Expression and characterization of 15N-labeled human C-reactive protein in Escherichia coli and Pichia pastoris for use in isotope-dilution mass spectrometry

2012 ◽  
Vol 85 (1) ◽  
pp. 94-99 ◽  
Author(s):  
Eric L. Kilpatrick ◽  
Wei-Li Liao ◽  
Johanna E. Camara ◽  
Illarion V. Turko ◽  
David M. Bunk
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Pichia Pastoris ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry ◽  
C Reactive Protein ◽  
Reactive Protein

Development of a primary reference material of natural C-reactive protein: verification of its natural pentameric structure and certification by two isotope dilution mass spectrometry

2021 ◽  
Vol 13 (5) ◽  
pp. 626-635
Author(s):  
Jianyi Liu ◽  
Wen Zhu ◽  
Haofeng Sun ◽  
Dewei Song ◽  
Peng Xiao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Reference Material ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Primary Reference

Development of a primary reference material of natural C-reactive protein: verification of its natural pentameric structure and certification by two isotope dilution mass spectrometry.

Thermal-ionization isotope-dilution mass spectrometry as a definitive method for determination of potassium in serum.

1982 ◽  
Vol 28 (6) ◽  
pp. 1309-1313 ◽  
Author(s):  
J W Gramlich ◽  
L A Machlan ◽  
K A Brletic ◽  
W R Kelly
Keyword(s):  
Mass Spectrometry ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry ◽  
Accurate Method ◽  
Systematic Errors ◽  
Thermal Ionization ◽  
Mass Spectrometers ◽  
Definitive Method

Abstract Thermal-ionization isotope-dilution mass spectrometry is a highly precise and accurate method for the determination of potassium concentrations in serum. Although not suited for routine use because of the time and expense required, the technique provides an extremely valuable tool for the characterization of reference materials and for evaluating other analytical methods. The technique has recently been used to determine the concentration of potassium in a human serum standard, NBS Standard Reference Material 909. Seven vials of the serum were chemically processed and then analyzed by two spectroscopists independently, using different mass spectrometers. The results confirm previous work that indicates that a precision of 0.1% relative can be routinely achieved. The systematic errors in the method have been thoroughly evaluated. When the precise results are thus corrected, they are essentially bias free and hence definitive.

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