Effects of shiga toxin 2 on cellular regeneration mechanisms in primary and three-dimensional cultures of human renal tubular epithelial cells

2016 ◽  
Vol 99 ◽  
pp. 87-94 ◽  
Author(s):  
Laura B. Márquez ◽  
Alicia Araoz ◽  
Horacio A. Repetto ◽  
Fernando R. Ibarra ◽  
Claudia Silberstein
Keyword(s):  
Epithelial Cells ◽  
Shiga Toxin ◽  
Three Dimensional ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Shiga Toxin 2 ◽  
Cellular Regeneration ◽  
Renal Tubular

Cytotoxic effect of Shiga toxin-2 holotoxin and its B subunit on human renal tubular epithelial cells

2006 ◽  
Vol 8 (2) ◽  
pp. 410-419 ◽  
Author(s):  
Virginia Pistone Creydt ◽  
Claudia Silberstein ◽  
Elsa Zotta ◽  
Cristina Ibarra
Keyword(s):  
Epithelial Cells ◽  
Shiga Toxin ◽  
Cytotoxic Effect ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
B Subunit ◽  
Shiga Toxin 2 ◽  
Renal Tubular

scholarly journals Activation of β2-Adrenoceptor Prevents Shiga Toxin 2-Induced TNF-α Gene Transcription

2001 ◽  
Vol 12 (11) ◽  
pp. 2288-2299 ◽  
Author(s):  
AKIO NAKAMURA ◽  
EDWARD J. JOHNS ◽  
AKIRA IMAIZUMI ◽  
YUKISHIGE YANAGAWA ◽  
TAKAO KOHSAKA
Keyword(s):  
Protein Kinase ◽  
Epithelial Cells ◽  
Gene Transcription ◽  
Shiga Toxin ◽  
Promoter Activity ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Tnf Α ◽  
Shiga Toxin 2 ◽  
Renal Tubular

Abstract. Exposure of renal tubular epithelial cells to shiga toxin 2 (Stx-2) causes cytotoxicity, and the potency of this toxin is enhanced in the presence of tumor necrosis factor—α (TNF-α). It has been shown that Stx-2 induces TNF-α production and that activation of β2-adrenoceptors downregulates TNF-α. However, little is known about the signaling pathway by which β2-adrenoceptor agonists suppress the Stx-2—induced TNF-α gene transcription. The possible signaling components involved in this pathway were investigated. Human adenocarcinoma—derived renal tubular epithelial cells (ACHN) were exposed to Stx-2 in the presence or absence of a β2-adrenoceptor agonist. Mitogen-activated protein kinase (MAPK), activating protein—1 (AP-1), and nuclear factor—κB (NF-κB) were measured to evaluate the regulatory mechanisms involved in TNF-α gene transcription. Stx-2 (4 pg/ml) stimulated MAPK (p42/p44, p38) and AP-1 and increased TNF-α promoter activity by 2.4-fold. The increase in TNF-α was attenuated by both a p42/p44 inhibitor, PD098059 (10-6 M), and a p38 inhibitor, SB203580 (10-6 M), and AP-1—binding activity was inhibited by PD098059. Terbutaline (10-6 M to 10-8 M) suppressed MAPK (p42/p44, p38), NF-κB (p50, p65), and TNF-α promoter activity in a dose-dependent way that was prevented by the β2-adrenoceptor antagonist, ICI118,551. However, inhibition of MAPK (p42/p44) and TNF-α promoter activity was partially prevented by the cAMP-protein kinase (PKA) inhibitors, H-89 (5 × 10-6 M) and KT5720 (10-5 M), whereas the suppression of p38 MAPK or NF-κB (p50) was not blocked by these inhibitors. The suppression of NF-κB (p65) was completely overcome by H-89 or KT5720. In summary, the downregulation of TNF-α transcription by terbutaline was mediated by an inhibitory effect of β2-adrenoceptor activation on MAPK (p42/p44, p38) and NF-κB (p50/p65), which were exerted through a cAMP-PKA pathway and a cAMP-independent mechanism. It is likely that cAMP-PKA and MAPK (p42/p44, p38) may play a critical role in the regulation of the Stx-2—induced TNF-α transcription via β2-adrenoceptor activation.

scholarly journals Effects of Escherichia Coli Subtilase Cytotoxin and Shiga Toxin 2 on Primary Cultures of Human Renal Tubular Epithelial Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e87022 ◽  
Author(s):  
Laura B. Márquez ◽  
Natalia Velázquez ◽  
Horacio A. Repetto ◽  
Adrienne W. Paton ◽  
James C. Paton ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cells ◽  
Shiga Toxin ◽  
Primary Cultures ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Shiga Toxin 2 ◽  
Renal Tubular ◽  
Subtilase Cytotoxin

Eliglustat prevents Shiga toxin 2 cytotoxic effects in human renal tubular epithelial cells

2021 ◽  
Author(s):  
Daiana S. Sánchez ◽  
Lilian K. Fischer Sigel ◽  
Alejandro Balestracci ◽  
Cristina Ibarra ◽  
María M. Amaral ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Shiga Toxin ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Cytotoxic Effects ◽  
Shiga Toxin 2 ◽  
Renal Tubular

CYTOKINE EXPRESSION IN THE RENAL TUBULAR EPITHELIAL CELLS STIMULATED BY SHIGA TOXIN 2 OFESCHERICHIA COLIO157:H7

Renal Failure ◽  
2002 ◽  
Vol 24 (5) ◽  
pp. 567-575 ◽  
Author(s):  
Ji Eun Lee ◽  
Jung Sim Kim ◽  
In Hyung Choi ◽  
Manabu Tagawa ◽  
Takao Kohsaka ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Shiga Toxin ◽  
Cytokine Expression ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Shiga Toxin 2 ◽  
Renal Tubular
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