Porphyra yezoensis polysaccharide and potassium citrate synergistically inhibit calcium oxalate crystallization induced by renal epithelial cells and cytotoxicity of the formed crystals

2021 ◽  
Vol 119 ◽  
pp. 111448
Author(s):  
Xin-Yuan Sun ◽  
Hui Zhang ◽  
Jia-Yun Chen ◽  
Guo-Hua Zeng ◽  
Jian-Ming Ouyang
Keyword(s):  
Epithelial Cells ◽  
Calcium Oxalate ◽  
Porphyra Yezoensis ◽  
Potassium Citrate ◽  
Renal Epithelial Cells ◽  
Calcium Oxalate Crystallization

scholarly journals Protective Effect of Degraded Porphyra yezoensis Polysaccharides on the Oxidative Damage of Renal Epithelial Cells and on the Adhesion and Endocytosis of Nanocalcium Oxalate Crystals

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Qian-Long Peng ◽  
Chuang-Ye Li ◽  
Yao-Wang Zhao ◽  
Xin-Yuan Sun ◽  
Hong Liu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Epithelial Cells ◽  
Oxidative Damage ◽  
Protective Effect ◽  
Calcium Oxalate ◽  
Kidney Stone ◽  
Stone Formation ◽  
Porphyra Yezoensis ◽  
Renal Epithelial Cells ◽  
Kidney Stone Formation

The protective effects of Porphyra yezoensis polysaccharides (PYPs) with molecular weights of 576.2 (PYP1), 105.4 (PYP2), 22.47 (PYP3), and 3.89 kDa (PYP4) on the oxidative damage of human kidney proximal tubular epithelial (HK-2) cells and the differences in adherence and endocytosis of HK-2 cells to calcium oxalate monohydrate crystals before and after protection were investigated. Results showed that PYPs can effectively reduce the oxidative damage of oxalic acid to HK-2 cells. Under the preprotection of PYPs, cell viability increased, cell morphology improved, reactive oxygen species levels decreased, mitochondrial membrane potential increased, S phase cell arrest was inhibited, the cell apoptosis rate decreased, phosphatidylserine exposure reduced, the number of crystals adhered to the cell surface reduced, but the ability of cells to endocytose crystals enhanced. The lower the molecular weight, the better the protective effect of PYP. The results in this article indicated that PYPs can reduce the risk of kidney stone formation by protecting renal epithelial cells from oxidative damage and reducing calcium oxalate crystal adhesion, and PYP4 with the lowest molecular weight may be a potential drug for preventing kidney stone formation.

Size-dependent toxicity and interactions of calcium oxalate dihydrate crystals on Vero renal epithelial cells

2015 ◽  
Vol 3 (9) ◽  
pp. 1864-1878 ◽  
Author(s):  
Xin-Yuan Sun ◽  
Jian-Ming Ouyang ◽  
Wen-Yu Zhu ◽  
Yu-Bao Li ◽  
Qiong-Zhi Gan
Keyword(s):  
Epithelial Cells ◽  
Calcium Oxalate ◽  
Calcium Oxalate Dihydrate ◽  
Renal Epithelial Cells ◽  
Size Dependent ◽  
Oxalate Dihydrate

Size-dependent toxicity and interactions of calcium oxalate dihydrate crystals on Vero renal epithelial cells were demonstrated.

cDNA macroarray analysis of genes in renal epithelial cells exposed to calcium oxalate crystals

2008 ◽  
Vol 37 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Katsuhito Miyazawa ◽  
Kinue Aihara ◽  
Ryosuke Ikeda ◽  
Manabu T. Moriyama ◽  
Koji Suzuki
Keyword(s):  
Epithelial Cells ◽  
Calcium Oxalate ◽  
Calcium Oxalate Crystals ◽  
Renal Epithelial Cells ◽  
Cdna Macroarray ◽  
Oxalate Crystals ◽  
Macroarray Analysis

scholarly journals Calcium oxalate monohydrate crystals stimulate gene expression in renal epithelial cells

1995 ◽  
Vol 48 (2) ◽  
pp. 501-509 ◽  
Author(s):  
Mary S. Hammes ◽  
John C. Lieske ◽  
Shashi Pawar ◽  
Benjamin H. Spargo ◽  
F. Gary Toback
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Calcium Oxalate ◽  
Calcium Oxalate Monohydrate ◽  
Renal Epithelial Cells
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