbiosis with rhizobial bacteria. Unfortunately, the laboratory and the entire campus canceled their face-to-face activities in order to reduce the risk of contagion. An alternative was to take the experimental plants to my house to give the proper care, however, the situation became difficult as I did not have the space or the required conditions at home. On the other hand, other research centers with which we had collaboration agreements also canceled access, such is the case of the Optical Research Center, where we used the confocal microscope to detect subcellular location of proteins. The closure of institutions allowed me to write theoretical parts of my thesis, however, the experimental phase was definitely affected for at least six months. The experiment with the plants was almost completely lost. In the subsequent months I had the opportunity to re-enter my institution; however, under strict conditions and on staggered days, which made certain measurements that require daily continuity difficult. Currently, the laboratory is not as it used to look, full of colleagues sharing results and difficulties, exchanging advice and even certain materials. I think the pandemic has pushed us to do our work more individually and slowly. Consequently, my degree was delayed and transferred from 2020 to 2021. There are still many challenges to overcome, although activities have not been fully restored, science does not stop and we have found a way to face it, slowly but surely.</p>


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