Susceptibility to oxygen-glucose deprivation is reduced in acute hippocampal slices from euthermic Syrian golden hamsters relative to slices from Sprague-Dawley rats

2013 ◽  
Vol 553 ◽  
pp. 13-17 ◽  
Author(s):  
John G. Mielke
Keyword(s):  
Hippocampal Slices ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Sprague Dawley ◽  
Golden Hamsters ◽  
Sprague Dawley Rats ◽  
Syrian Golden Hamsters ◽  
Acute Hippocampal Slices

D-Glucose Combined Chronic Toxicity and Carcinogenicity Studdjs in Sprague-Dawley Rats and Syrian Golden Hamsters

1998 ◽  
Vol 21 (3) ◽  
pp. 329-353 ◽  
Author(s):  
E. Bomhard ◽  
H. Bischoff ◽  
H. Mager ◽  
F. Krötlinger ◽  
B. Schilde
Keyword(s):  
Chronic Toxicity ◽  
Sprague Dawley ◽  
Golden Hamsters ◽  
Sprague Dawley Rats ◽  
Syrian Golden Hamsters

Differences in acute lung response to elastase instillation in two rodent species may determine differences in severity of emphysema development

2011 ◽  
Vol 301 (1) ◽  
pp. R148-R158 ◽  
Author(s):  
Andrea Vecchiola ◽  
Juan Francisco de la Llera ◽  
Rodrigo Ramírez ◽  
Pablo Olmos ◽  
Cristobal I. Herrera ◽  
...  
Keyword(s):  
Species Differences ◽  
Capillary Permeability ◽  
Fold Increase ◽  
Sprague Dawley ◽  
Golden Hamsters ◽  
Sprague Dawley Rats ◽  
Syrian Golden Hamsters ◽  
Tnf Α ◽  
Lung Response ◽  
Alveolar Capillary

Elastase intratracheal instillation induces early emphysema in rodents. However, Syrian Golden hamsters develop more severe emphysema than Sprague-Dawley rats. We have reported species differences in oxidant/antioxidant balance modulating antiprotease function early after instillation. We now hypothesize that other components of the initial lung response to elastase might also be species-dependent. Sprague-Dawley rats and Syrian Golden hamsters received a single dose of pancreatic elastase (0.55 U/100 g body wt) to study acute lung injury biomarkers. Using serum, lung, and bronchoalveolar lavage fluid (BALF) samples, we evaluated changes in alveolar-capillary permeability, alpha 1-antitrypsin (α1-AT) concentration and activity, glutathione content, and proinflammatory cytokines. Rats showed a large increase in alveolar-capillary permeability and few hemorrhagic changes, whereas hamsters exhibited large hemorrhagic changes ( P < 0.01) and mild transendothelial passage of proteins. Western blots showed a 30-fold increase in BALF α1-AT concentration in rats and only a 7-fold increase in hamsters ( P < 0.001), with [α1-AT-elastase] complexes only in rats, suggesting differences in antiprotease function. This was confirmed by the α1-AT bioassay showing 20-fold increase in α1-AT activity in rats and only twofold increase in hamsters ( P < 0.001). In rats, results were preceded by a 3-, 60-, and 20-fold increase in IL-6, IL-1β, and TNF-α respectively ( P < 0.001). In hamsters, only IL-1β and TNF-α showed mild increases. All parameters studied were back to baseline by 4 days. In conclusion, several components of the initial lung response showed species differences. Cytokine release pattern and functional inhibition of α1-AT were the most significant components differing among species and could account for differences in susceptibility to elastase.

A chronic study of praziquantel in syrian golden hamsters and Sprague-Dawley rats

Toxicology ◽  
1982 ◽  
Vol 24 (3-4) ◽  
pp. 345-350 ◽  
Author(s):  
Madhukar Ketkar ◽  
Jürgen Althoff ◽  
Ulrich Mohr
Keyword(s):  
Sprague Dawley ◽  
Golden Hamsters ◽  
Sprague Dawley Rats ◽  
Syrian Golden Hamsters ◽  
Chronic Study

scholarly journals Arctic Ground Squirrel (Spermophilus Parryii) Hippocampal Neurons Tolerate Prolonged Oxygen—Glucose Deprivation and Maintain Baseline ERK1/2 and JNK Activation Despite Drastic ATP Loss

2008 ◽  
Vol 28 (7) ◽  
pp. 1307-1319 ◽  
Author(s):  
Sherri L Christian ◽  
Austin P Ross ◽  
Huiwen W Zhao ◽  
Heidi J Kristenson ◽  
Xinhua Zhan ◽  
...  
Keyword(s):  
Cell Death ◽  
Hippocampal Slices ◽  
Glucose Deprivation ◽  
Mapk Signaling ◽  
Ground Squirrels ◽  
Atp Depletion ◽  
Mitogen Activated Protein Kinase ◽  
Oxygen Glucose Deprivation ◽  
Acute Hippocampal Slices ◽  
Jnk Activation

Oxygen—glucose deprivation (OGD) initiates a cascade of intracellular responses that culminates in cell death in sensitive species. Neurons from Arctic ground squirrels (AGS), a hibernating species, tolerate OGD in vitro and global ischemia in vivo independent of temperature or torpor. Regulation of energy stores and activation of mitogen-activated protein kinase (MAPK) signaling pathways can regulate neuronal survival. We used acute hippocampal slices to investigate the role of ATP stores and extracellular signal-regulated kinase (ERK)1/2 and Jun NH2-terminal kinase (JNK) MAPKs in promoting survival. Acute hippocampal slices from AGS tolerated 30 mins of OGD and showed a small but significant increase in cell death with 2 h OGD at 37 C. This tolerance is independent of hibernation state or season. Neurons from AGS survive OGD despite rapid ATP depletion by 3 mins in interbout euthermic AGS and 10 mins in hibernating AGS. Oxygen—glucose deprivation does not induce JNK activation in AGS and baseline ERK1/2 and JNK activation is maintained even after drastic depletion of ATP. Surprisingly, inhibition of ERK1/2 or JNK during OGD had no effect on survival, whereas inhibition of JNK increased cell death during normoxia. Thus, protective mechanisms promoting tolerance to OGD by AGS are downstream from ATP loss and are independent of hibernation state or season.

scholarly journals Adenosine A1 receptor-mediated protection of mouse hippocampal synaptic transmission against oxygen and/or glucose deprivation: a comparative study

2019 ◽  
Vol 122 (2) ◽  
pp. 721-728 ◽  
Author(s):  
Masahito Kawamura ◽  
David N. Ruskin ◽  
Susan A. Masino
Keyword(s):  
Synaptic Transmission ◽  
Hippocampal Slices ◽  
Glucose Deprivation ◽  
Acute Response ◽  
Recording Conditions ◽  
Receptor Inactivation ◽  
Acute Hippocampal Slices ◽  
Metabolic Stresses ◽  
The Impact

Adenosine receptors are widely expressed in the brain, and adenosine is a key bioactive substance for neuroprotection. In this article, we clarify systematically the role of adenosine A1 receptors during a range of timescales and conditions when a significant amount of adenosine is released. Using acute hippocampal slices obtained from mice that were wild type or null mutant for the adenosine A1 receptor, we quantified and characterized the impact of varying durations of experimental ischemia, hypoxia, and hypoglycemia on synaptic transmission in the CA1 subregion. In normal tissue, these three stressors rapidly and markedly reduced synaptic transmission, and only treatment of sufficient duration led to incomplete recovery. In contrast, inactivation of adenosine A1 receptors delayed and/or lessened the reduction in synaptic transmission during all three stressors and reduced the magnitude of the recovery significantly. We reproduced the responses to hypoxia and hypoglycemia by applying an adenosine A1 receptor antagonist, validating the clear effects of genetic receptor inactivation on synaptic transmission. We found activation of adenosine A1 receptor inhibited hippocampal synaptic transmission during the acute phase of ischemia, hypoxia, or hypoglycemia and caused the recovery from synaptic impairment after these three stressors using genetic mutant. These studies quantify the neuroprotective role of the adenosine A1 receptor during a variety of metabolic stresses within the same recording system. NEW & NOTEWORTHY Deprivation of oxygen and/or glucose causes a rapid adenosine A1 receptor-mediated decrease in synaptic transmission in mouse hippocampus. We quantified adenosine A1 receptor-mediated inhibition during and synaptic recovery after ischemia, hypoxia, and hypoglycemia of varying durations using a genetic mutant and confirmed these findings using pharmacology. Overall, using the same recording conditions, we found the acute response and the neuroprotective ability of the adenosine A1 receptor depended on the type and duration of deprivation event.

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