scholarly journals Three dimensional dual labelled DNA fluorescent in situ hybridization analysis in fixed tissue sections

MethodsX ◽  
2014 ◽  
Vol 1 ◽  
pp. 30-35 ◽  
Author(s):  
Kristin D. Kernohan ◽  
Nathalie G. Bérubé
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Three Dimensional ◽  
Hybridization Analysis ◽  
Fixed Tissue ◽  
Tissue Sections

HCR of raphe serotonergic neurons in fixed tissue sections  v1

2021 ◽  
Author(s):  
Alex Buckley
Keyword(s):  
In Situ Hybridization ◽  
Brain Tissue ◽  
Mouse Brain ◽  
Fluorescent In Situ Hybridization ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Serotonergic Neurons ◽  
Fixed Tissue ◽  
Tissue Sections

This is an RNA fluorescent in-situ hybridization (FISH) protocol that utilizes hybridization chain reaction technology from Molecular Instruments. The protocol fluorescently labels different mRNAs (up to 4 different mRNAs) such that they become suitable for imaging. This protocol is designed specifically for fixed mouse brain tissue sections that contain raphe serotonergic neurons, but can be applied to other regions of the mouse brain as well.

scholarly journals P3.03-017 Fluorescent in situ Hybridization Analysis of MET Gene Status in Malignant Mesothelioma

2017 ◽  
Vol 12 (1) ◽  
pp. S1354
Author(s):  
Antonella Vigani ◽  
Sandra Salvi ◽  
Serena Varesano ◽  
Simona Boccardo ◽  
Paola Ferro ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Malignant Mesothelioma ◽  
Fluorescent In Situ Hybridization ◽  
Hybridization Analysis ◽  
Gene Status

scholarly journals Specific Detection of Lawsonia intracellularis in Porcine Proliferative Enteropathy Inferred from Fluorescent rRNA In Situ Hybridization

1998 ◽  
Vol 35 (2) ◽  
pp. 153-156 ◽  
Author(s):  
M. Boye ◽  
T. K. Jensen ◽  
K. Møller ◽  
T. D. Leser ◽  
S. E. Jorsal
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Lawsonia Intracellularis ◽  
Specific Detection ◽  
Fixed Tissue ◽  
Tissue Samples ◽  
Formalin Fixed Tissue ◽  
Proliferative Enteropathy ◽  
Formalin Fixed

Fluorescent in situ hybridization targeting 16S ribosomal RNA was used for specific detection of the obligate intracellular bacterium Lawsonia intracellularis in enterocytes from pigs affected by proliferative enteropathy. A specific oligonucleotide probe was designed and the specificity of the probe was determined by simultaneous comparison with indirect immunofluorescence assay for detection of L. intracellularis in formalin-fixed tissue samples from 15 pigs affected by porcine proliferative enteropathy. We used 10 tissue samples from pigs without proliferative mucosal changes as negative controls. The results showed that the oligonucleotide probe is specific for L. intracellularis and that fluorescent in situ hybridization targeting ribosomal RNA is a suitable and fast method for specific detection and histological recognition of L. intracellularis in formalin-fixed tissue.

Isolated cutaneous extramedullary relapse of leukemia confirmed by fluorescent in situ hybridization analysis

2012 ◽  
Vol 67 (4) ◽  
pp. e162-e164 ◽  
Author(s):  
Ben Friedman ◽  
Anokhi Jambusaria ◽  
Michi Shinohara ◽  
Rosalie Elenitsas ◽  
Noelle Frey ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Hybridization Analysis ◽  
Extramedullary Relapse
Sign in / Sign up

Export Citation Format

Share Document