scholarly journals 069 Preservation of Lettuce Seed for 30 Years: Cryopreservation Retarded Deterioration

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 400D-400
Author(s):  
P.C. Stanwood ◽  
L. Wheeler ◽  
L.E. Towill
Keyword(s):  
Storage Temperature ◽  
Seed Vigor ◽  
Lettuce Seed ◽  
Seed Moisture Content ◽  
Fresh Weight Basis ◽  
C Storage ◽  
Seed Moisture ◽  
Nitrogen Vapor ◽  
Long Term Preservation

Long-term preservation of seed germplasm is a high agricultural priority. It assures that genetic diversity will be available for future generations for continued plant improvement. This experiment reports on the affect that storage temperature had on the viability of 65 selections of lettuce seed stored for 30 years. The average seed moisture content was 5.5% ± 0.5% (fresh weight basis). Fresh seed samples were placed at 5 °C storage in 1969. In 1975 they were then transferred to -18 °C storage. Viability remained at 98% ± 5% for the first 14 years of 5 /-18 °C storage, then viability declined. At 17 years storage, the average viability had dropped to 75% and continued to drop at about 4%/year. At the 17-year mark, individual samples were split, one-half remained at -18 °C the other half was placed under liquid nitrogen vapor (lnv) conditions (about -150 to -190 °C). The -18 °C stored samples continued to deteriorate to 14% viability at the 30 year test period (1999). The samples placed in lnv did not decrease further in viability and remained at 75% viability at the 30-year mark. Seed vigor was reduced in the -18 °C stored seeds that were still viable. The lnv-preserved samples were significantly more vigorous. It is clear from this experiment that lnv preservation was significantly superior to -18 °C storage and, in fact, stopped or significantly reduced the rate of viability loss in samples that are rapidly deteriorating.

scholarly journals Storage of seeds of Calamus palustris var. cochinchinensis

2020 ◽  
Vol 48 (2) ◽  
pp. 201-207
Author(s):  
Y.K. Fan ◽  
M. Liu ◽  
J.X. Hu ◽  
M.Y. Ji ◽  
Q.Y. Lan
Keyword(s):  
Moisture Content ◽  
Water Content ◽  
Storage Temperature ◽  
Aluminum Foil ◽  
Effect Of Temperature ◽  
Seed Moisture Content ◽  
Seed Desiccation ◽  
Seed Moisture ◽  
Mature Seeds

The present study examined the effect of temperature (15, 20, 25, 30 and 20/30°C) on germination and the storage behaviour of freshly harvested mature seeds of Calamus palustris var. cochinchinensis. Seed desiccation tolerance and the effects of storage temperature (4 and 15°C), perlite water content (120, 180 and 240%) and seed moisture content (27.8, 38.2 and 49.2%) on viability were observed. Seeds had a higher germination at 25°C (88.3%) than at the other tested temperatures. Germination decreased as the seed moisture content decreased during desiccation. The germination of seeds stored at 15°C was higher than that of seeds stored at 4°C. Germination of seeds stored at 15 and 4°C was <65% and with extension of storage time, the germination decreased, indicating that neither temperature can be used for long-term conservation. For short-term storage, the seeds can be stored at 15°C with perlite with 180% water content in plastic bottles or at 15°C with 49.2% moisture content sealed inside aluminum foil bags.

scholarly journals Effect of Scarification, Seed Storage Temperature, and Relative Humidity on Lupinus havardii Wats. and Lupinus texensis Hook. Seed Germination

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 782-785 ◽  
Author(s):  
Wayne A. Mackay
Keyword(s):  
Relative Humidity ◽  
Storage Temperature ◽  
Germination Rate ◽  
Seed Storage ◽  
Significant Decline ◽  
Rapid Decline ◽  
Seed Moisture Content ◽  
C Storage ◽  
Seed Moisture ◽  
Seed Scarification

Seeds of Lupinus havardii Wats. and L. texensis Hook. were subjected to scarification, storage temperature (4 or 22 °C), and relative humidity (RH) treatments (11%, 23%, 52%, 75%, or 97% RH) for 12 months. Seed moisture increased as relative humidity increased with scarified seed having the greatest increase in seed moisture content regardless of storage temperature. For both species, the combination of seed scarification before storage, 75% RH, and 22 °C storage temperature resulted in a significant and rapid decline in germinability beginning at 4 months. Scarified L. texensis seed stored at 52% RH and 22 °C also exhibited a significant decline in germinability following 6 months storage. Seed of both species stored under all other conditions germinated similar to or higher than the initial germination rate after 12 months. These results clearly show that scarification can be performed before seed packaging as long as the seed packets are stored at ≤23% RH under 4 or 22 °C with no loss in germinability for at least 1 year.

Seed Dormancy in Red Rice (Oryza sativa) I. Effect of Temperature on Dry-Afterripening

Weed Science ◽  
1981 ◽  
Vol 29 (4) ◽  
pp. 402-404 ◽  
Author(s):  
Marc A. Cohn ◽  
J. A. Hughes
Keyword(s):  
Oryza Sativa ◽  
Seed Dormancy ◽  
Exposure Time ◽  
Storage Temperature ◽  
Effect Of Temperature ◽  
Red Rice ◽  
Seed Moisture Content ◽  
Dry Storage ◽  
C Storage ◽  
Seed Moisture

The effect of postharvest storage temperature (–15, 5, 20, and 30 C) on seed dormancy of red rice [Oryza sativa L. ‘strawhulled (SH)’], an annual species, was studied. Intact and dehulled (by hand) seeds were dormant at harvest. Intact seeds were nondormant (greater than 90% germination at 30 C) after dry storage at 20 or 30 C for 4 weeks after harvest. In intact seeds stored dry at 5 C, the degree of dormancy was dependent upon postharvest exposure time at 20 C prior to storage. Dormancy of intact seeds decreased at 5 C as this initial postharvest exposure to 20 C was increased from 2 to 7 days. This effect of initial 20 C exposure was independent of seed moisture content (11 to 12%). After dry storage of intact seeds at 5 C, dehulling promoted germination. Germination of such dehulled seeds increased with increasing storage time at 5 C up to 11 months when complete germination occurred. The response of seeds dehulled immediately after dry storage at 5 C was independent of prior exposure time (2 to 7 days) at 20 C. Storage at −15 C prevented all forms of dry-afterripening for 1 yr.

scholarly journals Accelerated aging and controlled deterioration for the determination of the physiological potential of onion seeds

2003 ◽  
Vol 60 (3) ◽  
pp. 465-469 ◽  
Author(s):  
Angelica Brod Rodo ◽  
Julio Marcos Filho
Keyword(s):  
Moisture Content ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Seed Moisture Content ◽  
Control Programs ◽  
Traditional Procedure ◽  
Accelerated Aging Test ◽  
Seed Moisture ◽  
Controlled Deterioration

International research on vegetable seed vigor is not at the same level attained for grain crops species. This study was conducted to identify reliable procedures for the accelerated aging and controlled deterioration tests to rank onion (Allium cepa L.) seed lots according to their physiological potential. Six seed lots of the cultivars Aurora and Petroline were evaluated in the laboratory for germination, first count, seedling vigor classification, traditional and saturated salt accelerated aging (41ºC / 48 and 72 h), controlled deterioration (24% of water / 45ºC / 24 h) and seedling emergence tests. Seed moisture content after the saturated salt accelerated aging test was lower and uniform, which is considered an important advantage in comparison to the traditional procedure. The saturated salt accelerated aging (41ºC / 48 and 72 h) and controlled deterioration (moisture content adjusted to 24% / 45ºC / 24 h) tests were the best procedures to assess the physiological potential of onion seeds, and are indicated for use in quality control programs.

scholarly journals Long-term hydrophilization of polydimethylsiloxane (PDMS) for capillary filling microfluidic chips

2019 ◽  
Vol 24 (1) ◽  
Author(s):  
Farzin Jahangiri ◽  
Tuuli Hakala ◽  
Ville Jokinen
Keyword(s):  
Cold Storage ◽  
Storage Temperature ◽  
Microfluidic Channel ◽  
Vibration Band ◽  
Microfluidic Chips ◽  
Thermally Activated ◽  
Capillary Filling ◽  
Different Temperatures ◽  
Long Term Preservation

AbstractWe present a simple and facile method for long-term preservation of hydrophilicity of oxygen plasma-hydrophilized poly (dimethylsiloxane) (PDMS) by cold storage. We show that storage under temperature of − 80 °C can maintain superhydrophilicity of plasma-exposed PDMS for at least 100 days. Storage at − 15 °C and at 22 °C room temperature (RT) is shown to exhibit, respectively, about half and full recovery of the original hydrophobicity after 100 days in storage. Furthermore, we investigated the implications of the cold storage for microfluidic applications, the capillary filling rate and the ability of the flow to bypass geometrical obstacles in a microfluidic channel. It is shown that the preservation of capillary filling properties of microchannels is in close agreement with the contact angle (CA) measurements and that the colder the storage temperature, the better the capillary filling capability of the channels is preserved. We ascribe the significantly reduced recovery rate to reduced thermally activated relaxation phenomena such as diminished diffusion of low molecular weight species (LMW) in the polymer matrix at colder temperatures. This is supported by ATR-FTIR measurements of the OH vibration band over time for samples stored at different temperatures.

Critical review of threatened species collections in the New South Wales Seedbank: implications for ex situ conservation of biodiversity

2004 ◽  
Vol 10 (4) ◽  
pp. 221 ◽  
Author(s):  
C. A. Offord ◽  
M. L. McKensy ◽  
P. V. Cuneo
Keyword(s):  
Moisture Content ◽  
Threatened Species ◽  
New South ◽  
Ex Situ ◽  
Seed Moisture Content ◽  
Long Term Storage ◽  
South Wales ◽  
Seed Moisture ◽  
Term Storage

This article reviews the germinability and viability of seeds of threatened species collections in the New South Wales (NSW) Seedbank with the manifold aims of: ensuring that existing storage treatments and conditions provide effective ex situ storage of threatened species seed; providing baseline viability and seed storage life data on threatened species; and, identifying research gaps in seed germination and storage protocols for threatened species and communities. The germinability and viability of a range of seed accessions, of various ages and stored under different (although mainly identifiable) conditions in the NSW Seedbank, was determined through germination and cut-tests. The results indicated that many of the Fabaceae, Myrtaceae and Proteaceae species tested are orthodox and can be stored at 5 to 10% moisture content at 5�C for up to ten years without significant loss of viability (short- to medium-term storage). The best results were obtained in the lower seed moisture content range (2 to 9%), which appeared to be especially critical for long-term storage of many Proteaceae accessions, reinforcing the need to attain the correct seed moisture content for long-term storage. Around 10% of accessions exhibited some degree of dormancy even after long storage periods. Storage of the widest range of species, for periods greater than ten years for long-term conservation purposes, is generally best conducted by storing at sub-zero temperatures. Freezing at -18�C had little effect on the germinability of a range of seeds tested and is recommended over storage at 5�C. Collection and seed banking procedures for the NSW Seedbank will be regularly reviewed and procedures modified in order to identify the best long-term storage conditions for species within this and other seedbanks. Seed collection strategies to maximize diversity and uses of seedbanks in conservation are discussed.

scholarly journals Long-Term Storage of Vegetable Juices Treated by High Hydrostatic Pressure: Assurance of the Microbial Safety

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Justyna Nasiłowska ◽  
Barbara Sokołowska ◽  
Monika Fonberg-Broczek
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Storage Temperature ◽  
Plate Count ◽  
Bacterial Cells ◽  
Carrot Juice ◽  
Beetroot Juice ◽  
C Storage ◽  
Long Term Storage

Food business operators search for new, mild technologies, which extend the shelf life of product without changing the sensory and nutritional properties. High hydrostatic pressure (HHP) meets these requirements; however it also triggers sublethal injury of bacterial cells. Sublethal injuries could spoil the product during storage and potentially pose major public health concerns. This study aims to examine the changes of sublethally injured pathogens cells in two vegetable juices: carrot juice (pH 6.0-6.7) and beetroot juice (pH 4.0-4.2) that are induced by HHP (300-500 MPa). The possibilities of recovery of bacterial cells during 28 days of juices storage at two different temperatures (5°C and 25°C) were determined using plate count methods. During the entire period of storage of carrot juice at refrigerated temperature, the propagation and regeneration ofL. innocuastrains were observed. Storage at 25°C showed that the number of these bacteria drastically decreased between 14 and 21 days. The above phenomenon was not detected inE. colicase. There was no cells recovery during long-term refrigerated storage for all strains in beetroot juice. However, in some cases spoiling of this product intermittently occurred at 25°C storage temperature. This work demonstrates that carrot juice supports growth and regeneration of HHP-sublethally injuredL. innocua, while beetroot juice can be classified as a safe product.

scholarly journals Controlled deterioration test for evaluation of sunn hemp seed vigor

2015 ◽  
Vol 37 (4) ◽  
pp. 249-253 ◽  
Author(s):  
Arthur Batoqui Costa e Silva ◽  
Ícaro Monteiro Galvão ◽  
Rafael Marani Barbosa ◽  
Clíssia Barboza da Silva ◽  
Roberval Daiton Vieira
Keyword(s):  
Moisture Content ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Germination Test ◽  
Seed Moisture Content ◽  
Hemp Seed ◽  
Seed Moisture ◽  
Sunn Hemp ◽  
Controlled Deterioration ◽  
Initial Seed

The controlled deterioration test is efficient for seed vigor evaluation of several species. However, the procedures conditions are incipient, especially in respect to tropical forages such as sunn hemp, which still does not have a standardized procedure. The objective of this research was to adjust the procedures for a controlled deterioration test in Crotalaria juncea L. seeds. For this, five commercial seed lots were evaluated for water content and initial physiological potential (germination test, first germination count, germination speed index, saturated salt accelerated aging with NaCl, electrical conductivity and seedling field emergence). For controlled deterioration test, the initial seed moisture content was adjusted to 18, 21 and 24% and, subsequently, the samples were exposed at 45 °C during 24 hours. Seed germination test was conducted with the use of sand as substrate. Evaluations were performed on the fourth, fifth and sixth day after sowing. The controlled deterioration test for sunn hemp seeds should be performed with 24% seed moisture content, at 45 °C during 24 hours, and the test evaluation on the fourth day after sowing.

scholarly journals Uji Deteriorasi Terkontrol (UDT) ‎untuk Memperkirakan Masa Simpan Benih Kedelai (Glycine max (L.) Merr.)

2019 ◽  
Vol 39 (2) ◽  
pp. 136
Author(s):  
Pepi Nur Susilawati ◽  
Anis Andrini ◽  
Evi Dwi Sulistya Nugroho ◽  
Ulima ‎ Darmania Amanda
Keyword(s):  
Moisture Content ◽  
Heat Exposure ◽  
Soybean Seed ◽  
Storage Period ◽  
Seed Vigor ◽  
Limiting Factor ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Vigor Index ◽  
Split Plot

Deterioration of seeds during storage may reduce the supply of high-quality seeds which become the limiting ‎factor of soybean production in tropical countries. Controlled Deterioration Test (CDT) is one of the ‎fastest vigor testing methods. The objective of this study was to achieve the suitable moisture content and ‎duration of CDT treatment in soybean seed, then establish a model that associated with the seed vigor in ‎the field after a certain time of storage. Split-plot with 2 factors, namely seed varieties and the combination of ‎moisture content and heat exposure duration were used to determine the influence of CDT treatment. ‎Another split-plot for 2 factors, namely storage period and seed varieties were used to determine the effect ‎of shelf life. Viability parameters were observed by germination strength (GS) and seedling vigor index (VI), ‎using a rolled paper towel method. The results showed that there was an interaction between soybean seed ‎varieties and the combination of seed moisture content treatment with duration of CDT exposure. Sindoro ‎and Tanggamus varieties had higher viability and storability than other varieties. Combinations of 28% seed ‎moisture content for 24 hours CDT duration were sensitive enough to evaluate the physiological potential of ‎soybean seeds, providing information that was closely related to seed germination after stored for 1 month. ‎

Sign in / Sign up

Export Citation Format

Share Document