Long-term preservation of Tetraselmis indica (Chlorodendrophyceae, Chlorophyta) for flow cytometric analysis: Influence of fixative and storage temperature

2017 ◽  
Vol 139 ◽  
pp. 123-129 ◽  
Author(s):  
Sangeeta Mahableshwar Naik ◽  
Arga Chandrashekar Anil
Keyword(s):  
Storage Temperature ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
And Storage ◽  
Long Term Preservation

scholarly journals Temperature Effects on Interspecific Hybridization between Gladiolus ×grandiflora and G. tristis

HortScience ◽  
2001 ◽  
Vol 36 (2) ◽  
pp. 341-343 ◽  
Author(s):  
Yasumasa Takatsu ◽  
Masakazu Kasumi ◽  
Toru Manabe ◽  
Mikio Hayashi ◽  
Eiichi Inoue ◽  
...  
Keyword(s):  
Interspecific Hybridization ◽  
Wild Species ◽  
Storage Temperature ◽  
Pollen Viability ◽  
Flow Cytometric Analysis ◽  
Morphological Data ◽  
Modern Cultivar ◽  
Long Term Storage ◽  
Flow Cytometric

Interspecific hybridization between a modern cultivar of Gladiolu×grandiflora hort. (2n = 60) and the wild species G. tristis L. (2n = 30) was made to introduce characteristics of the wild species into the cultivated one. Gladiolus ×grandiflora is a summer-flowering species, and G. tristis flowers in winter. The effect of storage temperature on pollen viability was tested, as long-term storage of pollen was necessary to facilitate crossing these two species. Pollen of G. tristis could be stored at -20 °C for ≈1 year, and was more practical than storage at -80 °C. Air temperature affected pollen tube growth, fertility, and fruit set in the cross between G. ×grandiflora and G. tristis, and low temperatures (15 to 20 °C) were best. The morphological data and flow cytometric analysis showed that the F1 plants were hybrids between G. ×grandiflora and G. tristis.

scholarly journals Long-term hydrophilization of polydimethylsiloxane (PDMS) for capillary filling microfluidic chips

2019 ◽  
Vol 24 (1) ◽  
Author(s):  
Farzin Jahangiri ◽  
Tuuli Hakala ◽  
Ville Jokinen
Keyword(s):  
Cold Storage ◽  
Storage Temperature ◽  
Microfluidic Channel ◽  
Vibration Band ◽  
Microfluidic Chips ◽  
Thermally Activated ◽  
Capillary Filling ◽  
Different Temperatures ◽  
Long Term Preservation

AbstractWe present a simple and facile method for long-term preservation of hydrophilicity of oxygen plasma-hydrophilized poly (dimethylsiloxane) (PDMS) by cold storage. We show that storage under temperature of − 80 °C can maintain superhydrophilicity of plasma-exposed PDMS for at least 100 days. Storage at − 15 °C and at 22 °C room temperature (RT) is shown to exhibit, respectively, about half and full recovery of the original hydrophobicity after 100 days in storage. Furthermore, we investigated the implications of the cold storage for microfluidic applications, the capillary filling rate and the ability of the flow to bypass geometrical obstacles in a microfluidic channel. It is shown that the preservation of capillary filling properties of microchannels is in close agreement with the contact angle (CA) measurements and that the colder the storage temperature, the better the capillary filling capability of the channels is preserved. We ascribe the significantly reduced recovery rate to reduced thermally activated relaxation phenomena such as diminished diffusion of low molecular weight species (LMW) in the polymer matrix at colder temperatures. This is supported by ATR-FTIR measurements of the OH vibration band over time for samples stored at different temperatures.

scholarly journals Impact of collection, isolation and storage methodology of circulating microvesicles on flow cytometric analysis

2015 ◽  
Vol 10 (6) ◽  
pp. 2093-2101 ◽  
Author(s):  
FANCONG KONG ◽  
LIMING ZHANG ◽  
HONGXIANG WANG ◽  
GUOLIN YUAN ◽  
ANYUAN GUO ◽  
...  
Keyword(s):  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
And Storage

scholarly journals 069 Preservation of Lettuce Seed for 30 Years: Cryopreservation Retarded Deterioration

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 400D-400
Author(s):  
P.C. Stanwood ◽  
L. Wheeler ◽  
L.E. Towill
Keyword(s):  
Storage Temperature ◽  
Seed Vigor ◽  
Lettuce Seed ◽  
Seed Moisture Content ◽  
Fresh Weight Basis ◽  
C Storage ◽  
Seed Moisture ◽  
Nitrogen Vapor ◽  
Long Term Preservation

Long-term preservation of seed germplasm is a high agricultural priority. It assures that genetic diversity will be available for future generations for continued plant improvement. This experiment reports on the affect that storage temperature had on the viability of 65 selections of lettuce seed stored for 30 years. The average seed moisture content was 5.5% ± 0.5% (fresh weight basis). Fresh seed samples were placed at 5 °C storage in 1969. In 1975 they were then transferred to -18 °C storage. Viability remained at 98% ± 5% for the first 14 years of 5 /-18 °C storage, then viability declined. At 17 years storage, the average viability had dropped to 75% and continued to drop at about 4%/year. At the 17-year mark, individual samples were split, one-half remained at -18 °C the other half was placed under liquid nitrogen vapor (lnv) conditions (about -150 to -190 °C). The -18 °C stored samples continued to deteriorate to 14% viability at the 30 year test period (1999). The samples placed in lnv did not decrease further in viability and remained at 75% viability at the 30-year mark. Seed vigor was reduced in the -18 °C stored seeds that were still viable. The lnv-preserved samples were significantly more vigorous. It is clear from this experiment that lnv preservation was significantly superior to -18 °C storage and, in fact, stopped or significantly reduced the rate of viability loss in samples that are rapidly deteriorating.

scholarly journals Paroxysmal Nocturnal Hemoglobinuria: An Underestimated Cause of Pediatric Thromboembolism

TH Open ◽  
2020 ◽  
Vol 04 (01) ◽  
pp. e36-e39
Author(s):  
Christina Griesser ◽  
Michael Myskiw ◽  
Werner Streif
Keyword(s):  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Clinical Symptoms ◽  
Bone Marrow Failure ◽  
Flow Cytometric Analysis ◽  
Routine Diagnostics ◽  
Glycosyl Phosphatidylinositol ◽  
Vein Thrombosis ◽  
Flow Cytometric ◽  
Appropriate Treatment

AbstractParoxysmal nocturnal hemoglobinuria (PNH) is a chronic disease caused by complement-mediated hemolysis. Clinical symptoms include intravascular hemolysis, nocturnal hemoglobinuria, thromboses, cytopenia, fatigue, abdominal pain, and a strong tendency toward bone marrow failure. It is a rare disease, especially in children, with high mortality rates without appropriate treatment.We here present the case of a 17-year-old girl with unprovoked muscle vein thrombosis. Flow cytometric analysis showed deficiency of glycosyl-phosphatidylinositol-anchored membrane proteins on all three hematopoietic cell lines and confirmed the diagnosis of PNH. Treatment with the monoclonal antibody eculizumab achieved long-term remission.As flow cytometry is normally not part of the routine diagnostics for pediatric thrombosis, awareness is crucial and PNH is important to consider in all children with thrombosis at atypical sites and abnormalities in blood counts with regard to hemolysis and cytopenia.

Effects of Sucrose Ester, Dough Conditioner, and Storage Temperature on Long-Term Textural Stability of Shelf-Stable Bread

2002 ◽  
Vol 79 (6) ◽  
pp. 806-811 ◽  
Author(s):  
Ann Barrett ◽  
Armand Cardello ◽  
Paul Maguire ◽  
Michelle Richardson ◽  
Gonul Kaletunc ◽  
...  
Keyword(s):  
Storage Temperature ◽  
Sucrose Ester ◽  
Shelf Stable ◽  
And Storage

scholarly journals Preservation of Azotobacter chroococcum vegetative cells in dry polymers

2014 ◽  
Vol 20 (2) ◽  
pp. 201 ◽  
Author(s):  
Daniel Rojas-Tapias ◽  
Oriana Ortega Sierra ◽  
Diego Rivera Botía ◽  
Ruth Bonilla
Keyword(s):  
Storage Conditions ◽  
Azotobacter Chroococcum ◽  
Bacterial Degradation ◽  
Protective Agent ◽  
Degradation Rates ◽  
Different Temperatures ◽  
And Storage ◽  
Temperature Time ◽  
Long Term Preservation

We studied the preservation of Azotobacter chroococcum C26 using three dry polymers: carrageenin, sodium alginate, and HPMC, using a method of accelerated degradation. Bacterial viability, as response variable, was measured at three temperatures in four different times, which was followed by calculation of bacterial degradation rates. Results showed that temperature, time of storage, and protective agent influenced both viability and degradation rates (P;lt;0.05). We observed, using the Arrhenius thermodynamic model, that the use of polymers increased the activation energy of bacterial degradation compared to control. We obtained thermodynamic models for each polymer, based on the Arrhenius equation, which predicted the required time for thermal degradation of the cells at different temperatures. Analysis of the models showed that carrageenin was the best polymer to preserve A. chroococcum C26 since ~ 900 days are required at 4 ºC to reduce its viability in two log units. We conclude, therefore, that long-term preservation of A. chroococcum C26 using dry polymers is suitable under adequate preservation and storage conditions.

Immunohistochemical and Flow Cytometric Analysis of Long-Term Label-Retaining Cells in the Adult Heart

2011 ◽  
Vol 20 (2) ◽  
pp. 211-222 ◽  
Author(s):  
Andrea Meinhardt ◽  
Albert Spicher ◽  
Marc-Estienne Roehrich ◽  
Ingmar Glauche ◽  
Pierre Vogt ◽  
...  
Keyword(s):  
Flow Cytometric Analysis ◽  
Adult Heart ◽  
Flow Cytometric
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