Influences of non-IgE-mediated cow's milk protein allergy-associated gut microbial dysbiosis on regulatory T cell-mediated intestinal immune tolerance and homeostasis

2021 ◽  
pp. 105020
Author(s):  
Jinzhi Wang ◽  
Shuang Zheng ◽  
Xin Yang ◽  
Ben Huazeng ◽  
Qian Cheng
Keyword(s):  
T Cell ◽  
Immune Tolerance ◽  
Regulatory T Cell ◽  
Milk Protein ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Microbial Dysbiosis ◽  
Ige Mediated ◽  
Cow’S Milk Protein

scholarly journals Fecal Calprotectin and Eosinophil-Derived Neurotoxin in Children with Non-IgE-Mediated Cow’s Milk Protein Allergy

2021 ◽  
Vol 10 (8) ◽  
pp. 1595
Author(s):  
María Roca ◽  
Ester Donat ◽  
Ana Rodriguez Varela ◽  
Eva Carvajal ◽  
Francisco Cano ◽  
...  
Keyword(s):  
Milk Protein ◽  
Statistical Significance ◽  
Functional Gastrointestinal Disorders ◽  
Fecal Calprotectin ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Young Infants ◽  
Healthy Infants ◽  
Ige Mediated ◽  
Cow’S Milk Protein

Our aim is to assess the efficacy of fecal calprotectin (fCP) and fecal eosinophil-derived neurotoxin (fEDN) as diagnostic markers of cow’s milk protein allergy (CMPA) and for monitoring the infants’ response to a non-IgE mediated cow’s milk protein (CMP)-free diet. We prospectively recruited infants aged 0 to 9 months. Stool samples were taken from 30 infants with CMPA, 19 with mild functional gastrointestinal disorders, 28 healthy infants, and 28 children who presented mild infections. Despite the fact that levels of fCP and fEDN in CMPA infants were higher than in healthy infants at month 0, differences for both parameters did not reach statistical significance (p-value 0.119 and 0.506). After 1 month of an elimination diet, no statistically significant differences in fCP with basal levels were found (p-values 0.184) in the CMPA group. We found a high variability in the fCP and fEDN levels of young infants, and discrepancies in individual behavior of these markers after a CMP-free diet was started. It seems that neither fCP nor fEDN levels are helpful to discriminate between healthy infants and those with signs or symptoms related to non-IgE-mediated CMPA. Additionally, it is debatable if on an individual basis, fCP or fEDN levels could be used for clinical follow-up and dietary compliance monitoring. However, prospective studies with larger populations are needed to draw robust conclusions.

scholarly journals T cell response and cytokines production after allergen stimulation in children allergic to cow’s milk protein

2013 ◽  
Vol 3 (S3) ◽  
Author(s):  
F Angelini ◽  
V Pacciani ◽  
S Corrente ◽  
E Monteferrario ◽  
ML Romiti ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Response ◽  
Milk Protein ◽  
T Cell Response ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Cow’S Milk Protein

scholarly journals Peptide Release after Simulated Infant In Vitro Digestion of Dry Heated Cow’s Milk Protein and Transport of Potentially Immunoreactive Peptides across the Caco-2 Cell Monolayer

Nutrients ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2483 ◽  
Author(s):  
Hannah E. Zenker ◽  
Harry J. Wichers ◽  
Monic M. M. Tomassen ◽  
Sjef Boeren ◽  
Nicolette W. De Jong ◽  
...  
Keyword(s):  
T Cell ◽  
Milk Protein ◽  
Cell Monolayer ◽  
In Vitro Digestion ◽  
Cow’S Milk ◽  
T Cell Epitopes ◽  
Peptide Profile ◽  
Cow's Milk ◽  
Cow’S Milk Protein

Dry heating of cow’s milk protein, as applied in the production of “baked milk”, facilitates the resolution of cow’s milk allergy symptoms upon digestion. The heating and glycation-induced changes of the protein structure can affect both digestibility and immunoreactivity. The immunological consequences may be due to changes in the peptide profile of the digested dry heated milk protein. Therefore, cow’s milk protein powder was heated at low temperature (60 °C) and high temperature (130 °C) and applied to simulated infant in vitro digestion. Digestion-derived peptides after 10 min and 60 min in the intestinal phase were measured using LC-MS/MS. Moreover, digests after 10 min intestinal digestion were applied to a Caco-2 cell monolayer. T-cell epitopes were analysed using prediction software, while specific immunoglobin E (sIgE) binding epitopes were identified based on the existing literature. The largest number of sIgE binding epitopes was found in unheated samples, while T-cell epitopes were equally represented in all samples. Transport of glycated peptide indicated a preference for glucosyl lysine and lactosyl-lysine-modified peptides, while transport of peptides containing epitope structures was limited. This showed that the release of immunoreactive peptides can be affected by the applied heating conditions; however, availability of peptides containing epitopes might be limited.

Faecal calprotectin as an aid to the diagnosis of non-IgE mediated cow's milk protein allergy

2016 ◽  
Vol 84 (6) ◽  
pp. 318-323 ◽  
Author(s):  
Carlos Trillo Belizón ◽  
Eduardo Ortega Páez ◽  
Antonio Medina Claros ◽  
Isabel Rodríguez Sánchez ◽  
Ana María Reina González ◽  
...  
Keyword(s):  
Milk Protein ◽  
Cow’S Milk ◽  
Faecal Calprotectin ◽  
Cow's Milk ◽  
Ige Mediated ◽  
Cow’S Milk Protein

Screening for Cow Milk Allergy Among Young Egyptian Children

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Zeinab A El-Sayed ◽  
Rasha H El-Owaidy ◽  
Hanan M Abd El-Lateef ◽  
Ahmed SA Hammouda
Keyword(s):  
Milk Protein ◽  
Clinical History ◽  
Cow Milk ◽  
Cow’S Milk ◽  
Published Data ◽  
Cow's Milk ◽  
Oral Challenge Test ◽  
Egyptian Children ◽  
Ige Mediated ◽  
Cow’S Milk Protein

Abstract Background There are insufficient published data on the prevalence of cow's milk protein allergy (CMPA) in Egypt. Aim of the Work The study aims to estimate the frequency of CMPA among young Egyptian children in a trial to delineate the magnitude of the problem in our population. Methods A cross-sectional, observational study was conducted on 500 young Egyptian children from the general population aged from 6 months to 5 years; 200 males and 300 females. Children were subjected to history taking and clinical examination. Suspected cases with suggestive clinical history of CMPA were subjected to skin prick test (SPT) with standardized cow’s milk protein allergen extract and open oral challenge test (OCT). Results In the present study, 6.2% of screened children had positive clinical history suggestive of CMPA, 35.5% of them had positive SPT and 51.6% had positive OCT. Based on clinical history and OCT, 3.2% were documented to have CMPA, 3% had undocumented CMPA or low probability of CMPA and 93.8% had no CMPA. The highest rate of initial onset of CMPA is seen before the first year of life (96.8%) and 81.3% of children with positive OCT were formula/mixed-fed. The clinical manifestations in infants with suspected CMPA were variable, and the main presenting feature was gastrointestinal tract manifestations (42%). The IgE-mediated CMPA was more common and constituted 56.3% of the CMPA cases. Conclusion The frequency of CMPA among Egyptian children up to 5 years of age is comparable to the reported frequencies worldwide. IgE-mediated CMPA accounts for more than 2/3 of the patients with proven CMPA in this study.

Patients suffering from non-IgE-mediated cow’s milk protein intolerance cannot be diagnosed based on IgG subclass or IgA responses to milk allergens

Allergy ◽  
2011 ◽  
Vol 66 (9) ◽  
pp. 1201-1207 ◽  
Author(s):  
H. Hochwallner ◽  
U. Schulmeister ◽  
I. Swoboda ◽  
T. E. Twaroch ◽  
H. Vogelsang ◽  
...  
Keyword(s):  
Milk Protein ◽  
Cow’S Milk ◽  
Igg Subclass ◽  
Cow's Milk ◽  
Cow's Milk Protein Intolerance ◽  
Ige Mediated ◽  
Cow’S Milk Protein ◽  
Protein Intolerance
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