Chitosan-poloxamer-based thermosensitive hydrogels containing zinc gluconate/recombinant human epidermal growth factor benefit for antibacterial and wound healing

Abstract Background Vacuum sealing drainage (VSD) and epidermal growth factor (EGF) both play an important role in the treatment of wounds. This study aims to explore the effects of the combination of VSD and EGF on wound healing and the optimal concentration and time of EGF. Methods We tested the proliferation and migration capacity of HaCaT and L929 cells at different EGF concentrations (0, 1, 5, 10, and 100 ng/ml) and different EGF action times (2, 10, and 30 min). A full-thickness skin defect model was established using male, 30-week-old Bama pigs. The experiment included groups as follows: routine dressing change after covering with sterile auxiliary material (Control), continuous negative pressure drainage of the wound (VSD), continuous negative pressure drainage of the wound and injection of EGF 10 min followed by removal by continuous lavage (V + E 10 min), and continuous negative pressure drainage of the wound and injection of EGF 30 min followed by removal by continuous lavage (V + E 30 min). The wound healing rate, histological repair effect and collagen deposition were compared among the four groups. Results An EGF concentration of 10 ng/ml and an action time of 10 min had optimal effects on the proliferation and migration capacities of HaCaT and L929 cells. The drug dispersion effect was better than drug infusion after bolus injection effect, and the contact surface was wider. Compared with other groups, the V + E 10 min group promoted wound healing to the greatest extent and obtained the best histological score. Conclusions A recombinant human epidermal growth factor (rhEGF) concentration of 10 ng/ml can promote the proliferation and migration of epithelial cells and fibroblasts to the greatest extent in vitro. VSD combined with rhEGF kept in place for 10 min and then washed, can promote wound healing better than the other treatments in vivo.

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Chitosan-Based Composite Nanosilver Gel Application in Nursing Care of Intensive Care Unit Patients with Severe Burns

Nanoscience and Nanotechnology Letters ◽

10.1166/nnl.2020.3192 ◽

2020 ◽

Vol 12 (7) ◽

pp. 939-945

Author(s):

Ningning Rong ◽

Ruyan Yang ◽

Yongzan Lu

Keyword(s):

Wound Healing ◽

Growth Factor ◽

Epidermal Growth Factor ◽

High Efficiency ◽

Tissue Cell ◽

Petroleum Jelly ◽

Burn Wound ◽

Human Epidermal Growth Factor ◽

Severe Burns ◽

Epidermal Growth

Burns are the most common clinical disease in daily life. Burn wounds are vulnerable to bacterial infection due to factors such as edema, hypoxia, and ischemia. However, traditional antibacterial supplements do not achieve satisfactory nursing results. Here, we use chitosan as a carrier by introducing silver nanoparticles (AgNPs) and recombinant human epidermal growth factor (rhEGF) together. We have constructed a new type of high moisture retention, high oxygen permeability, high-efficiency chitosan-nanosilver@recombinant human epidermal growth factor (CSAgNPs@ rhEGF) antibacterial hydrogel dressing. The clinical trials showed that the antibacterial effect of CS-AgNPs@rhEGF hydrogel dressings against Pseudomonas aeruginosa (Pa.) and Staphylococcus epidermidis (Se.) was significantly better than that of petroleum jelly and 1% silver sulfadiazine (1%-SSD). In addition, rhEGF promoted tissue cell proliferation and repair. Thus, the CS-AgNPs@rhEGF hydrogel dressing can significantly improve the wound healing rate and healing time in patients with severe burns compared with traditional dressings. Thus, CS-AgNPs@rhEGF hydrogel dressing is expected to provide a new choice for clinical burn patients to prevent wound infection and tissue repair, and has a broad prospect in burn wound healing.

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