Cyclic AMP and Cyclic AMP-receptor Protein Modulate the Autoinducer-2-Mediated Quorum Sensing System in Vibrio vulnificus

2012 ◽  
Vol 65 (6) ◽  
pp. 701-710 ◽  
Author(s):  
Sun-Pyo Kim ◽  
Choon-Mee Kim ◽  
Sung-Heui Shin
Keyword(s):  
Quorum Sensing ◽  
Cyclic Amp ◽  
Vibrio Vulnificus ◽  
Receptor Protein ◽  
Cyclic Amp Receptor Protein ◽  
Sensing System ◽  
Autoinducer 2 ◽  
Quorum Sensing System

scholarly journals Expression of a luxS Gene Is Not Required for Borrelia burgdorferi Infection of Mice via Needle Inoculation

2003 ◽  
Vol 71 (5) ◽  
pp. 2892-2896 ◽  
Author(s):  
Anette Hübner ◽  
Andrew T. Revel ◽  
Dena M. Nolen ◽  
Kayla E. Hagman ◽  
Michael V. Norgard
Keyword(s):  
Quorum Sensing ◽  
Borrelia Burgdorferi ◽  
Mammalian Host ◽  
Wild Type ◽  
Sensing System ◽  
Autoinducer 2 ◽  
Sensing Systems ◽  
Quorum Sensing System ◽  
Culture Supernatants

ABSTRACT The luxS gene product is an integral component of LuxS/autoinducer-2 (AI-2) quorum-sensing systems in bacteria. A putative luxS gene was expressed at comparable levels by Borrelia burgdorferi strain 297 cultivated either in vitro or in dialysis membrane chambers implanted in rat peritoneal cavities. Although the borrelial luxS gene functionally complemented a LuxS deficiency in Escherichia coli DH5α, AI-2-like activity could not be detected within B. burgdorferi culture supernatants or concentrated cell lysates. Finally, a luxS-deficient mutant of B. burgdorferi was infectious at wild-type levels when it was intradermally needle inoculated into mice, indicating that expression of luxS probably is not required for infectivity but, at the very least, is not essential for mammalian host adaptation. Our findings also challenge the notion that a LuxS/AI-2 quorum-sensing system is operative in B. burgdorferi.

Cyclic AMP-receptor protein activates aerobactin receptor IutA expression in Vibrio vulnificus

2012 ◽  
Vol 50 (2) ◽  
pp. 320-325 ◽  
Author(s):  
Choon-Mee Kim ◽  
Seong-Jung Kim ◽  
Sung-Heui Shin
Keyword(s):  
Cyclic Amp ◽  
Vibrio Vulnificus ◽  
Receptor Protein ◽  
Cyclic Amp Receptor Protein

scholarly journals Role of the luxS Quorum-Sensing System in Biofilm Formation and Virulence of Staphylococcus epidermidis

2006 ◽  
Vol 74 (1) ◽  
pp. 488-496 ◽  
Author(s):  
Lin Xu ◽  
Hualin Li ◽  
Cuong Vuong ◽  
Viveka Vadyvaloo ◽  
Jianping Wang ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Mutant Strain ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Bacterial Pathogens ◽  
Sensing System ◽  
Autoinducer 2 ◽  
Enhanced Production ◽  
Quorum Sensing System ◽  
Biofilm Development

ABSTRACT Nosocomial infections caused by Staphylococcus epidermidis are characterized by biofilm formation on implanted medical devices. Quorum-sensing regulation plays a major role in the biofilm development of many bacterial pathogens. Here, we describe luxS, a quorum-sensing system in staphylococci that has a significant impact on biofilm development and virulence. We constructed an isogenic ΔluxS mutant strain of a biofilm-forming clinical isolate of S. epidermidis and demonstrated that luxS signaling is functional in S. epidermidis. The mutant strain showed increased biofilm formation in vitro and enhanced virulence in a rat model of biofilm-associated infection. Genetic complementation and addition of autoinducer 2-containing culture filtrate restored the wild-type phenotype, demonstrating that luxS repressed biofilm formation through a cell-cell signaling mechanism based on autoinducer 2 secretion. Enhanced production of the biofilm exopolysaccharide polysaccharide intercellular adhesin in the mutant strain is presumably the major cause of the observed phenotype. The agr quorum-sensing system has previously been shown to impact biofilm development and biofilm-associated infection in a way similar to that of luxS, although by regulation of different factors. Our study indicates a general scheme of quorum-sensing regulation of biofilm development in staphylococci, which contrasts with that observed in many other bacterial pathogens.

Signal-mediated cross-talk regulates stress adaptation in Vibrio species

Microbiology ◽  
2003 ◽  
Vol 149 (7) ◽  
pp. 1923-1933 ◽  
Author(s):  
Diane McDougald ◽  
Sujatha Srinivasan ◽  
Scott A. Rice ◽  
Staffan Kjelleberg
Keyword(s):  
Quorum Sensing ◽  
Cross Talk ◽  
Communication System ◽  
Communication Systems ◽  
Vibrio Vulnificus ◽  
Homoserine Lactone ◽  
Sensing System ◽  
Sensing Systems ◽  
Quorum Sensing System ◽  
Signalling Systems

Quorum sensing systems serve as a means of ‘census taking’ of conspecific and non-conspecific bacteria in the near vicinity. The acylated homoserine lactone (AHL) quorum sensing system has been proposed to be primarily an intra-specific communication system, while the AI-2 autoinducer signalling system is proposed to be an interspecific communication system. Here it is shown that AI-2-like signalling in two marine Vibrio species, Vibrio vulnificus and ‘Vibrio angustum’ S14, induces the core response phenotypes of starvation adaptation and stress resistance, and that a signal antagonist can competitively inhibit these phenotypes. Furthermore, the signals produced by a range of Vibrio species have the ability to induce these phenotypes in V. vulnificus and ‘V. angustum’ S14, indicating that, at least in Vibrio species, AI-2-like signalling systems function as interspecies communication systems capable of ‘cross-talk’ and of regulating environmentally relevant phenotypes.

Autoinducer‐2‐mediated quorum‐sensing system resists T4 phage infection in Escherichia coli

2021 ◽  
Author(s):  
Yafang Ding ◽  
Dongfang Zhang ◽  
Xiaoman Zhao ◽  
Wenzhang Tan ◽  
Xiaodan Zheng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Phage Infection ◽  
Sensing System ◽  
Autoinducer 2 ◽  
Quorum Sensing System ◽  
T4 Phage

scholarly journals Regulation of Vibrio vulnificus virulence by the LuxS quorum-sensing system

2003 ◽  
Vol 48 (6) ◽  
pp. 1647-1664 ◽  
Author(s):  
Soo Young Kim ◽  
Shee Eun Lee ◽  
Young Ran Kim ◽  
Choon Mee Kim ◽  
Phil Youl Ryu ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Vibrio Vulnificus ◽  
Sensing System ◽  
Quorum Sensing System
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