The JH-Met2-Kr-h1 pathway is involved in pyriproxyfen-induced defects of metamorphosis and silk protein synthesis in silkworms, Bombyx mori

2021 ◽  
pp. 104980
Author(s):  
Guoli Li ◽  
Huangli Lan ◽  
Qingyu Lu ◽  
Chunhui He ◽  
Yuting Wei ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Bombyx Mori ◽  
Silk Protein ◽  
Induced Defects

Effect of BBX-B8 overexpression on development, body weight, silk protein synthesis and egg diapause of Bombyx mori

2016 ◽  
Vol 25 (4) ◽  
pp. 507-516 ◽  
Author(s):  
Xiaojian Zheng ◽  
Yongchang Gong ◽  
Dhiraj Kumar ◽  
Fei Chen ◽  
Sulan Kuan ◽  
...  
Keyword(s):  
Body Weight ◽  
Protein Synthesis ◽  
Bombyx Mori ◽  
Silk Protein ◽  
Egg Diapause

scholarly journals Destruxin A Interacts with Aminoacyl tRNA Synthases in Bombyx mori

2021 ◽  
Vol 7 (8) ◽  
pp. 593
Author(s):  
Jingjing Wang ◽  
Alexander Berestetskiy ◽  
Qiongbo Hu
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Bombyx Mori ◽  
Metarhizium Anisopliae ◽  
Free Amino ◽  
Molecular Target ◽  
Trna Synthetases ◽  
Interaction Mode ◽  
Aminoacyl Trna Synthetases ◽  
Wide Range

Destruxin A (DA), a hexa-cyclodepsipeptidic mycotoxin produced by the entomopathogenic fungus Metarhizium anisopliae, exhibits insecticidal activities in a wide range of pests and is known as an innate immunity inhibitor. However, its mechanism of action requires further investigation. In this research, the interactions of DA with the six aminoacyl tRNA synthetases (ARSs) of Bombyx mori, BmAlaRS, BmCysRS, BmMetRS, BmValRS, BmIleRS, and BmGluProRS, were analyzed. The six ARSs were expressed and purified. The BLI (biolayer interferometry) results indicated that DA binds these ARSs with the affinity indices (KD) of 10−4 to 10−5 M. The molecular docking suggested a similar interaction mode of DA with ARSs, whereby DA settled into a pocket through hydrogen bonds with Asn, Arg, His, Lys, and Tyr of ARSs. Furthermore, DA treatments decreased the contents of soluble protein and free amino acids in Bm12 cells, which suggested that DA impedes protein synthesis. Lastly, the ARSs in Bm12 cells were all downregulated by DA stress. This study sheds light on exploring and answering the molecular target of DA against target insects.

Conformation transition kinetics of Bombyx mori silk protein

2007 ◽  
Vol 68 (1) ◽  
pp. 223-231 ◽  
Author(s):  
Xin Chen ◽  
Zhengzhong Shao ◽  
David P. Knight ◽  
Fritz Vollrath
Keyword(s):  
Bombyx Mori ◽  
Silk Protein ◽  
Bombyx Mori Silk ◽  
Kinetics Of ◽  
Conformation Transition

scholarly journals Pattern Comparison of in Vivo Protein Synthesis between Normal and "Kidney" Eggs of Bombyx mori(Lepidoptera:Bombycidae)

1992 ◽  
Vol 27 (1) ◽  
pp. 167-170
Author(s):  
Akira SAITO ◽  
Eiichi KOSEGAWA ◽  
Si Kab NHO ◽  
Katsumi KOGA ◽  
Bungo SAKAGUCHI ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Bombyx Mori ◽  
Pattern Comparison ◽  
In Vivo Protein Synthesis
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