Observation of angiotensin II-induced changes in fixed and live mesangial cells by atomic force microscopy

Micron ◽  
2010 ◽  
Vol 41 (3) ◽  
pp. 220-226 ◽  
Author(s):  
Gi-Ja Lee ◽  
Eun-Jin Park ◽  
Samjin Choi ◽  
Jeong-Hoon Park ◽  
Kyung-Hwan Jeong ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Angiotensin Ii ◽  
Mesangial Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Induced Changes

Intercalation-induced changes in DNA supercoiling observed in real-time by atomic force microscopy

1999 ◽  
Vol 400 (1-3) ◽  
pp. 27-32 ◽  
Author(s):  
L.H Pope ◽  
M.C Davies ◽  
C.A Laughton ◽  
C.J Roberts ◽  
S.J.B Tendler ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Real Time ◽  
Dna Supercoiling ◽  
Force Microscopy ◽  
Atomic Force ◽  
Induced Changes

scholarly journals The effect of Galleria mellonella hemolymph polypeptides on Legionella gormanii.

2014 ◽  
Vol 61 (1) ◽  
Author(s):  
Elżbieta Chmiel ◽  
Marta Palusinska-Szysz ◽  
Agnieszka Zdybicka-Barabas ◽  
Małgorzata Cytryńska ◽  
Paweł Mak
Keyword(s):  
Atomic Force Microscopy ◽  
Survival Rate ◽  
Galleria Mellonella ◽  
Community Acquired Pneumonia ◽  
Force Microscopy ◽  
Apolipophorin Iii ◽  
Atomic Force ◽  
Legionella Species ◽  
Induced Changes ◽  
First Time

Among Legionella species, which are recognized to be pathogenic for humans, L. gormanii is the second prevalent causative agent of community-acquired pneumonia after L. pneumophila. Anti-L. gormanii activity of Galleria mellonella hemolymph extract and apolipophorin III (apoLp-III) was examined. The extract and apoLp-III at the concentration 0.025 mg/ml caused 75% and 10% decrease of the bacteria survival rate, respectively. The apoLp-III-induced changes of the bacteria cell surface were analyzed for the first time by atomic force microscopy. Our studies demonstrated the powerful anti-Legionella effects of the insect defence polypeptides, which could be exploited in drugs design against these pathogens.

scholarly journals Observations of the Effects of Angiotensin II Receptor Blocker on Angiotensin II-Induced Morphological and Mechanical Changes in Renal Tubular Epithelial Cells Using Atomic Force Microscopy

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Jin Sug Kim ◽  
Gi-Ja Lee ◽  
Tae Won Lee ◽  
Chun Gyoo Ihm ◽  
Yu Ho Lee ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Atomic Force Microscopy ◽  
Angiotensin Ii ◽  
Epithelial Cells ◽  
Cell Surface ◽  
Angiotensin Ii Receptor Blocker ◽  
Ang Ii ◽  
Tubular Epithelial Cells ◽  
Force Microscopy ◽  
Atomic Force

Objective. Angiotensin II (Ang II) plays a profibrotic role in the kidneys. Although many pathways of Ang II have been discovered, the morphological and mechanical aspects have not been well investigated. We observed the changes in tubular epithelial cells (TECs) after Ang II treatment with or without Ang II receptor blockers (ARBs) using atomic force microscopy (AFM). Methods. TECs were stimulated with Ang II with or without telmisartan, PD123319, and blebbistatin. AFM was performed to measure the cellular stiffness, cell volume, and cell surface roughness. Epithelial to mesenchymal transition markers were determined via immunocytochemistry. Results. After Ang II stimulation, cells transformed to a flattened and elongated mesenchymal morphology. Cell surface roughness and volume significantly increased in Ang II treated TECs. Ang II also induced an increase in phospho-myosin light chain and F-actin and a decrease in E-cadherin. Ang II coincubation with either telmisartan or blebbistatin attenuated these Ang II-induced changes. Conclusion. We report, for the first time, the use of AFM in directly observing the changes in TECs after Ang II treatment with or without ARBs. Simultaneously, we successfully measured the selective effect of PD123319 or blebbistatin. AFM could be a noninvasive evaluating strategy for cellular processes in TECs.

Sign in / Sign up

Export Citation Format

Share Document