Diverse effects of different Akkermansia muciniphila genotypes on Brown adipose tissue inflammation and whitening in a high-fat-diet murine model

Abstract Background The study aimed to investigate the differences of different Akkermansia muciniphila (A.muciniphila) genotypes on metabolic protective effects in mice with high-fat diet and explore possible mechanisms. Methods Male C57BL/6 mice were randomly divided into 6 groups, including high-fat diet (HFD)+ A.muciniphila I/II/PBS group, normal chow diet (NCD)+A.muciniphila I/ II /PBS group, respectively. Dietary intervention and A.muciniphila gavage were performed simultaneously. Blood glucose and lipid metabolism, brown adipose morphology and activities, and intestinal barrier function were examined after the mice were sacrificed. Results A.muciniphila gavage improved the impaired glucose tolerance, hyperlipidemia and liver steatosis in HFD mice, and that A.muciniphila II was not as effective as A.muciniphila I. This phenomenon might be because A.muciniphila I intervention significantly inhibited brown adipose tissue whitening and inflammation induced by HFD, by repairing the intestinal barrier and relieving endotoxemia. A.muciniphila II did not display the same results as A.muciniphila I in HFD mice, but had stronger effects in the NCD mice. Conclusions This study mainly reveals the distinct functions of different A.muciniphila genotypes on diet-induced obesity, suggesting that different A.muciniphila genotypes may play inequitable roles in pathological conditions through distinct action pathways.

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Brown adipose tissue metabolism in ob/ob mice: effects of a high-fat diet and adrenalectomy

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1987.253.2.e149 ◽

1987 ◽

Vol 253 (2) ◽

pp. E149-E157

Author(s):

H. K. Kim ◽

D. R. Romsos

Keyword(s):

Adipose Tissue ◽

Brown Adipose Tissue ◽

High Fat Diet ◽

High Fat ◽

Adipose Tissue Metabolism ◽

High Carbohydrate ◽

Bat Mitochondria ◽

Norepinephrine Turnover ◽

Brown Adipose ◽

Thermogenic Capacity

Adrenalectomy prevents development of obesity in ob/ob mice fed high-carbohydrate stock diets partly by stimulating the low thermogenic capacity of their brown adipose tissue (BAT). Adrenalectomy, however, fails to prevent development of obesity in ob/ob mice fed a high-fat diet. Effects of adrenalectomy on BAT metabolism in ob/ob mice fed a high-fat diet were thus examined. ob/ob mice fed the high-fat diet developed gross obesity despite normal BAT metabolism, as assessed by rates of norepinephrine turnover in BAT, GDP binding to BAT mitochondria, and GDP-inhibitable, chloride-induced mitochondrial swelling. Adrenalectomy failed to arrest the development of obesity or to influence BAT metabolism in ob/ob mice fed the high-fat diet. Development of obesity in ob/ob mice fed a high-fat diet is not associated with low thermogenic capacity of BAT or with adrenal secretions, as it is in ob/ob mice fed high-carbohydrate stock diets.

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Uncoupling protein-1 deficiency promotes brown adipose tissue inflammation and ER stress

PLoS ONE ◽

10.1371/journal.pone.0205726 ◽

2018 ◽

Vol 13 (11) ◽

pp. e0205726 ◽

Cited By ~ 7

Author(s):

Laura M. Bond ◽

Maggie S. Burhans ◽

James M. Ntambi

Keyword(s):

Adipose Tissue ◽

Er Stress ◽

Brown Adipose Tissue ◽

Uncoupling Protein ◽

Adipose Tissue Inflammation ◽

Uncoupling Protein 1 ◽

Tissue Inflammation ◽

Brown Adipose

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Brown adipose tissue whitening leads to brown adipocyte death and adipose tissue inflammation

The Journal of Lipid Research ◽

10.1194/jlr.m079665 ◽

2018 ◽

Vol 59 (5) ◽

pp. 784-794 ◽

Cited By ~ 51

Author(s):

Petra Kotzbeck ◽

Antonio Giordano ◽

Eleonora Mondini ◽

Incoronata Murano ◽

Ilenia Severi ◽

...

Keyword(s):

Adipose Tissue ◽

Brown Adipose Tissue ◽

Brown Adipocyte ◽

Adipose Tissue Inflammation ◽

Tissue Inflammation ◽

Brown Adipose

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The novel non‐steroidal MR antagonist finerenone improves metabolic parameters in high‐fat diet‐fed mice and activates brown adipose tissue via AMPK‐ATGL pathway

The FASEB Journal ◽

10.1096/fj.202000164r ◽

2020 ◽

Vol 34 (9) ◽

pp. 12450-12465 ◽

Cited By ~ 1

Author(s):

Vincenzo Marzolla ◽

Alessandra Feraco ◽

Stefania Gorini ◽

Caterina Mammi ◽

Carmen Marrese ◽

...

Keyword(s):

Adipose Tissue ◽

Brown Adipose Tissue ◽

High Fat Diet ◽

Metabolic Parameters ◽

The Novel ◽

High Fat ◽

Brown Adipose

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Spred2 Regulates High Fat Diet-Induced Adipose Tissue Inflammation, and Metabolic Abnormalities in Mice

Frontiers in Immunology ◽

10.3389/fimmu.2019.00017 ◽

2019 ◽

Vol 10 ◽

Cited By ~ 5

Author(s):

Takahiro Ohkura ◽

Teizo Yoshimura ◽

Masayoshi Fujisawa ◽

Toshiaki Ohara ◽

Rie Marutani ◽

...

Keyword(s):

Adipose Tissue ◽

High Fat Diet ◽

Metabolic Abnormalities ◽

Adipose Tissue Inflammation ◽

High Fat ◽

Tissue Inflammation

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Defective trophic response of brown adipose tissue of myopathic hamsters

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1984.247.6.e800 ◽

1984 ◽

Vol 247 (6) ◽

pp. E800-E807

Author(s):

J. Triandafillou ◽

W. Hellenbrand ◽

J. Himms-Hagen

Keyword(s):

Adipose Tissue ◽

Muscular Dystrophy ◽

Brown Adipose Tissue ◽

Protein Content ◽

High Fat Diet ◽

Marked Reduction ◽

Short Photoperiod ◽

Normal Amount ◽

High Fat ◽

Brown Adipose

Hamsters with muscular dystrophy (BIO 14.6) have a smaller than normal amount of brown adipose tissue. Two stimuli that promote growth of brown adipose tissue in normal hamsters, short photoperiod and eating a high-fat diet, are here shown to be without effect on brown adipose tissue of myopathic hamsters. Cold-induced growth of brown adipose tissue occurs normally [Am. J. Physiol. 239 (Cell Physiol. 8): C18–C22, 1980]. There is a normal rate of turnover of norepinephrine in brown adipose tissue of the myopathic hamster but a failure of the tissue to hypertrophy in response to norepinephrine is unlikely since norepinephrine does not appear to mediate the trophic response [Am. J. Physiol. 247 (Endocrinol. Metab. 10): E793–E799, 1984]. Denervation results in a marked reduction in size (protein content) of brown adipose tissue of normal hamsters but has very little effect on the size of brown adipose tissue of myopathic hamsters. A central, possibly hypothalamic, defect in the myopathic hamster is postulated to underlie its abnormal control of brown adipose tissue hypertrophy.

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Anti-Obesity Effects of Grateloupia elliptica, a Red Seaweed, in Mice with High-Fat Diet-Induced Obesity via Suppression of Adipogenic Factors in White Adipose Tissue and Increased Thermogenic Factors in Brown Adipose Tissue

Nutrients ◽

10.3390/nu12020308 ◽

2020 ◽

Vol 12 (2) ◽

pp. 308 ◽

Cited By ~ 4

Author(s):

Hyo-Geun Lee ◽

Yu An Lu ◽

Xining Li ◽

Ji-Min Hyun ◽

Hyun-Soo Kim ◽

...

Keyword(s):

Adipose Tissue ◽

Brown Adipose Tissue ◽

White Adipose Tissue ◽

High Fat Diet ◽

Red Seaweed ◽

High Fat ◽

Adipose Tissues ◽

Ppar Γ ◽

Brown Adipose ◽

Treatment Of Obesity

Obesity is a serious metabolic syndrome characterized by high levels of cholesterol, lipids in the blood, and intracellular fat accumulation in adipose tissues. It is known that the suppression of adipogenic protein expression is an effective approach for the treatment of obesity, and regulates fatty acid storage and transportation in adipose tissues. The 60% ethanol extract of Grateloupia elliptica (GEE), a red seaweed from Jeju Island in Korea, was shown to exert anti-adipogenic activity in 3T3-L1 cells and in mice with high-fat diet (HFD)-induced obesity. GEE inhibited intracellular lipid accumulation in 3T3-L1 cells, and significantly reduced expression of adipogenic proteins. In vivo experiments indicated a significant reduction in body weight, as well as white adipose tissue (WAT) weight, including fatty liver, serum triglycerides, total cholesterol, and leptin contents. The expression of the adipogenic proteins, SREBP-1 and PPAR-γ, was significantly decreased by GEE, and the expression of the metabolic regulator protein was increased in WAT. The potential of GEE was shown in WAT, with the downregulation of PPAR-γ and C/EBP-α mRNA; in contrast, in brown adipose tissue (BAT), the thermogenic proteins were increased. Collectively, these research findings suggest the potential of GEE as an effective candidate for the treatment of obesity-related issues via functional foods or pharmaceutical agents.

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