Helicobacter pylori and Schistosoma japonicum co-infection in a Chinese population: helminth infection alters humoral responses to H. pylori and serum pepsinogen I/II ratio

The study aimed to determine the association between cytotoxin-associated gene product (CagA), serum gastrin and pepsinogen levels in Japanese children infected with Helicobacter pylori. Three hundred children were enrolled in the study. H. pylori infection was assessed using an enzyme-linked immunosorbent assay, and CagA status was assessed using immunoblotting. Serum gastrin and pepsinogen concentrations were measured by radioimmunoassay. H. pylori seroprevalence was 12.3% (37/300) and CagA status was identified in 28/37 H. pylori-seropositive children (75.7%). Serum pepsinogen I and II levels were significantly higher in CagA-seropositive than CagA-seronegative children with H. pylori infection. There was no significant relationship between CagA seropositivity and serum gastrin levels. In conclusion, CagA status has a significant impact on serum pepsinogen levels, possibly through enhanced gastric mucosal inflammation.

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Diversity of 3′ variable region of cagA gene in Helicobacter pylori strains isolated from Chinese population

Gut Pathogens ◽

10.1186/s13099-021-00419-3 ◽

2021 ◽

Vol 13 (1) ◽

Author(s):

Zhijing Xue ◽

Yuanhai You ◽

Lihua He ◽

Yanan Gong ◽

Lu Sun ◽

...

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Amino Acid ◽

Chinese Population ◽

Statistical Difference ◽

Variable Region ◽

Repeat Region ◽

Geographical Regions ◽

H Pylori ◽

Epiya Motifs

Abstract Background The cytotoxin-associated gene A (cagA) is one of the most important virulence factors of Helicobacter pylori (H. pylori). There is a highly polymorphic Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region in the C-terminal of CagA protein. This repeat region is thought to play an important role in the pathogenesis of gastrointestinal diseases. The aim of this study was to investigate the diversity of cagA 3′ variable region and the amino acid polymorphisms in the EPIYA segments of the CagA C-terminal region of H. pylori, and their association with gastroduodenal diseases. Methods A total of 515 H. pylori strains from patients in 14 different geographical regions of China were collected. The genomic DNA from each strain was extracted and the cagA 3′ variable region was amplified by polymerase chain reaction (PCR). The PCR products were sequenced and analyzed using MEGA 7.0 software. Results A total of 503 (97.7%) H. pylori strains were cagA-positive and 1,587 EPIYA motifs were identified, including 12 types of EPIYA or EPIYA-like sequences. In addition to the four reported major segments, several rare segments (e.g., B′, B″ and D′) were defined and 20 different sequence types (e.g., ABD, ABC) were found in our study. A total of 481 (95.6%) strains carried the East Asian type CagA, and the ABD subtypes were most prevalent (82.1%). Only 22 strains carried the Western type CagA, which included AC, ABC, ABCC and ABCCCC subtypes. The CagA-ABD subtype had statistical difference in different geographical regions (P = 0.006). There were seven amino acid polymorphisms in the sequences surrounding the EPIYA motifs, among which amino acids 893 and 894 had a statistical difference with gastric cancer (P = 0.004). Conclusions In this study, 503 CagA sequences were studied and analyzed in depth. In Chinese population, most H. pylori strains were of the CagA-ABD subtype and its presence was associated with gastroduodenal diseases. Amino acid polymorphisms at residues 893 and 894 flanking the EPIYA motifs had a statistically significant association with gastric cancer.

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Diversity of 3’ variable region of cagA gene in Helicobacter pylori strains isolated from Chinese population

10.21203/rs.3.rs-127429/v2 ◽

2021 ◽

Author(s):

Zhijing Xue ◽

Yuanhai You ◽

Lihua He ◽

Yanan Gong ◽

Lu Sun ◽

...

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Amino Acid ◽

Chinese Population ◽

Statistical Difference ◽

Variable Region ◽

Repeat Region ◽

Geographical Regions ◽

H Pylori ◽

Epiya Motifs

Abstract Background: The cytotoxin-associated gene A (cagA) is one of the most important virulence factors of Helicobacter pylori (H. pylori). There is a highly polymorphic Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region in the C-terminal of CagA protein. This repeat region is thought to play an important role in the pathogenesis of gastrointestinal diseases. The aim of this study was to investigate the diversity of cagA 3’ variable region and the amino acid polymorphisms in the EPIYA segments of the CagA C-terminal region of H. pylori, and their association with gastroduodenal diseases.Methods: A total of 515 H. pylori strains from patients in 14 different geographical regions of China were collected. The genomic DNA from each strain was extracted and the cagA 3’ variable region was amplified by polymerase chain reaction (PCR). The PCR products were sequenced and analyzed using MEGA 7.0 software.Results: A total of 503 (97.7%) H. pylori strains were cagA-positive and 1,587 EPIYA motifs were identified, including 12 types of EPIYA or EPIYA-like sequences. In addition to the four reported major segments, several rare segments (e.g., B’, B’’ and D’) were defined and 20 different sequence types (e.g., ABD, ABC) were found in our study. A total of 481 (95.6%) strains carried the East Asian type CagA, and the ABD subtypes were most prevalent (82.1%). Only 22 strains carried the Western type CagA, which include AC, ABC, ABCC and ABCCCC subtypes. The CagA-ABD subtype had statistical difference in different geographic regions (P = 0.006). There are seven amino acid polymorphisms in the sequences surrounding the EPIYA motifs, among which amino acid residue 893 and 894 had a statistical difference with gastric cancer (P = 0.004).Conclusions: In this study, 503 CagA sequences was studied and analyzed in depth. In Chinese population, most H. pylori strains are of the CagA-ABD subtype and its presence was associated with gastroduodenal diseases. Amino acid polymorphisms at residue 893 and 894 flanking the EPIYA motif had a statistically significant association with gastric cancer.

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Su1248 – Combination of Helicobacter Pylori Status and Serum Pepsinogen I/Ii Ratio and Olga/Olgim Staging For Prediction of Gastric Cancer and Dysplasia in South Korea

Gastroenterology ◽

10.1016/s0016-5085(19)38174-0 ◽

2019 ◽

Vol 156 (6) ◽

pp. S-518-S-519

Author(s):

Nayoung Kim ◽

Sung Min Baek ◽

Young Jae Kwon ◽

Dong Ho Lee

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

South Korea ◽

Serum Pepsinogen ◽

Pepsinogen I

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Effect of Helicobacter pylori on Serum Pepsinogen I and Plasma Gastrin in Duodenal Ulcer Patients

Scandinavian Journal of Gastroenterology ◽

10.3109/00365529209011160 ◽

1992 ◽

Vol 27 (1) ◽

pp. 20-24 ◽

Cited By ~ 35

Author(s):

R. S. Chittajallu ◽

C. A. Dorrian ◽

J. E. S. Ardill ◽

K. E. L. McColl

Keyword(s):

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Serum Pepsinogen ◽

Plasma Gastrin ◽

Pepsinogen I

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Serum pepsinogen I and II concentrations and IgG antibody to Helicobacter pylori in dyspeptic patients.

Journal of Clinical Pathology ◽

10.1136/jcp.46.9.826 ◽

1993 ◽

Vol 46 (9) ◽

pp. 826-828 ◽

Cited By ~ 17

Author(s):

G Biasco ◽

G M Paganelli ◽

D Vaira ◽

J Holton ◽

G Di Febo ◽

...

Keyword(s):

Helicobacter Pylori ◽

Serum Pepsinogen ◽

Igg Antibody ◽

Pepsinogen I

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Mo1571 Risk Stratification for Gastric Cancer Using Serum Pepsinogen and Helicobacter pylori Serology in a Chinese Population

Gastroenterology ◽

10.1016/s0016-5085(12)62426-3 ◽

2012 ◽

Vol 142 (5) ◽

pp. S-631 ◽

Cited By ~ 1

Author(s):

Yip C. Benjamin ◽

Zhu Feng ◽

Khek-Yu Ho ◽

Khor Christopher ◽

Lee Guan Lim ◽

...

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Risk Stratification ◽

Chinese Population ◽

Serum Pepsinogen

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Is There an Association between Variceal Bleed and Helicobacter pylori Infection in Cirrhotic Patients with Portal Hypertension?

Sultan Qaboos University Medical Journal [SQUMJ] ◽

10.18295/squmj.9.2021.136 ◽

2021 ◽

Author(s):

Sathyanarayan Varuna ◽

Sathasivam Sureshkumar ◽

Balakrishnan Gurushankari ◽

Elangovan Archana ◽

Subair Mohsina ◽

...

Keyword(s):

Helicobacter Pylori ◽

Portal Hypertension ◽

Liver Disease ◽

Esophageal Varices ◽

Lower Risk ◽

Acid Output ◽

Gastric Acid Output ◽

Pepsinogen I ◽

H Pylori ◽

Related Liver Disease

Objectives: The objective of this study was to find the association of H. pylori in patients with variceal bleeding as well as rebleeding in cases of cirrhosis with portal hypertension. Methods: This was a prospective cohort of patients with bleeding esophageal varices. The primary outcome was correlation between prevalence of H. pylori and the incidence of bleeding/ rebleeding from varices and with encephalopathy. The secondary outcome were correlation between the site of bleeding with H. pylori infection and the association of pepsinogen I & II and the ratio of pepsinogen I/II with bleeding. Results: A total of 190 patients were assessed for eligibility, out of which 159 patients were included in this study. 124 out of 159 patients (77.9%) had alcohol-related liver disease. 8 out of 159 patients had HBV-related liver disease. 7 patients with varices had neither bled at presentation nor did bleed in the follow-up period. A total of 78 out of 159 (49.05%) patients were H.pylori-infected. Patients with esophageal varices [Adjusted Risk (AR)=0.7] and H.pylori infection (AR=0.7) had a lower risk of variceal rebleeding. Among the patients negative for H.pylori, pepsinogen I was higher in patients with rebleeding (30.7 vs 14.4; p<0.001). Among H.pylori positive patients, the ratio of pepsinogen I/II was higher in patients with rebleeding (2.9 vs 1.3; p=0.023). Conclusion: H.pylori infection was associated with a lower risk of rebleeding in cases of cirrhosis with portal hypertension. Irrespective of the status of H.pylori infection, rebleeding was associated with more gastric acid output demonstrated by the level of pepsinogen. Keywords: Pepsinogen; hepatic encephalopathy; gastric acid output, Helicobacter pylori

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Diversity of 3’ Variable Region of cagA Gene in Helicobacter Pylori Strains Isolated from Chinese Population

10.21203/rs.3.rs-127429/v1 ◽

2020 ◽

Author(s):

zhijing Xue ◽

Yuanhai You ◽

Lihua He ◽

Yanan Gong ◽

Lu Sun ◽

...

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Amino Acid ◽

Chinese Population ◽

Statistical Difference ◽

Variable Region ◽

Repeat Region ◽

Geographical Regions ◽

H Pylori ◽

Epiya Motifs

Abstract Background: CagA is one of the most important virulence factors of Helicobacter pylori (H. pylori). There is a highly polymorphic Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region in the CagA 3’ variable region. This repeat region is thought to play an important role in the pathogenesis of gastrointestinal diseases. The aim of this study was to investigate the diversity of CagA 3’ variable region and the amino acid polymorphisms in the EPIYA segments, and their association with gastroduodenal diseases.Methods: A total of 515 H. pylori isolates from patients in 14 different geographical regions of China were collected and the genomic DNA was extracted. The 3’ variable region of the cagA was amplified by polymerase chain reaction (PCR) and then followed by DNA sequencing, and the amino acid sequences were analyzed with MEGA 7.0 software.Results: A total of 503 (97.7%) H. pylori isolates were cagA-positive and 1,587 EPIYA motifs were obtained, including 12 types of EPIYA or EPIYA-like sequences. In addition to the four reported major segments, several rare segments (e.g., B’, B’’ and D’) were defined and 20 different sequence types (e.g., ABD, ABC) were found in our study. A total of 481 (95.6%) strains were East Asian type, most of them were ABD subtype (82.1%). Only 22 strains were Western type, including types AC, ABC, ABCC and ABCCCC. The CagA-ABD subtype had statistical difference in different geographic regions (P=0.006). There are seven amino acid polymorphisms in the sequences surrounding the EPIYA motifs, among which amino acid residue 893 and 894 had a statistical difference with gastric cancer (P=0.004).Conclusions: In this study, 503 CagA sequences was studied and analyzed in depth. In Chinese population, most H. pylori isolates are of the CagA-ABD subtype and its presence was associated with gastroduodenal disease. Amino acid polymorphisms at residue 893 and 894 flanking the EPIYA motif had a statistically significant association with gastric cancer.

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Association of Helicobacter pylori Infection and Atrophic Gastritis with Chronic Renal Insufficiency in Yaounde Cameroon, Using Gastropanel® Serological Biomarker Panel (Pepsinogen I; Pepsinogen II; Gastrin-17; Helicobacter pylori Igg)

Journal of Advances in Microbiology ◽

10.9734/jamb/2021/v21i230328 ◽

2021 ◽

pp. 62-67

Author(s):

Alonge Ivo Ebule ◽

Valentine Ngum Ndze ◽

Ngouana Kammalac Thierry ◽

Guenou Etienne ◽

Moche Mboudja Morel Ornella ◽

...

Keyword(s):

Helicobacter Pylori ◽

Atrophic Gastritis ◽

Teaching Hospital ◽

Helicobacter Pylori Infection ◽

University Teaching ◽

Renal Diseases ◽

Pepsinogen I ◽

Software Application ◽

The Mean ◽

H Pylori

Introduction: Helicobacter pylori infection is associated with an atrophic gastritis peptic and duodenal ulcer and gastric cancer. Patients with chronic renal diseases usually have dyspeptic symptoms. Several investigations have demonstrated an association between H. pylori infection and chronic kidney disease, although their results are still conflicting. We therefore aimed, to clarify the prevalence of H. pylori infection in patients receiving dialysis. Materials and Methods: Patients undergoing hemodialysis were recruited at the University Teaching Hospital of Yaounde, between January and May 2019. The clinical and socio-demographic information of the patients was recorded. 5 ml of blood were collected aseptically for Pepsinogen I and II enzymes, gastrin17 hormone and IgG anti H. pylori anti-body. The test parameters were analyzed using a GastroSoft software application. The data was analyzed using Epi Info 7.0. All statistics were 95% CI. Ethical clearance was also obtained from the National Ethics Committee. Authorization was obtained atthe University Teaching Hospital. Results: A total of 60 subjects were recruited aged 25-74 years, (mean±SD 52.03 ± 12,78) years;22(45.16%) females, aged 29 to 71years(mean±SD 47,45 ± 11.46) years and 38(54.84%) males aged 25 to 74 (mean±SD 56,47±12.25) years. Female / male ratio was 1.2. Overall, 26(43.33%) subjects were positive for H. pylori infection (IgG≥30EIU). The prevalence of atrophic gastritis obtained was (23.33%)(PG1< 30µg/l). The mean H. pylori IgG antibodies were significantly higher in obese than non obese subjects (F=3.59; p=0,01). A significant increase in the mean creatinine(P=0.008), andurea (P=0,05) was observed in H. pylori positive than negative ones. Conclusion: H. pylori infection is highly prevalent amongst patients with chronic renal failure and may thus require continuous follow up.

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