Pentavalent outer membrane vesicles of Vibrio cholerae induce adaptive immune response and protective efficacy in both adult and passive suckling mice models

2015 ◽  
Vol 17 (3) ◽  
pp. 215-227 ◽  
Author(s):  
Ritam Sinha ◽  
Hemanta Koley ◽  
Dhrubajyoti Nag ◽  
Soma Mitra ◽  
Asish K. Mukhopadhyay ◽  
...  
Keyword(s):  
Immune Response ◽  
Outer Membrane ◽  
Vibrio Cholerae ◽  
Protective Efficacy ◽  
Adaptive Immune Response ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Suckling Mice ◽  
Adaptive Immune

scholarly journals Immunogenicity and protective efficacy of Vibrio cholerae  outer membrane vesicles in rabbit model

2010 ◽  
Vol 60 (1) ◽  
pp. 18-27 ◽  
Author(s):  
Nivedita Roy ◽  
Soumik Barman ◽  
Amit Ghosh ◽  
Amit Pal ◽  
Krishnendu Chakraborty ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Vibrio Cholerae ◽  
Protective Efficacy ◽  
Rabbit Model ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles

scholarly journals Characterization of Vibrio cholerae Outer Membrane Vesicles as a Candidate Vaccine for Cholera

2008 ◽  
Vol 77 (1) ◽  
pp. 472-484 ◽  
Author(s):  
Stefan Schild ◽  
Eric J. Nelson ◽  
Anne L. Bishop ◽  
Andrew Camilli
Keyword(s):  
Immune Response ◽  
Outer Membrane ◽  
Vibrio Cholerae ◽  
Stomach Contents ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Antigen Delivery ◽  
Heterologous Antigen ◽  
Adult Mice ◽  
Delivery Vehicles

ABSTRACT Outer membrane vesicles (OMVs) offer a new approach for an effective cholera vaccine. We recently demonstrated that immunization of female mice with OMVs induces a long-lasting immune response and results in protection of their neonatal offspring from Vibrio cholerae intestinal colonization. This study investigates the induced protective immunity observed after immunization with OMVs in more detail. Analysis of the stomach contents and sera of the neonates revealed significant amounts of anti-OMV immunoglobulins (Igs). Swapping of litters born to immunized and nonvaccinated control mice allowed us to distinguish between prenatal and neonatal uptakes of Igs. Transfer of Igs to neonates via milk was sufficient for complete protection of the neonates from colonization with V. cholerae, while prenatal transfer alone reduced colonization only. Detection of IgA and IgG1 in the fecal pellets of intranasally immunized adult mice indicates an induced immune response at the mucosal surface in the gastrointestinal tract, which is the site of colonization by V. cholerae. When a protocol with three intranasal immunizations 14 days apart was used, the OMVs proved to be efficacious at doses as low as 0.025 μg per immunization. This is almost equivalent to OMV concentrations found naturally in the supernatants of LB-grown cultures of V. cholerae. Heterologous expression of the periplasmic alkaline phosphatase (PhoA) of Escherichia coli resulted in the incorporation of PhoA into OMVs derived from V. cholerae. Intranasal immunization with OMVs loaded with PhoA induced a specific immune response against this heterologous antigen in mice. The detection of an immune response against this heterologously expressed protein is a promising step toward the potential use of OMVs as antigen delivery vehicles in vaccine design.

scholarly journals Cholera Toxin Encapsulated within Several Vibrio cholerae O1 Serotype Inaba Outer Membrane Vesicles Lacks a Functional B-Subunit

Toxins ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 207 ◽  
Author(s):  
Elnaz Rasti ◽  
Angela Brown
Keyword(s):  
Outer Membrane ◽  
Cholera Toxin ◽  
Vibrio Cholerae ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Biologically Active ◽  
Host Cells ◽  
Free Form ◽  
Type Ii Secretion ◽  
Secondary Mechanism

Cholera toxin (CT), the major virulence factor of Vibrio cholerae, is an AB5 toxin secreted through the type II secretion system (T2SS). Upon secretion, the toxin initiates endocytosis through the interaction of the B pentamer with the GM1 ganglioside receptor on small intestinal cells. In addition to the release of CT in the free form, the bacteria secrete CT in association with outer membrane vesicles (OMVs). Previously, we demonstrated that strain 569B releases OMVs that encapsulate CT and which interact with host cells in a GM1-independent mechanism. Here, we have demonstrated that OMV-encapsulated CT, while biologically active, does not exist in an AB5 form; rather, the OMVs encapsulate two enzymatic A-subunit (CTA) polypeptides. We further investigated the assembly and secretion of the periplasmic CT and found that a major fraction of periplasmic CTA does not participate in the CT assembly process and instead is continuously encapsulated within the OMVs. Additionally, we found that the encapsulation of CTA fragments in OMVs is conserved among several Inaba O1 strains. We further found that under conditions in which the amount of extracellularly secreted CT increases, the concentration of OMV-encapsulated likewise CTA increases. These results point to a secondary mechanism for the secretion of biologically active CT that does not depend on the CTB-GM1 interaction for endocytosis.

scholarly journals Naturally Occurring IgG Antibodies Provide Innate Protection against Vibrio cholerae Bacteremia by Recognition of the Outer Membrane Protein U

2016 ◽  
Vol 8 (3) ◽  
pp. 269-283 ◽  
Author(s):  
Kyaw Min Aung ◽  
Annika E. Sjöström ◽  
Ulrich von Pawel-Rammingen ◽  
Kristian Riesbeck ◽  
Bernt Eric Uhlin ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Vibrio Cholerae ◽  
Outer Membrane Protein ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Immune Defense ◽  
Dependent Manner ◽  
Naturally Occurring ◽  
Serum Killing

Cholera epidemics are caused by Vibrio cholerae serogroups O1 and O139, whereas strains collectively known as non-O1/non-O139 V. cholerae are found in cases of extraintestinal infections and bacteremia. The mechanisms and factors influencing the occurrence of bacteremia and survival of V. cholerae in normal human serum have remained unclear. We found that naturally occurring IgG recognizing V. cholerae outer membrane protein U (OmpU) mediates a serum-killing effect in a complement C1q-dependent manner. Moreover, outer membrane vesicles (OMVs) containing OmpU caused enhanced survival of highly serum-sensitive classical V. cholerae in a dose-dependent manner. OMVs from wild-type and ompU mutant V. cholerae thereby provided a novel means to verify by extracellular transcomplementation the involvement of OmpU. Our data conclusively indicate that loss, or reduced expression, of OmpU imparts resistance to V. cholerae towards serum killing. We propose that the difference in OmpU protein levels is a plausible reason for differences in serum resistance and the ability to cause bacteremia observed among V. cholerae biotypes. Our findings provide a new perspective on how naturally occurring antibodies, perhaps induced by members of the microbiome, may play a role in the recognition of pathogens and the provocation of innate immune defense against bacteremia.

scholarly journals Expression of Heterologous Antigens in Commensal Neisseria spp.: Preservation of Conformational Epitopes with Vaccine Potential

2004 ◽  
Vol 72 (11) ◽  
pp. 6511-6518 ◽  
Author(s):  
Clíona A. O'Dwyer ◽  
Karen Reddin ◽  
Denis Martin ◽  
Stephen C. Taylor ◽  
Andrew R. Gorringe ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Meningococcal Disease ◽  
Protective Efficacy ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Heterologous Proteins ◽  
Protein Vaccine ◽  
Native Conformation ◽  
Protein Antigens

ABSTRACT Commensal neisseriae share with Neisseria meningitidis (meningococcus) a tendency towards overproduction of the bacterial outer envelope, leading to the formation and release during growth of outer membrane vesicles (OMVs). OMVs from both meningococci and commensal neisseriae have shown promise as vaccines to protect against meningococcal disease. We report here the successful expression at high levels of heterologous proteins in commensal neisseriae and the display, in its native conformation, of one meningococcal outer membrane protein vaccine candidate, NspA, in OMVs prepared from such a recombinant Neisseria flavescens strain. These NspA-containing OMVs conferred protection against otherwise lethal intraperitoneal challenge of mice with N. meningitidis serogroup B, and sera raised against them mediated opsonophagocytosis of meningococcal strains expressing this antigen. This development promises to facilitate the design of novel vaccines containing membrane protein antigens that are otherwise difficult to present in native conformation that provide cross-protective efficacy in the prevention of meningococcal disease.

scholarly journals Immunization of Mice With Vibrio cholerae Outer-Membrane Vesicles Protects Against Hyperinfectious Challenge and Blocks Transmission

2011 ◽  
Vol 205 (3) ◽  
pp. 412-421 ◽  
Author(s):  
Anne L. Bishop ◽  
Abdullah A. Tarique ◽  
Bharathi Patimalla ◽  
Stephen B. Calderwood ◽  
Firdausi Qadri ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Vibrio Cholerae ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles

scholarly journals Bacterial Outer Membrane Vesicles Induce Plant Immune Responses

2016 ◽  
Vol 29 (5) ◽  
pp. 374-384 ◽  
Author(s):  
Ofir Bahar ◽  
Gideon Mordukhovich ◽  
Dee Dee Luu ◽  
Benjamin Schwessinger ◽  
Arsalan Daudi ◽  
...  
Keyword(s):  
Immune Response ◽  
Outer Membrane ◽  
Plant Pathogens ◽  
Cell Communication ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Defense Gene Expression ◽  
Defense Gene ◽  
Bacterial Communication ◽  
Multiple Processes

Gram-negative bacteria continuously pinch off portions of their outer membrane, releasing membrane vesicles. These outer membrane vesicles (OMVs) are involved in multiple processes including cell-to-cell communication, biofilm formation, stress tolerance, horizontal gene transfer, and virulence. OMVs are also known modulators of the mammalian immune response. Despite the well-documented role of OMVs in mammalian-bacterial communication, their interaction with plants is not well studied. To examine whether OMVs of plant pathogens modulate the plant immune response, we purified OMVs from four different plant pathogens and used them to treat Arabidopsis thaliana. OMVs rapidly induced a reactive oxygen species burst, medium alkalinization, and defense gene expression in A. thaliana leaf discs, cell cultures, and seedlings, respectively. Western blot analysis revealed that EF-Tu is present in OMVs and that it serves as an elicitor of the plant immune response in this form. Our results further show that the immune coreceptors BAK1 and SOBIR1 mediate OMV perception and response. Taken together, our results demonstrate that plants can detect and respond to OMV-associated molecules by activation of their immune system, revealing a new facet of plant-bacterial interactions.

scholarly journals Outer membrane vesicles blebbing contributes to B. vulgatus mpk-mediated immune response silencing

Gut Microbes ◽  
2017 ◽  
Vol 9 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Jan Kevin Maerz ◽  
Alex Steimle ◽  
Anna Lange ◽  
Annika Bender ◽  
Birgit Fehrenbacher ◽  
...  
Keyword(s):  
Immune Response ◽  
Outer Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles
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