Bioglass 45S5: Structural characterization of short range order and analysis of biocompatibility with adipose-derived mesenchymal stromal cells in vitro and in vivo

Brown-like adipocytes can be induced in white fat depots by a different environmental or drug stimuli, known as “browning” or “beiging”. These brite adipocytes express thermogenin UCP1 protein and show different metabolic advantages, such as the ability to acquire a thermogenic phenotype corresponding to standard brown adipocytes that counteracts obesity. In this research, we evaluated the effects of several browning agents during white adipocyte differentiation of bone marrow-derived mesenchymal stromal cells (MSCs). Our in vitro findings identified two compounds that may warrant further in vivo investigation as possible anti-obesity drugs. We found that rosiglitazone and sildenafil are the most promising drug candidates for a browning treatment of obesity. These drugs are already available on the market for treating diabetes and erectile dysfunction, respectively. Thus, their off-label use may be contemplated, but it must be emphasized that some severe side effects are associated with use of these drugs.

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Transplantation of mesenchymal stromal cells on mineralized collagen leads to ectopic matrix synthesis in vivo independently from prior in vitro differentiation

Cytotherapy ◽

10.1080/14653240600845187 ◽

2006 ◽

Vol 8 (4) ◽

pp. 354-366 ◽

Cited By ~ 18

Author(s):

P. Niemeyer ◽

P. Kasten ◽

H.-G. Simank ◽

J. Fellenberg ◽

A. Seckinger ◽

...

Keyword(s):

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

In Vitro Differentiation ◽

Matrix Synthesis ◽

Mineralized Collagen

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Characterization of the immunomodulatory effect in vitro of microvesicles isolated from mesenchymal stromal cells on immune cells

Experimental Hematology ◽

10.1016/j.exphem.2013.05.249 ◽

2013 ◽

Vol 41 (8) ◽

pp. S64

Author(s):

Antonella Conforti ◽

Marco Scarsella ◽

Ezio Giorda ◽

Simone Biagini ◽

Nadia Starc ◽

...

Keyword(s):

Mesenchymal Stromal Cells ◽

Immune Cells ◽

Stromal Cells ◽

Immunomodulatory Effect

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Effect of extracorporeal shock wave therapy on equine umbilical cord blood mesenchymal stromal cells in vitro

10.1101/2020.01.10.901439 ◽

2020 ◽

Author(s):

Ramés Salcedo-Jiménez ◽

Judith Koenig ◽

Olivia Lee ◽

Thomas W.G. Gibson ◽

Pavneesh Madan ◽

...

Keyword(s):

Shock Wave ◽

Mesenchymal Stromal Cells ◽

Umbilical Cord Blood ◽

Stromal Cells ◽

Extracorporeal Shock Wave Therapy ◽

Shock Wave Therapy ◽

Extracorporeal Shock Wave ◽

Immunomodulatory Properties

AbstractExtracorporeal shock wave therapy (ESWT) has been shown to induce different biological effects on a variety of cells, including regulation and stimulation of their function and metabolism. ESWT can promote different biological responses such as proliferation, migration, and regenerations of cells. Recent studies have shown that mesenchymal stromal cells (MSCs) secrete factors that enhance the regeneration of tissues, stimulate proliferation and differentiation of cells and decrease inflammatory and immune-reactions. Clinically, the combination of these two therapies has been used as a treatment for tendon and ligament lesions in horses; however, there is no scientific evidence supporting this combination of therapies in vivo. Therefore, the objectives of the study were to evaluate the effects of ESWT on equine umbilical cord blood mesenchymal stromal cells (CB-MSCs) proliferative, metabolic, migrative, differentiation, and immunomodulatory properties in vitro. Three equine CB-MSC cultures from independent donors were treated using an electrohydraulic shock wave generator attached to a water bath. All experiments were performed as triplicates. Proliferation, viability, migration and immunomodulatory properties of the cells were evaluated. Equine CB-MSCs were induced to evaluate their trilineage differentiation potential. ESWT treated cells had increased metabolic activity, showed positive adipogenic, osteogenic, and chondrogenic differentiation, and showed higher potential for differentiation towards the adipogenic and osteogenic cell fates. ESWT treated cells showed similar immunomodulatory properties to none-ESWT treated cells. Equine CB-MSCs are responsive to ESWT treatment and showed increased metabolic, adipogenic and osteogenic activity, but unaltered immunosuppressive properties. In vivo studies are warranted to determine if synergistic effects occur in the treatment of musculoskeletal injuries if ESWT and equine CB-MSC therapies are combined.

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