In vitro hyperthermia with improved colloidal stability and enhanced SAR of magnetic core/shell nanostructures

Biopolymer coated magnetite nanoparticles (MNPs) are suitable to fabricate biocompatible magnetic fluid (MF). Their comprehensive characterization, however, is a necessary step to assess whether bioapplications are feasible before expensive in vitro and in vivo tests. The MNPs were prepared by co-precipitation, and after careful purification, they were coated by chondroitin-sulfate-A (CSA). CSA exhibits high affinity adsorption to MNPs (H-type isotherm). We could only make stable MF of CSA coated MNPs (CSA@MNPs) under accurate conditions. The CSA@MNP was characterized by TEM (size ~10 nm) and VSM (saturation magnetization ~57 emu/g). Inner-sphere metal–carboxylate complex formation between CSA and MNP was proved by FTIR-ATR and XPS. Electrophoresis and DLS measurements show that the CSA@MNPs at CSA-loading > 0.2 mmol/g were stable at pH > 4. The salt tolerance of the product improved up to ~0.5 M NaCl at pH~6.3. Under favorable redox conditions, no iron leaching from the magnetic core was detected by ICP measurements. Thus, the characterization predicts both chemical and colloidal stability of CSA@MNPs in biological milieu regarding its pH and salt concentration. MTT assays showed no significant impact of CSA@MNP on the proliferation of A431 cells. According to these facts, the CSA@MNPs have a great potential in biocompatible MF preparation for medical applications.

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Efficacy of Amphiphilic Core-Shell Nanostructures Encapsulating Gentamicin in an In Vitro Salmonella and Listeria Intracellular Infection Model

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01522-09 ◽

2010 ◽

Vol 54 (8) ◽

pp. 3524-3526 ◽

Cited By ~ 7

Author(s):

A. Ranjan ◽

N. Pothayee ◽

T. P. Vadala ◽

M. N. Seleem ◽

E. Restis ◽

...

Keyword(s):

Flow Cytometry ◽

Confocal Microscopy ◽

Fluid Phase ◽

Infection Model ◽

Core Shell ◽

Intracellular Pathogens ◽

Shell Nanostructures ◽

Fluid Phase Endocytosis ◽

Effective Transport

ABSTRACT Core-shell nanostructures with nonionic amphiphilic shells and ionic cores encapsulating gentamicin were designed for therapy against intracellular pathogens, including Salmonella and Listeria. Flow cytometry and confocal microscopy showed that their uptake into J774A.1 macrophages proceeded mainly by fluid-phase endocytosis and clathrin-mediated pathways. The nanostructures were nontoxic in vitro at doses of 50 to 250 μg/ml, and they significantly reduced the amounts of intracellular Salmonella (0.53 log) and Listeria (3.16 log), thereby suggesting effective transport into the cells.

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Focused Electron-Beam Induced Deposition, In Situ TEM And Off-Axis Electron Holography Investigation of Bi-Magnetic Core-Shell Nanostructures

Microscopy and Microanalysis ◽

10.1017/s1431927619001016 ◽

2019 ◽

Vol 25 (S2) ◽

pp. 56-57

Author(s):

Trevor P. Almeida ◽

Damien McGrouther ◽

András Kovács ◽

Rafal E. Dunin-Borkowski ◽

Stephen McVitie

Keyword(s):

Electron Beam ◽

Magnetic Core ◽

Core Shell ◽

Electron Holography ◽

In Situ Tem ◽

Shell Nanostructures ◽

Electron Beam Induced Deposition

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Magnetic core/shell nanoparticle thin films deposited by MAPLE: Investigation by chemical, morphological and in vitro biological assays

Applied Surface Science ◽

10.1016/j.apsusc.2012.02.055 ◽

2012 ◽

Vol 258 (23) ◽

pp. 9250-9255 ◽

Cited By ~ 16

Author(s):

R. Cristescu ◽

C. Popescu ◽

G. Socol ◽

I. Iordache ◽

I.N. Mihailescu ◽

...

Keyword(s):

Thin Films ◽

Magnetic Core ◽

Core Shell ◽

Biological Assays

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Influence of Shell Thickness on the Colloidal Stability of Magnetic Core-Shell Particle Suspensions

Frontiers in Chemistry ◽

10.3389/fchem.2018.00201 ◽

2018 ◽

Vol 6 ◽

Cited By ~ 1

Author(s):

Frances Neville ◽

Roberto Moreno-Atanasio

Keyword(s):

Shell Thickness ◽

Colloidal Stability ◽

Magnetic Core ◽

Core Shell ◽

Particle Suspensions ◽

Shell Particle

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Trastuzumab-decorated nanoparticles for in vitro and in vivo tumor-targeting hyperthermia of HER2+ breast cancer

Journal of Materials Chemistry B ◽

10.1039/c7tb01305a ◽

2017 ◽

Vol 5 (35) ◽

pp. 7369-7383 ◽

Cited By ~ 14

Author(s):

Javad Hamzehalipour Almaki ◽

Rozita Nasiri ◽

Ani Idris ◽

Mahtab Nasiri ◽

Fadzilah Adibah Abdul Majid ◽

...

Keyword(s):

Breast Cancer ◽

Tumor Targeting ◽

Magnetic Core ◽

Core Shell ◽

Hyperthermia Treatment ◽

Her2 Breast Cancer

In this study, a magnetic core–shell modified tumor-targeting nanocarrier (MNPs-PEG–TRA) was engineered and demonstrated for the efficientin vitroandin vivohyperthermia treatment of breast cancer.

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In Vitro Trafficking and Efficacy of Core-Shell Nanostructures for Treating Intracellular Salmonella Infections

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00009-09 ◽

2009 ◽

Vol 53 (9) ◽

pp. 3985-3988 ◽

Cited By ~ 12

Author(s):

A. Ranjan ◽

N. Pothayee ◽

M. N. Seleem ◽

N. Sriranganathan ◽

R. Kasimanickam ◽

...

Keyword(s):

Flow Cytometry ◽

Confocal Microscopy ◽

Surface Property ◽

Core Shell ◽

Bacterial Clearance ◽

Bacterial Counts ◽

Shell Nanostructures ◽

Salmonella Infections

ABSTRACT Nanostructures encapsulating gentamicin and having either amphiphilic (N1) or hydrophilic (N2) surfaces were designed. Flow cytometry and confocal microscopy studies demonstrated a higher rate of uptake for amphiphilic surfaces. A majority of N1 were localized in the cytoplasm, whereas N2 colocalized with the endosomes/lysosomes. Colocalization was not observed between nanostructures and intracellular Salmonella bacteria. However, significant in vitro reductions in bacterial counts (0.44 log10) were observed after incubation with N1, suggesting that the surface property of the nanostructure influences intracellular bacterial clearance.

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