Epithelial basement membrane of human decellularized cornea as a suitable substrate for differentiation of embryonic stem cells into corneal epithelial-like cells

2020 ◽  
Vol 116 ◽  
pp. 111215 ◽  
Author(s):  
Thaís Maria da Mata Martins ◽  
Pricila da Silva Cunha ◽  
Michele Angela Rodrigues ◽  
Juliana Lott de Carvalho ◽  
Joyce Esposito de Souza ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Basement Membrane ◽  
Embryonic Stem ◽  
Corneal Epithelial ◽  
Epithelial Basement Membrane ◽  
Suitable Substrate ◽  
Epithelial Basement

scholarly journals Assessment of corneal epithelial thickness mapping in epithelial basement membrane dystrophy

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0239124
Author(s):  
Juliette Buffault ◽  
Pierre Zéboulon ◽  
Hong Liang ◽  
Anthony Chiche ◽  
Jade Luzu ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Normal Subjects ◽  
Optical Quality ◽  
Quality Analysis ◽  
Corneal Epithelial ◽  
Epithelial Thickness ◽  
Epithelial Basement Membrane ◽  
The Mean ◽  
Corneal Epithelial Thickness ◽  
Epithelial Basement

Purpose To investigate the corneal epithelial thickness topography with optical coherence tomography (OCT) and its relationship with vision quality in epithelial basement membrane dystrophy (EBMD). Methods 45 eyes of EBMD patients, 26 eyes of dry eye (DED) patients and 22 eyes of normal subjects were enrolled. All participants were subjected to 9-mm corneal epithelial mapping with OCT and vision quality was assessed with the optical quality analysis system using the objective scatter index (OSI). Central, superior, inferior, minimum, maximum, and standard deviation of epithelium thickness (Irregularity), were analysed and correlations with the OSI were calculated. Results The mean (±SD) central, inferior and maximum epithelial thicknesses of the EBMD patients (respectively, 56.4 (±8.1) μm, 58.9 (±6.4) μm, and 67.1 (±8.3) μm) were thicker compared to DED patients (P<0.05) and normal subjects (P<0.05). We found greater irregularity of epithelial thickness in EBMD (5.1±2.5 μm) compared to DED patients (2.6±1.0 μm) (P = 4.4.10−6) and normal subjects (2.1±0.7 μm) (P = 7.6.10−7). The mean OSI was worse in EBMD patients than in DED patients (P = 0.01) and compared to normal subjects (P = 0.02). The OSI correlated with the epithelial thickness irregularity (Spearman coefficient = 0.54; P = 2.65.10−5). Conclusions The OCT pachymetry map demonstrated that EBMD patients had thicker corneal epithelium in the central and inferior region. These changes were correlated with objective measurements of vision quality. This OCT characterisation of the EMBD provides a better understanding of the epithelial behaviour in this dystrophy and its role in vision quality.

scholarly journals Human Corneal Epithelial Basement Membrane and Integrin Alterations in Diabetes and Diabetic Retinopathy1

1998 ◽  
Vol 46 (9) ◽  
pp. 1033-1041 ◽  
Author(s):  
Alexander V. Ljubimov ◽  
Zhi-shen Huang ◽  
Gang H. Huang ◽  
Robert E. Burgeson ◽  
Jeffrey H. Miner ◽  
...  
Keyword(s):  
Wound Healing ◽  
Basement Membrane ◽  
Normal Subjects ◽  
Diabetic Patients ◽  
Integrin Receptors ◽  
Corneal Epithelial ◽  
Epithelial Basement Membrane ◽  
Laminin Binding ◽  
Epithelial Basement ◽  
Laminin 1

Corneas of diabetic patients have abnormal healing and epithelial adhesion, which may be due to alterations of the corneal extracellular matrix (ECM) and basement membrane (BM). To identify such alterations, various ECM and BM components and integrin receptors were studied by immunofluorescence on sections of normal and diabetic human corneas. Age-matched corneas from 15 normal subjects, 10 diabetics without diabetic retinopathy (DR), and 12 diabetics with DR were used. In DR corneas, the composition of the central epithelial BM was markedly altered, compared to normal or non-DR diabetic corneas. In most cases the staining for entactin/nidogen and for chains of laminin-1 (α1β1γ1) and laminin-10 (α5β1γ1) was very weak, discontinuous, or absent over large areas. Other BM components displayed less frequent changes. The staining for α3β1 (VLA-3) laminin binding integrin was also weak and discontinuous in DR corneal epithelium. Components of stromal ECM remained unchanged even in DR corneas. Therefore, distinct changes were identified in the composition of the epithelial BM in DR corneas. They may be due to increased degradation or decreased synthesis of BM components and related integrins. These alterations may directly contribute to the epithelial adhesion and wound healing abnormalities found in diabetic corneas.

Absence of type IV collagen in the centre of the corneal epithelial basement membrane

1990 ◽  
Vol 22 (12) ◽  
pp. 688-694 ◽  
Author(s):  
J. P. M. Cleutjens ◽  
M. G. Havenith ◽  
M. Kasper ◽  
M. Vallinga ◽  
F. T. Bosman
Keyword(s):  
Basement Membrane ◽  
Type Iv Collagen ◽  
Corneal Epithelial ◽  
Epithelial Basement Membrane ◽  
Type Iv ◽  
Epithelial Basement

Map-Fingerprint-Dot Changes in the Corneal Epithelial Basement Membrane Following Radial Keratotomy

Ophthalmology ◽  
1985 ◽  
Vol 92 (2) ◽  
pp. 199-205 ◽  
Author(s):  
J. Daniel Nelson ◽  
Patricia Williams ◽  
Richard L. Lindstrom ◽  
Donald J. Doughman
Keyword(s):  
Basement Membrane ◽  
Corneal Epithelial ◽  
Radial Keratotomy ◽  
Epithelial Basement Membrane ◽  
Epithelial Basement

Spontaneous Chronic Corneal Epithelial Defects in Dogs: A Review

2005 ◽  
Vol 41 (3) ◽  
pp. 158-165 ◽  
Author(s):  
Ellison Bentley
Keyword(s):  
Wound Healing ◽  
Basement Membrane ◽  
Success Rate ◽  
Treatment Options ◽  
Middle Aged ◽  
Delayed Wound Healing ◽  
Corneal Epithelial ◽  
Histopathological Findings ◽  
Epithelial Basement Membrane ◽  
Epithelial Basement

Spontaneous chronic corneal epithelial defects (SCCEDs) in dogs are typically found in middle-aged dogs of all breeds. These epithelial defects may be present for weeks to months, particularly if left untreated or if treated inappropriately. Typical histopathological findings include loss of the corneal epithelial basement membrane and formation of a superficial, acellular, hyalinized zone in the stroma. Together, these histological abnormalities lead to delayed wound healing and poor epithelial adhesion. Epithelial debridement, anterior stromal puncture, grid keratotomy, and superficial keratectomy are the most common treatment options applied to the defects. Procedures that address the stromal changes present generally have a higher success rate than epithelial debridement alone.

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