Peripheral blood monocytes show morphological pattern of activation and decreased nitric oxide production during acute Chagas’ disease in rats

Nitric Oxide ◽  
2004 ◽  
Vol 11 (2) ◽  
pp. 166-174 ◽  
Author(s):  
Daniela L. Fabrino ◽  
Leonor L. Leon ◽  
Gleydes G. Parreira ◽  
Marcelo Genestra ◽  
Patrícia E. Almeida ◽  
...  
Nutrition ◽  
2007 ◽  
Vol 23 (7-8) ◽  
pp. 575-581 ◽  
Author(s):  
George Kolios ◽  
Katerina Kotzampassi ◽  
Pinelopi Manousou ◽  
Daniel Paramythiotis ◽  
Helen Papanastasiou ◽  
...  

2006 ◽  
Vol 20 (1) ◽  
pp. 40-46 ◽  
Author(s):  
Cathy J. Walsh ◽  
Jason D. Toranto ◽  
C. Taylor Gilliland ◽  
David R. Noyes ◽  
Ashby B. Bodine ◽  
...  

1994 ◽  
Vol 86 (4) ◽  
pp. 411-415 ◽  
Author(s):  
Helen F. Goode ◽  
Nigel R. Webster ◽  
Peter D. Howdle ◽  
Barry E. Walker

1. We describe a rapid and reliable technique for the assessment of basal nitric oxide release in clinical situations, using peripheral blood polymorphonuclear leucocytes isolated by a single-step density gradient procedure. The assay is based on the quantitative conversion of oxyhaemoglobin to methaemoglobin by nitric oxide. We have further examined the ability of these cells to respond to various stimuli. 2. Basal (unstimulated) nitric oxide release occurred, which was augmented by superoxide dismutase. The mean value for healthy subjects was 283 ±96.7 pmolmin−1 10−6 cells. 3. Both phorbol myristate acetate and N-formyl-methionyl-leucylphenylalanine induced further release of nitric oxide, which was increased by preincubation with lipopolysaccharide, interleukin-6 and interferon-γ. 4. Preincubation of cells with NG-monomethyl-l-arginine or l-canavanine sulphate inhibited nitric oxide production. 5. The procedure provides a valuable tool for monitoring nitric oxide up-regulation in clinical situations.


2003 ◽  
Vol 9 (S02) ◽  
pp. 1408-1409
Author(s):  
Daniela L. Fabrino ◽  
Leonor L. Leon ◽  
Gleydes G. Parreira ◽  
Patrícia E. Almeida ◽  
Rossana C.N. Melo

2001 ◽  
Vol 109 (06) ◽  
pp. 340-344 ◽  
Author(s):  
L. Schurman ◽  
C. Sedlinsky ◽  
A. Mangano ◽  
L. Sen ◽  
S. Leiderman ◽  
...  

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