Correlation between phenotypic virulence traits and antibiotic resistance in Pseudomonas aeruginosa clinical isolates

Background: Drug resistance and virulence genes are two key factors for the colonization of Pseudomonas aeruginosa in settings with high antibiotic pressure, such as hospitals, and the development of hospital-acquired infections. Objectives: The objective of this study was to investigate the prevalence of drug resistance and virulence gene profiles in clinical isolates of P. aeruginosa in Ardabil, Iran. Methods: A total of 84 P. aeruginosa isolates were collected from clinical specimens of Ardabil hospitals and confirmed using laboratory standard tests. The disk diffusion method was used for antibiotic susceptibility testing and polymerase chain reaction (PCR) for the identification of P. aeruginosa virulence genes. Results: The highest and the lowest antibiotic resistance rates of P. aeruginosa strains were against ticarcillin-clavulanate (94%) and doripenem (33.3%), respectively. In addition, the frequency of multidrug-resistant (MDR) P. aeruginosa was 55.9%. The prevalence of virulence factor genes was as follows: algD 84.5%, lasB 86.9%, plcH 86.9%, plcN 86.9%, exoU 56%, exoS 51.2%, toxA 81%, nan1 13.1%, and pilB 33.3%. A significant association was observed between resistance to some antibiotics and the prevalence of virulence genes in P. aeruginosa. Conclusions: Our results revealed a high prevalence of antibiotic resistance, especially MDR, and virulence-associated genes in clinical isolates of P. aeruginosa in Ardabil hospitals. Owing to the low resistance rates against doripenem, gentamicin, and tobramycin, these antibiotics are recommended for the treatment of infections caused by highly resistant and virulent P. aeruginosa strains.

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Biofilm Production by Pseudomonas Aeruginosa Clinical Isolates and Its Relationship with Pseudomonas Quinolone Signal (PqsA) Gene and Antibiotic Resistance

The Egyptian Journal of Medical Microbiology ◽

10.12816/0046246 ◽

2017 ◽

Vol 26 (4) ◽

pp. 19-27

Author(s):

Mervat A. M. Mostafa ◽

Walaa Abdel-Latif ◽

Rania A. M. Alaam

Keyword(s):

Pseudomonas Aeruginosa ◽

Antibiotic Resistance ◽

Clinical Isolates ◽

Pseudomonas Quinolone Signal ◽

Biofilm Production

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A large-scale comparison shows that genetic changes causing antibiotic resistance in experimentally evolved Pseudomonas aeruginosa predict those in naturally evolved bacteria

10.1101/674531 ◽

2019 ◽

Author(s):

Samuel J. T. Wardell ◽

Attika Rehman ◽

Lois W. Martin ◽

Craig Winstanley ◽

Wayne M. Patrick ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Antibiotic Resistance ◽

Experimental Evolution ◽

Large Scale ◽

Genetic Basis ◽

Clinical Isolates ◽

Resistant Bacteria ◽

Antibiotic Resistant ◽

Health Crisis ◽

Wide Range

AbstractPseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of acute and chronic infections. An increasing number of isolates have acquired mutations that make them antibiotic resistant, making treatment more difficult. To identify resistance-associated mutations we experimentally evolved the antibiotic sensitive strain P. aeruginosa PAO1 to become resistant to three widely used anti-pseudomonal antibiotics, ciprofloxacin, meropenem and tobramycin. Mutants were able to tolerate up to 2048-fold higher concentrations of antibiotic than strain PAO1. Genome sequences were determined for thirteen mutants for each antibiotic. Each mutant had between 2 and 8 mutations. There were at least 8 genes mutated in more than one mutant per antibiotic, demonstrating the complexity of the genetic basis of resistance. Additionally, large deletions of up to 479kb arose in multiple meropenem resistant mutants. For all three antibiotics mutations arose in genes known to be associated with resistance, but also in genes not previously associated with resistance. To determine the clinical relevance of mutations uncovered in experimentally-evolved mutants we analysed the corresponding genes in 457 isolates of P. aeruginosa from patients with cystic fibrosis or bronchiectasis as well as 172 isolates from the general environment. Many of the genes identified through experimental evolution had changes predicted to be function-altering in clinical isolates but not in isolates from the general environment, showing that mutated genes in experimentally evolved bacteria can predict those that undergo mutation during infection. These findings expand understanding of the genetic basis of antibiotic resistance in P. aeruginosa as well as demonstrating the validity of experimental evolution in identifying clinically-relevant resistance-associated mutations.ImportanceThe rise in antibiotic resistant bacteria represents an impending global health crisis. As such, understanding the genetic mechanisms underpinning this resistance can be a crucial piece of the puzzle to combatting it. The importance of this research is that by experimentally evolving P. aeruginosa to three clinically relevant antibiotics, we have generated a catalogue of genes that can contribute to resistance in vitro. We show that many (but not all) of these genes are clinically relevant, by identifying variants in clinical isolates of P. aeruginosa. This research furthers our understanding of the genetics leading to resistance in P. aeruginosa and provides tangible evidence that these genes can play a role clinically, potentially leading to new druggable targets or inform therapies.

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Study of Antibiotic Resistance by Efflux in Clinical Isolates of Pseudomonas aeruginosa

Pakistan Journal of Biological Sciences ◽

10.3923/pjbs.2007.924.927 ◽

2007 ◽

Vol 10 (6) ◽

pp. 924-927 ◽

Cited By ~ 7

Author(s):

A. Abdi-Ali ◽

A. Rahmani-Ba ◽

T. Falsafi ◽

V. Nikname

Keyword(s):

Pseudomonas Aeruginosa ◽

Antibiotic Resistance ◽

Clinical Isolates

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Antibiotic resistance and bacteriophages commercial drugs susceptibility of Klebsiella pneumonia and Pseudomonas aeruginosa clinical strains

Sanitarnyj vrač (Sanitary Inspector) ◽

10.33920/med-08-2109-08 ◽

2021 ◽

pp. 73-80

Author(s):

D. A. Sedova ◽

A. S. Kaljuzhin ◽

Juliya Alexandrovna Romanovskaya

Keyword(s):

Pseudomonas Aeruginosa ◽

Antibiotic Resistance ◽

Klebsiella Pneumoniae ◽

High Resistance ◽

Hemolytic Activity ◽

Clinical Isolates ◽

Klebsiella Pneumonia ◽

Clinical Strains ◽

Resistant Strains ◽

High Degree

The article provides information about hemolytic activity, susceptibility to different groups of antibiotics and bacteriophages commercial drugs of Klebsiella pneumoniae and Pseudomonas aeruginosa clinical strains. The analysis of the obtainedresults showed a high degree of hemolytic activity dissemination among Pseudomonas aeruginosa (78,60 % of strains), as well as their high resistance to cefotaxime, chloramphenicol, azithromycin and tetracycline (82,14–100 % of resistant strains). K. pneumoniae clinical isolates were resistant to cefazolin, azithromycin, and the nitrofuran group (66,67–81,48 %). In turn, specific phagolysates for treatment deseases of K. pneumoniae and P. aeruginosa etiology showed the greatest efficiency against both groups of microorganisms among the studied commercial preparations of bacteriophages.

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