The Imbibition, Viability, and Germination of Caper Seeds (Capparisspinosa L.) in the First Year of Storage

The caper is a shrub that adapts to harsh environments when it is established, but it presents serious difficulties in its propagation, both by cuttings and by seeds. Its seeds have low germination percentages, and germination is a very slow process. Significant increases in germination have been obtained with scarification and with the addition of gibberellic acid (GA3) to the substrate, leading to the hypothesis that they have possible physical and physiological dormancy. However, the only way to examine the water-impermeability of the cover is through imbibition analysis. This study analyzes the imbibition, viability, and germination of two seed lots, obtained in different years and evaluated immediately after their collection (FS) and after being stored (7 °C) for one month (DS) and one year (SS). The seed moisture content stabilizes from the fourth day, exceeding in all cases 31% in all three seed states tested (FS, DS and SS). This allows the germination of all viable seeds, only with the addition of GA3 to the germination substrate, without the need for scarification, so that caper seeds exclusively appear to present a physiological latency. Germination decreased in storage, even with just one month. With the GA3 addition, high germination values were obtained (up to 95% in FS).

Measurements and Analysis of the Physical Properties of Cereal Seeds Depending on Their Moisture Content to Improve the Accuracy of DEM Simulation

This article presents the results of research on the influence of moisture on changes in the physical properties, i.e., the length, width, thickness, and weight, of dressed and untreated cereal seeds in order to improve the simulation process based on the discrete element method (DEM). The research was conducted on the seeds of three winter cereals, i.e., triticale, rye, and barley. The seeds with an initial moisture content of about 7% were moistened to five levels, ranging from 9.5% to 17.5%, at an increment of 2%. The statistical analysis showed that moisture significantly influenced the physical properties of the seeds, i.e., their length, width, thickness, and weight. As the moisture content of the seeds increased, there were greater differences in their weight. The average increase in the thousand kernel weight resulting from the increase in their moisture content ranged from 4 to 6 mg. The change in the seed moisture content from 9.5% to 17.5% significantly increased the volume of rye seeds from 3.10% to 14.99%, the volume of triticale seeds from 1.00% to 13.40%, and the volume of barley seeds from 1.00% to 15.33%. These data can be used as a parameter to improve the DEM simulation process.

Potensi Produksi dan Mutu Benih serta Produksi Biomassa Sorghum bicolor Varietas Samurai 2 pada Umur Panen Berbeda sebagai Bahan Pakan

Sorghum bicolor var. Samurai 2 can be used as raw material in silage production for ruminant feed. The problem encountered is the difficulty of obtaining certified seeds for commercial sorghum production. So that is necessary to do this research to investigate potential sorghum seed production and its quality of Sorghum bicolor var. Samurai 2. The study was conducted at University Research Station-Jonggol Animal Education and Research Unit, Bogor Agricultural University. The experimental design used was a randomized block design with 4 treatments and 5 replicates. Five individual plants were taken to measure the variables at each treatment set. The treatments consisted of different harvesting times, namely P95 (harvested 95 days after planting), P100, P105 and P110. The variables observed were dry weight of shelled seeds, seed weight per panicle, weight of panicle stalk, panicle weight, seed production per ha, seed moisture content, seed viability test, and shoot biomass production per ha. The results showed that seed production per ha, panicle dry matter weight, fresh seed moisture content, panicle stalk dry weight was not significantly different. Dry weight of shelled seeds, dry weight of seeds per panicle, panicle dry weight, seed viability, weight of biomass per ha were significantly different (p<0.05). The potential for the production of shelled seeds, dry matter of seeds per panicle was the best in the P105 and P110, the viability of the seeds in the P105 and shoot biomass production per ha in the P105. The potential for shelled seed production (4038 kg ha-1), seed dry weight per panicle (54.87 g panicle-1), seed viability (92.8%) and the best biomass production (55.88 tons ha-1) were in treatment P105. Key words: seed production, shoot biomass, Sorghum bicolor, viability

Interrelationship Among Seed Quality Parameters and Fungi Associated with Seeds of Cotton (Gossypium Hirsutum L.)

A total of twenty nine species of fungi namely Aspergillus aculeatus Lizuka, A. flavus Link, A. fumigatus Fresenius, A. niger Van Tiegh, A. nidulans Eidam, A. subramanianii Visagie, Frisvad & Samson, A. tamariiKita G., A. toxicarius Murak, A. wentii Wehmer, Curvularial unata (Wakker) Boedijn, Colletotrichum gloeosporioides (Penz.) Sacc., C. gossypii Southw., Chaetomium globosum Kunze., Fusarium moniliforme J. Shelden, F. nivale (Fr.) Sorauer, F. oxysporum Schlechtendal, F. fujikuroi Nirenberg, F. solani(Mart.) Sacc., Lasiodiplodiatheobromae (Pat.) Griffon & Maubl., Meyerozyma guilliermondii (Wick.) Kurtzman & M. Suzuki., Mucor sp. P. Micheli ex L., Penicillium aculeatum Raper& Fennell, Penicillium citrinum Thom, Rhizoctonia solani Khun., Rhizopus stolonifer (Ehrenb.) Vuill., Rhizopus oryzae Went & Prins. Geerl., Rhizo mucor sp. Luce t & Costantin, Syncephalastrum racemosum Cohn and Trichoderma viride Pers. were found to be associated with the seeds of 14 varieties viz. CB-1 to CB-14 of cotton. Seed quality analysis showed that percentage of pure seeds, germination, moisture, seedling mortality and associated fungi with different varieties of cotton seeds varies from 97.08-99.92%, 80-93%, 10-11.3%, 16.05-50.30% and 24-78.0% respectively. Present study deals with estimation of interrelationship between some quality factors through correlation and regression analysis are important for the assessment of seed quality. There were negative correlations between seedling mortality and purity percentage and between germination rate and fungus frequency. On the other hand, positive correlations were found between germination rate and purity percentage, between seedling mortality and fungus frequency, between purity percentage and fungus frequency, between germination rate and seed moisture and between fungus frequency and seed moisture. CB 8 was superior from the other cotton variety as it showed higher physical purity of seed (98.41%), higher seed germination (92%), lower fungal incidence (24%) and lower mortality of seedling (23.91%). Bioresearch Commu. 8(1): 1061-1067, 2022 (January)

COMPARISON OF TWO MICROWAVE DRYING TECHNIQUES FOR CEREAL SEEDS AND DETERMINATION OF THEIR PHYSICAL PARAMETERS

The microwave drying of wheat and corn seeds by two technologies at different working powers is studied here. The main objective is to evaluate the effect of microwaves on variations in the moisture, colour and size of the seeds after microwave drying. The tests are performed in microwave ovens with and without inverter technology at three drying powers (260, 440 and 620 W). It has been observed that increasing drying power results in a ~50% reduction in drying time and a seed moisture level below 14%. The colour variation of the seeds is insignificant between microwave drying with and without the inverter. However, there is also an unacceptable decrease in size for both types of seeds with increasing drying power, which can lead to increased shrinkage of the seeds and the appearance of cracks, especially in the corn seeds.

PENGARUH FLY ASH DAN LEGIN TERHADAP PERKEMBANGAN BIJI DAN PRODUKSI KACANG HIJAU (Vigna radiata L)

The purpose of the research was to know the effect of Fly Ash and legin interaction on seed development and green bean production (Vigna radiata L), and the main influence of each treatment. The study used Completely Randomized Design (RAL) that consisted of two factors: Fly Ash waste with 4 treatment levels, i.e without fly ash, giving flay ash 1.0; 1.5 and 2.0 kg plots, and Legin Inoculation with 4 treatment levels, i.e without legin, inoculation of legin 5.0; 10.0 and 15.0 g / kg of seed. The results showed that the interaction of legin and fly ash inoculation did not affect the growth of green bean seeds, such as changes in seed moisture content, changes in dry seed weight, the rate of dry matter seed collecting (KPBK), effective seeding time (WPE), number of pods and weight of 100 grains seed. However, it affected the percentage of pods and the weight of dry seed harvest. Inoculation of 10 g of legin and 1.5 kg of fly ash was sufficient to produce the highest yield. Legin inoculation affected all parametrs observed. Inokulasi green beans with 10.0 g legin/kg seeds show the best seed development and production for all parameters that were observed. Fly ash feed affected all observation parameters. Giving 1.5 kg of fly ash/plot was sufficient for green bean plants to produce high seed development and production.

Evaluation of a seed storage facility in relation to preserving seed moisture, vigour and germination

The performance of a solar energy-assisted seed storage room was evaluated through an ordinary 22-m3 room that was retrofitted with a solar collector, inlets and chimney. The structure was made of a solar collector to heat the ambient air before entering the chimney. The chimney circulated the air inside the structure and inlets. To compare the performance of the modified storage room, a room with a similar capacity and without the retrofitted components (control storage room) was used. Twelve 8 kg bags of maize were stored in each storage room for a period of three months. Samples were taken every two weeks to determine germination rate, moisture content and seed vigour. The temperature and relative humidity (RH) was measured during storage. The RH in the control storage was significantly higher (P≤0.05) (60.6 ± 5.87%) than in the modified storage (40.1 ± 3.21%) during the day. However, at night, the RH in the control storage room was significantly lower (P≤0.05) (58.5 ± 7.32%) than in the modified storage (63.7 ± 6.28%). The RH in the modified storage room increased from 40.1% during the day to 63.7% at night. The RH in the control storage room decreased slightly from 60.6% to 58.5% during the day and night. The seed moisture content in the modified storage facility was significantly lower (P≤0.05) (12.6 ± 0.21%) than in the control storage room (13.3 ± 0.52%). The moisture content in the modified storage room decreased from 12.6% to 12.4%, whereas in the control room, moisture content increased from 12.6% to 13.8% in three months. The seed germination rate obtained after three months of storage in the modified storage room was significantly higher (P≤0.05) (98.5 ± 0.85%) than in the control storage room (96.8 ± 1.49%).The seed vigour obtained in the modified storage room was significantly higher (93.6 ± 0.35%) than in the control room (91.7 ± 2.08%) (P≤0.05). Seed stored in the control storage lost vigour at a faster rate, compared to the seeds stored in the modified storage room. Therefore, the modified naturally-ventilated seed storage room maintained seed quality better than the control storage room.