Regulation of the Vibrio vulnificus vvpE expression by cyclic AMP-receptor protein and quorum-sensing regulator SmcR

2010 ◽  
Vol 49 (6) ◽  
pp. 348-353 ◽  
Author(s):  
Choon-Mee Kim ◽  
Sung-Heui Shin
Keyword(s):  
Quorum Sensing ◽  
Cyclic Amp ◽  
Vibrio Vulnificus ◽  
Receptor Protein ◽  
Cyclic Amp Receptor Protein

Cyclic AMP-receptor protein activates aerobactin receptor IutA expression in Vibrio vulnificus

2012 ◽  
Vol 50 (2) ◽  
pp. 320-325 ◽  
Author(s):  
Choon-Mee Kim ◽  
Seong-Jung Kim ◽  
Sung-Heui Shin
Keyword(s):  
Cyclic Amp ◽  
Vibrio Vulnificus ◽  
Receptor Protein ◽  
Cyclic Amp Receptor Protein

scholarly journals Regulation of the Vibrio vulnificus hupA Gene by Temperature Alteration and Cyclic AMP Receptor Protein and Evaluation of Its Role in Virulence

2009 ◽  
Vol 77 (3) ◽  
pp. 1208-1215 ◽  
Author(s):  
Man Hwan Oh ◽  
Sung Min Lee ◽  
Dong Hwan Lee ◽  
Sang Ho Choi
Keyword(s):  
Cyclic Amp ◽  
Pathogenic Bacteria ◽  
Vibrio Vulnificus ◽  
Receptor Protein ◽  
Iron Regulation ◽  
Mammalian Host ◽  
Transcriptional Level ◽  
Iron Availability ◽  
Cyclic Amp Receptor Protein ◽  
Successful Infection

ABSTRACT Availability of free iron is extremely limited in the mammalian host, and the acquisition of iron in the host is essential for successful infection by pathogenic bacteria. Expression of many genes involved in acquiring iron is regulated in response to the level of iron availability, and iron regulation is mediated by Fur. In this study, cellular levels of Vibrio vulnificus HupA, a heme receptor protein, and the hupA transcript were found to increase in cells grown at 40°C compared to cells grown at 30°C. The results suggested that change in growth temperature, in addition to iron availability, is an environmental cue controlling the expression of the hupA gene. The influence of global regulatory proteins on the expression of hupA was examined, and the cyclic AMP receptor protein (CRP) was found to activate the expression of hupA at the transcriptional level. CRP exerts its effects by directly binding to DNA upstream of the hupA promoter P hupA , and a CRP binding site, centered at 174 bp upstream of the transcription start site, was identified by a DNase I protection assay. Finally, a hupA mutant showed reduced virulence in mice and in tissue cultures, in which growth of the hupA mutant was impaired, indicating that HupA of V. vulnificus is essential for survival and multiplication during infection.

Temperature Change Induces the Expression of vuuA Encoding Vulnibactin Receptor and crp Encoding Cyclic AMP Receptor Protein in Vibrio vulnificus

2016 ◽  
Vol 73 (1) ◽  
pp. 54-64 ◽  
Author(s):  
Choon-Mee Kim ◽  
Young-Joon Ahn ◽  
Seong-Jung Kim ◽  
Dae-Heung Yoon ◽  
Sung-Heui Shin
Keyword(s):  
Cyclic Amp ◽  
Temperature Change ◽  
Vibrio Vulnificus ◽  
Receptor Protein ◽  
Cyclic Amp Receptor Protein

scholarly journals Evidence that Expression of the Vibrio vulnificus Hemolysin Gene Is Dependent on Cyclic AMP and Cyclic AMP Receptor Protein

1999 ◽  
Vol 181 (24) ◽  
pp. 7639-7642 ◽  
Author(s):  
Young Bae Bang ◽  
Shee Eun Lee ◽  
Joon Haeng Rhee ◽  
Sang Ho Choi
Keyword(s):  
Escherichia Coli ◽  
Cyclic Amp ◽  
Promoter Activity ◽  
Vibrio Vulnificus ◽  
Receptor Protein ◽  
Camp Receptor Protein ◽  
Transcriptional Fusion ◽  
Cyclic Amp Receptor Protein ◽  
Hemolysin Gene ◽  
Camp Receptor

ABSTRACT Glucose repressed hemolysin production in Vibrio vulnificus. Promoter activity of the hemolysin gene,vvh, assessed with a vvh-luxCDABEtranscriptional fusion, required cyclic AMP (cAMP) and cAMP receptor protein (CRP) in Escherichia coli. Hemolysin production inV. vulnificus increased after the addition of cAMP and was undetectable in a putative crp mutant, suggesting thatvvh is also regulated by cAMP-CRP in V. vulnificus.

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