Urinary organic acids as biomarkers in the assessment of pulmonary function in children with asthma

2019 ◽  
Vol 61 ◽  
pp. 31-40 ◽  
Author(s):  
Maria Michelle Papamichael ◽  
Charis Katsardis ◽  
Bircan Erbas ◽  
Catherine Itsiopoulos ◽  
Dimitris Tsoukalas
2003 ◽  
Vol 16 (4) ◽  
pp. 305-317 ◽  
Author(s):  
Ana P. Martinez-Donate ◽  
Melbourne F. Hovell ◽  
Dennis R. Wahlgren ◽  
Susan B. Meltzer ◽  
Eli O. Meltzer ◽  
...  

2012 ◽  
Vol 24 (3) ◽  
pp. 472-489 ◽  
Author(s):  
Alison Crosbie

ObjectiveAsthma is a leading cause of chronic illness in children, impacting heavily on their daily life and participation in physical activity. The purpose of this systematic review was to investigate the evidence for the use of physical therapy to improve pulmonary function and aerobic capacity in children with asthma. Furthermore, the review aims to update previous literature on the effect of exercise on health related quality of life.MethodsA search was conducted for randomized control trials (RCTs) using the electronic databases Medline, Embase, SPORTDiscus, AMED, CINAHL, and The Cochrane Central Register of Controlled Trials. Studies were included if the participants were asthmatic children aged 6–18 years participating in any mode of physical exercise. Studies were reviewed for study quality, participant details, exercise intervention details, and intervention outcomes.ResultsA total of 16 studies and 516 subjects met inclusion criteria for review. Severity of asthma ranged from mild to severe. No improvement in pulmonary function was observed. Physical training led to an increase in aerobic capacity as measured by VO2max (mL/kg/min).ConclusionsFindings suggest that physical training does not improve pulmonary function in children with asthma, but does increase aerobic capacity. The small number of studies investigating quality of life suggests that physical training does improve health related quality of life; however further well designed randomized control trials are needed to verify these findings.


1996 ◽  
Vol 42 (10) ◽  
pp. 1609-1615 ◽  
Author(s):  
P Duez ◽  
A Kumps ◽  
Y Mardens

Abstract We assessed the quantitative performances of a classical method for profiling urinary organic acids: ethyl acetate extraction/oxime-trimethylsilyl derivatization/GC-MS. Twenty-seven acids were quantified on the basis of specific ions in both scan and selected-ion monitoring modes. We found that the tuning of the mass detector severely affects the calibration factors, being critical to achieve quantitative results, and we propose a practical procedure for reproducible tuning. Of seven compounds tested, tropic acid was retained as the internal standard suitable for most of the acids of clinical interest; a second internal standard, 2-ketocaproic acid, was used in quantifying keto-acids. The within-day and total relative standard deviations (CVs), estimated from scan-mode analyses of urine, ranged from 2.6% to 12.7% and from 4.2% to 11.8%, respectively. Curvilinear relationships between analytical response and concentration were observed for most of the acids investigated.


1980 ◽  
Vol 26 (13) ◽  
pp. 1847-1853 ◽  
Author(s):  
K Tanaka ◽  
A West-Dull ◽  
D G Hine ◽  
T B Lynn ◽  
T Lowe

Abstract A gas-chromatographic method for urinary organic acid analysis is described, designed to be used routinely for the diagnosis of organic aciduria. It involves extraction of urine with ethyl acetate, dehydration of extract residues, and trimethylsilylation. Organic acids are identified by using an extensive list of retention indices published in the accompanying paper (this issue). Quantitative values are given for organic acids in urines from 50 ostensibly normal subjects. Typical chromatograms of urinary organic acids from patients with eight well-established organic acidurias are also shown.


1987 ◽  
Vol 33 (4) ◽  
pp. 572-576 ◽  
Author(s):  
B A Chamberlin ◽  
C C Sweeley

Abstract We evaluated for reliability and reproducibility a semiquantitative gas-chromatographic assay of organic acids in samples of normal urine recovered from absorbent filter paper. We also evaluated this method for use in diagnosis of some of the more common organic acidurias. Transfer of urine from diapers to absorbent filter paper eases the usual trauma of specimen collection from young children; it also simplifies sample storage and shipment.


2020 ◽  
Vol 56 (1) ◽  
pp. 34-41
Author(s):  
Lara Farhat ◽  
Gabriele Vos ◽  
Aliva De ◽  
Diana S. Lee ◽  
Deepa Rastogi

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