Rutin directly affects stimulatory action of FSH on the ovarian cell

2021 ◽  
Vol 15 ◽  
pp. 100247
Author(s):  
Alexander V. Sirotkin ◽  
Betina Pelleova ◽  
Zuzana Fabova ◽  
Pavol Makovicky ◽  
Saleh Alwasel ◽  
...  
Keyword(s):  
Stimulatory Action ◽  
Ovarian Cell

scholarly journals Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1859 ◽  
Author(s):  
Alexander V. Sirotkin ◽  
Monika Radosová ◽  
Adam Tarko ◽  
Zuzana Fabova ◽  
Iris Martín-García ◽  
...  
Keyword(s):  
Cell Viability ◽  
Copper Nanoparticles ◽  
Plant Extracts ◽  
Trypan Blue ◽  
Stimulatory Action ◽  
Ovarian Cell ◽  
Cell Functions ◽  
Ovarian Granulosa Cells ◽  
17Β Estradiol ◽  
Effect Of Copper

The application of nanoparticles has experienced a vertiginous growth, but their interaction with food and medicinal plants in organisms, especially in the control of reproduction, remains unresolved. We examined the influence of copper nanoparticles supported on titania (CuNPs/TiO2), plant extracts (buckwheat (Fagopyrum esculentum) and vitex (Vitex agnus-castus)), phytochemicals (rutin and apigenin), and their combination with CuNPs/TiO2 on ovarian cell functions, using cultured porcine ovarian granulosa cells. Cell viability, proliferation (PCNA accumulation), apoptosis (accumulation of bax), and hormones release (progesterone, testosterone, and 17β-estradiol) were analyzed by the Trypan blue test, quantitative immunocytochemistry, and ELISA, respectively. CuNPs/TiO2 increased cell viability, proliferation, apoptosis, and testosterone but not progesterone release, and reduced the 17β-estradiol output. Plant extracts and components have similar stimulatory action on ovarian cell functions as CuNPs/TiO2, but abated the majority of the CuNPs/TiO2 effects. This study concludes that (1) CuNPs/TiO2 can directly stimulate ovarian cell functions, promoting ovarian cell proliferation, apoptosis, turnover, viability, and steroid hormones release; (2) the plants buckwheat and vitex, as well as rutin and apigenin, can promote some of these ovarian functions too; and (3) these plant additives mitigate the CuNPs/TiO2’s activity, something that must be considered when applied together.

Quercetin directly inhibits basal ovarian cell functions and their response to the stimulatory action of FSH

2019 ◽  
Vol 860 ◽  
pp. 172560 ◽  
Author(s):  
Alexander V. Sirotkin ◽  
Aneta Štochmaľová ◽  
Richard Alexa ◽  
Attila Kádasi ◽  
Miroslav Bauer ◽  
...  
Keyword(s):  
Stimulatory Action ◽  
Ovarian Cell ◽  
Cell Functions

Optimized culture system to maximize ovarian cell growth and functionality in vitro

2021 ◽  
Author(s):  
Myung Jae Jeon ◽  
Young Sik Choi ◽  
James J. Yoo ◽  
Anthony Atala ◽  
John D. Jackson
Keyword(s):  
Cell Growth ◽  
Culture System ◽  
Ovarian Cell

scholarly journals Stimulatory action of cycloheximide on glucose metabolism in the rat epididymal fat pad

1977 ◽  
Vol 18 (1) ◽  
pp. 93-98
Author(s):  
J A García-Sáinz ◽  
E Piña ◽  
V Chagoya de Sánchez
Keyword(s):  
Glucose Metabolism ◽  
Fat Pad ◽  
Stimulatory Action ◽  
Epididymal Fat

scholarly journals Melatonin administration provokes the activity of dendritic reticular cells in the seminal vesicle of Soay ram during the non-breeding season

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hanan H. Abd-Elhafeez ◽  
A. H. S. Hassan ◽  
Manal T. Hussein
Keyword(s):  
Estrogen Receptor Alpha ◽  
Close Contact ◽  
Secretory Activity ◽  
Treated Group ◽  
Nerve Fibers ◽  
Stimulatory Action ◽  
Transmission Electron ◽  
Melatonin Administration ◽  
S 100 ◽  
Lysosomal System

AbstractDendritic cells (DCs) are innate immune cells which engulf, process and present antigens to the naïve T-lymphocyte cells. However, little is known about the effect of melatonin on the DCs. The present study aimed to investigate the morphology and distribution of the DCs by transmission electron microscopy and Immunohistochemistry after melatonin administration. A total of 8 out of 15 adult ram was randomly selected to receive the melatonin implant and the remaining 7 animals received melatonin free implants. DCs showed positive immunoreactivity for CD117, S-100 protein and CD34. There is an obvious increase in the number of the positive immunoreactive cells to CD3, estrogen receptor alpha and progesterone in the treated groups. The expression of CD56 and MHCII in the DCs was abundant in the treated groups. The ultrastructure study revealed that melatonin exerts a stimulatory effect on the DCs which was associated with increment in the secretory activity of DCs. The secretory activity demarcated by an obvious increase in the number of mitochondria, cisternae of rER and a well-developed Golgi apparatus. The endosomal- lysosomal system was more developed in the treated groups. A rod-shaped Birbeck granule was demonstrated in the cytoplasm of the melatonin treated group. DCs were observed in a close contact to telocytes, T-Lymphocytes, nerve fibers and blood vessels. Taken together, melatonin administration elicits a stimulatory action on the DCs and macrophages through increasing the size, the number and the endosomal compartments which may correlate to increased immunity.

Influence of N-methyl-d-aspartate on the reproductive axis of male Syrian hamsters

1993 ◽  
Vol 137 (2) ◽  
pp. 247-NP ◽  
Author(s):  
H. F. Urbanski ◽  
M. M. Fahy ◽  
P. M. Collins
Keyword(s):  
Hormone Secretion ◽  
Serum Testosterone ◽  
Inhibitory Effect ◽  
Stimulatory Action ◽  
Syrian Hamsters ◽  
Reproductive Axis ◽  
Treatment Paradigm ◽  
Testicular Size ◽  
Lh Releasing Hormone

ABSTRACT The influence of excitatory amino acids (EAAs) on reproductive neuroendocrine function was investigated in adult male Syrian hamsters of the LSH/Ss Lak strain. Before the study, the animals were maintained in a sexually regressed condition, under short days (SD) and subsequently were either transferred to long days (LD) or kept under SD, for a further 4 weeks. In the former group, photostimulation produced a predictable elevation in the hypophysial contents and serum concentrations of FSH and LH. This was accompanied by an increase in testicular size, an elevation in serum testosterone levels and an increase in spermatogenic activity; the SD hamsters remained sexually quiescent throughout the study. In contrast, SD hamsters that were given daily injections of the EAA agonist, N-methyl-d,l-aspartate (NMA: 50 mg/kg body weight, s.c.), showed stimulatory responses that were generally even more pronounced than those shown by the LD group. Surprisingly, an identical NMA treatment paradigm failed to cause a similar activation of the reproductive axis in LD hamsters that were given daily afternoon injections of melatonin (25 μg, s.c), even though the inhibitory effect of this melatonin treatment is generally regarded as being comparable with that produced by exposure to SD. Although EAAs can acutely stimulate the neurocircuitry that controls LH-releasing hormone secretion, the present findings suggest that EAAs might also exert a long-term stimulatory action by acting further upstream in the photoneuroendocrine pathway. Journal of Endocrinology (1993) 137, 247–252

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