Rhythmic post-transcriptional regulation of the circadian clock protein mPER2 in mammalian cells: A real-time analysis

2006 ◽  
Vol 401 (1-2) ◽  
pp. 44-48 ◽  
Author(s):  
Keigo Nishii ◽  
Iori Yamanaka ◽  
Maya Yasuda ◽  
Yota B. Kiyohara ◽  
Yoko Kitayama ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Circadian Clock ◽  
Real Time ◽  
Mammalian Cells ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Post Transcriptional Regulation ◽  
Clock Protein

Real-time analysis of the transcriptional regulation of HIV and hCMV promoters in single mammalian cells

1995 ◽  
Vol 108 (2) ◽  
pp. 441-455
Author(s):  
M.R. White ◽  
M. Masuko ◽  
L. Amet ◽  
G. Elliott ◽  
M. Braddock ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Sodium Butyrate ◽  
Mammalian Cells ◽  
Viral Gene Expression ◽  
Luciferase Expression ◽  
Viral Gene ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Viral Promoters

The regulation of human cytomegalovirus (hCMV) and human immunodeficiency virus (HIV) gene expression has been studied in single intact mammalian cells. Viral promoters were placed upstream of the firefly luciferase reporter gene and the resulting hybrid reporter constructs were stably integrated into the HeLa cell genome. A highly sensitive photon-counting camera system was used to study the level of gene expression in single intact cells. Luciferase expression was studied in the absence of activators of viral gene expression, in the presence of the HIV-1 TAT transactivator protein, or in the presence of sodium butyrate, a non-viral activator of gene expression. In the absence of any activator of gene expression, while expression was undetectable in most cells, significant levels of basal luciferase activity were observed in a few cells, indicating heterogeneity in gene expression in the cell population. In the presence of the general activator of viral gene expression, sodium butyrate, transcriptional activation from the viral promoters gave rise to significant and relatively homogeneous levels of luciferase expression in a majority of cells. The luciferase imaging technology was used for the real-time analysis of changes of gene expression within a single cell. This non-invasive reporter assay should become important for studies of the temporal regulation of gene expression in single cells.

An Interactive Minicomputer Program for the Indexing and Simulation of Electron Diffraction Patterns

1976 ◽  
Vol 34 ◽  
pp. 542-543
Author(s):  
R.P. Goehner ◽  
W.T. Hatfield ◽  
Prakash Rao
Keyword(s):  
Electron Diffraction ◽  
Real Time ◽  
Pattern Analysis ◽  
Flow Diagram ◽  
Hard Copy ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Transmission Electron ◽  
One Step ◽  
Diffraction Patterns

Computer programs are now available in various laboratories for the indexing and simulation of transmission electron diffraction patterns. Although these programs address themselves to the solution of various aspects of the indexing and simulation process, the ultimate goal is to perform real time diffraction pattern analysis directly off of the imaging screen of the transmission electron microscope. The program to be described in this paper represents one step prior to real time analysis. It involves the combination of two programs, described in an earlier paper(l), into a single program for use on an interactive basis with a minicomputer. In our case, the minicomputer is an INTERDATA 70 equipped with a Tektronix 4010-1 graphical display terminal and hard copy unit.A simplified flow diagram of the combined program, written in Fortran IV, is shown in Figure 1. It consists of two programs INDEX and TEDP which index and simulate electron diffraction patterns respectively. The user has the option of choosing either the indexing or simulating aspects of the combined program.

scholarly journals Real-Time Analysis of Animal Feeding Behavior With a Low-Calculation-Power CPU

2020 ◽  
Vol 67 (4) ◽  
pp. 1197-1205 ◽  
Author(s):  
Yuki Totani ◽  
Susumu Kotani ◽  
Kei Odai ◽  
Etsuro Ito ◽  
Manabu Sakakibara
Keyword(s):  
Feeding Behavior ◽  
Real Time ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Animal Feeding

A threshold-based, real-time analysis in early detection of endpoint anomalies using SIEM expertise

2021 ◽  
Vol 2021 (4) ◽  
pp. 7-16
Author(s):  
Sivaraman Eswaran ◽  
Aruna Srinivasan ◽  
Prasad Honnavalli
Keyword(s):  
Early Detection ◽  
Real Time ◽  
Time Analysis ◽  
Real Time Analysis

Chromo-fluorogenic sensors for chemical warfare agents in real-time analysis: journey towards accurate detection and differentiation

2021 ◽  
Vol 57 (28) ◽  
pp. 3430-3444
Author(s):  
Vinod Kumar
Keyword(s):  
Real Time ◽  
Chemical Warfare Agents ◽  
Chemical Warfare ◽  
Chemical Probes ◽  
Time Analysis ◽  
Specific Detection ◽  
Success Stories ◽  
Real Time Analysis ◽  
Warfare Agents ◽  
Unique Chemical

This article describes our journey and success stories in the development of chemical warfare detection, detailing the range of unique chemical probes and methods explored to achieve the specific detection of individual agents in realistic environments.

scholarly journals A Framework for International Collaboration on ITER Using Large-Scale Data Transfer to Enable Near-Real-Time Analysis

2021 ◽  
Vol 77 (2) ◽  
pp. 98-108
Author(s):  
R. M. Churchill ◽  
C. S. Chang ◽  
J. Choi ◽  
J. Wong ◽  
S. Klasky ◽  
...  
Keyword(s):  
Real Time ◽  
International Collaboration ◽  
Large Scale ◽  
Data Transfer ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Large Scale Data ◽  
Scale Data
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