Chronic effects of GM soybean oil on some semen characteristics of rainbow trout (Onchorhyncus mykiss W., 1792) in breeding season

2012 ◽  
Vol 29 ◽  
pp. S193
Author(s):  
Zafer Doğu ◽  
Erdinç Şahinöz ◽  
Faruk Aral
1977 ◽  
Vol 34 (5) ◽  
pp. 677-682 ◽  
Author(s):  
J. F. Leatherland ◽  
C. Y. Cho ◽  
S. J. Slinger

Rainbow trout (Salmo gairdneri) acclimated to 19 °C and fed a pelleted diet of either 6% soybean oil or 6% tallow fat had similar plasma thyroxine levels (0.62 ± 0.10 and 0.69 ± 0.08 μg/100 ml, respectively), although fish acclimated to 7 °C and fed the soybean diet gave significantly (P < 0.05) higher plasma thyroxine concentration (1.32 ± 0.13 μg/100 ml) than the group fed the tallow fat diet (0.95 ± 0.12 μg/100 ml). The plasma thyroxine concentration in the fish fed the soybean oil diet was significantly (P < 0.01) higher at 7 °C than at 19 °C. There was no significant difference between the two groups of fish given the tallow fat diet. Epithelial cell heights were largest in groups with the highest plasma thyroxine levels. Epithelial cell height in the two groups at 19 °C were similar, but in both groups of fish at 7 °C, the cell height was significantly greater than in either of the groups at 19 °C (P < 0.01 for comparisons of the 19 °C tallow fat diet with both groups of 7 °C acclimated fish and comparison of the two groups on the soybean oil diet; P < 0.05 for comparisons of the 19 °C, soybean oil diet with the group held at 7 °C and given the tallow fat diet). Fish deprived of food for 40 days had a significantly (P < 0.05) lower plasma thyroxine concentration when compared with fish that had been fed daily. There was no significant diurnal variation in plasma thyroxine over the light phase of a 12 h light: 12 h dark photoperiod, nor did the limitation of light entering the aquaria significantly affect plasma thyroxine values. Key words: endocrine, thyroid, Salmo gairdneri, diet, environmental temperature


2009 ◽  
Vol 21 (9) ◽  
pp. 35
Author(s):  
A. Towhidi ◽  
A. Farshad ◽  
M. Dolatpanah ◽  
R. Salehi

The aim of the study was to investigate the effect of dietary supplementation of vitamin E on the semen characteristics of the Markhoz goats. Eighth bucks were randomly allocated into two groups, and received two different diets: unsupplemented diet (control) and supplemented diet with vitamin E (0.30 g/Kg DM). All experimental diets were formulated according to AFRC 1998. Semen was collected at 14-days intervals from June 17, 2006 to September 2, 2006 (non-breeding season) using artificial vagina. Semen characteristics were evaluated including semen volume, sperm concentration, live sperm percentage, percentage of motility and progressive motility. This characteristics were evaluated at two weeks intervals the trial. The total number of spermatozoa per ejaculation was calculated by multiplication of the semen volume with sperm concentration. Sperm motility was also analyzed by placing a sample on pre-warmed (37 º C) microscope slide covered with a cover slip, and examined under a high power microscope at a magnification × 200. Data was analyzed using proc MIXED in SAS program. Significant effect (P≤0.05) of the week (sampling time) was observed for all the parameters except for semen volume. Vitamin E supplementation significantly improved (P≤0.05) total number (Control: 263.7 ×107±17.506 vs. Vit E: 320.95 ×107±17.506) and sperm concentration (Control: 301.79 ×107±13.657 vs. Vit E: 386.57 ×107± 13.657), motility (Control: 77.27% ±0.89 vs. Vit E: 82.6% ±0.89) and progressive motility (Control: 4.208 ±0.138 vs. Vit E: 4.229 ±0.138), percentage of viability (Control: 80.57% ±0.89 vs. Vit E: 85.9% ±0.89). The results suggested that the supplemental Vitamin E may improve the semen quality and fertility in the Markhoz goats.


2009 ◽  
Vol 57 (4) ◽  
pp. 531-540 ◽  
Author(s):  
Vera Faigl ◽  
Mónika Keresztes ◽  
Margit Kulcsár ◽  
Sándor Nagy ◽  
Zsuzsanna Keresztes ◽  
...  

The objective of this study was to evaluate the effect of long-term melatonin treatment applied during the non-breeding season on semen characteristics, endocrine function of testicles and baseline level of insulin-like growth factor-I (IGF-I) in Awassi rams kept in the temperate continental zone of Europe and used as semen donors in an artificial insemination (AI) programme. On 23 February (day 0), slow-release melatonin implants were inserted subcutaneously into rams (n = 8). Control animals (n = 8) received no treatment. In both groups, basic semen parameters (concentration, total motility, fast and slow forward motility, morphology), GnRH-induced testosterone response and basal IGF-I concentration were evaluated on days 0, 47 and 71. No differences were found in concentration of spermatozoa, total motility, and numbers of spermatozoa with fast and slow progressive motility and normal/abnormal morphology between the melatonin-treated and the control group. However, in melatonin-treated animals, basal and GnRH-induced testosterone levels were slightly elevated on day 47 and became significantly higher on day 71 (P < 0.05) as compared to controls. There was no difference in plasma IGF-I levels between the groups. In conclusion, slow-release melatonin applied during the non-breeding season improves testicular testosterone production but does not influence the semen characteristics and the IGF-I level of semen donor Awassi rams used in an AI programme and kept in the temperate continental zone of Europe.


2020 ◽  
Vol 89 (3) ◽  
pp. 291-300
Author(s):  
Vladimír Piaček ◽  
Jan Zukal ◽  
Veronika Seidlová ◽  
Tomáš Heger ◽  
Monika Němcová ◽  
...  

Artificial insemination (AI) is the most frequently used assisted reproductive technique for captive propagation of rare avian species. As semen quality is critical for reproductive success, baseline data are needed for evaluating and selecting the best male bird donors. To this end, we used computer-assisted semen analysis to assess male eastern imperial eagles (n = 7), northern goshawks (n = 24) and peregrine falcons (n = 20). While imperial eagles and northern goshawks donate ejaculate voluntarily, peregrine falcons required cloacal massage. Eight peregrine falcon females were inseminated with semen from eight males, with fresh ejaculates (15 to 50 µl) applied to the pars uterina of the oviduct immediately after collection and examination. All females were inseminated within 2 h of laying an egg. A fertilization rate of 70% was achieved using this method. Minimum semen characteristics associated with egg fertilization included a semen concentration of 115.12 × 106/ml, 33.52% total motility, 1.92% spermatozoa with progressive motility and 0.17% with rapid motility. Comparative data on spermatozoa concentration and kinematics suggest that eastern imperial eagles concentrate on high quality semen investment at the start of the breeding season, northern goshawks compensate for a decrease in motility-associated parameters with increased semen concentration and peregrine falcons maintain semen production standards throughout the breeding season. Our data show that, in birds of prey, levels of egg fertilization following AI with fresh semen can be almost as successful as after natural mating.


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