Melaleuca quinquenervia essential oil inhibits α-melanocyte-stimulating hormone-induced melanin production and oxidative stress in B16 melanoma cells

Phlorotannins isolated from brown algae, such as Eisena bicyclis, have positive physiological effects, including anti-cancer, anti-inflammatory, and anti-Alzheimer’s disease. Although phlorotannins have been shown to inhibit tyrosinase, an enzyme essential for melanogenesis, their effect on melanogenesis remains unexplored. Thus, we isolated phlorotannins from E. bicyclis and examined their effects on α-melanocyte-stimulating hormone (α-MSH)-induced melanogenesis in murine B16 melanoma cells. Both fucofuroeckol-A (FF-A) and phlorofucofuroeckol-A (PFF-A) suppressed α-MSH-induced melanogenesis. Neither inhibited human tyrosinase (TYR) activity, but both inhibited tyrosinase-related protein-2 activity. FF-A downregulated the expression of microphthalmia-associated transcription factor and TYR, which subsequently suppressed melanin production. These results suggest that phlorotannins could be beneficial as melanin control drugs for hyperpigmentation disorders.

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Resveratrol Inhibits Alpha-Melanocyte-Stimulating Hormone Signaling, Viability, and Invasiveness in Melanoma Cells

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/632121 ◽

2013 ◽

Vol 2013 ◽

pp. 1-8 ◽

Cited By ~ 6

Author(s):

Yu-Jen Chen ◽

Ying-Yin Chen ◽

Yi-Feng Lin ◽

Hsuan-Yun Hu ◽

Hui-Fen Liao

Keyword(s):

Stem Cell ◽

Cancer Stem Cell ◽

Nuclear Translocation ◽

Combined Treatment ◽

Treatment Resistance ◽

Melanoma Cells ◽

B16 Melanoma ◽

Melanocyte Stimulating Hormone ◽

B16 Melanoma Cells ◽

Stimulating Hormone

Melanoma is a malignancy with high potential to invasion and treatment resistance. Theα-melanocyte-stimulating hormone (α-MSH) signal transduction involving Wnt/β-catenin, c-Kit, and microphthalmia-associated transcription factor (MITF), a known pathway to produce melanin, has been demonstrated as one of cancer stem cell characteristics. This study was aimed to examine the effect of resveratrol, an abundant ingredient of grape and medicinal plants, onα-MSH signaling, viability, and invasiveness in melanoma cells. Byα-MSH treatment, the melanin production in B16 melanoma cells was augmented as a validation for activation ofα-MSH signaling. The upregulated expression ofα-MSH signaling-related moleculesβ-catenin, c-Kit, and MITF was suppressed by resveratrol and/or STI571 treatment. Nuclear translocation of MITF, a hallmark ofα-MSH signaling activation, was inhibited by combined treatment of resveratrol and STI571. At effective concentration, resveratrol and/or STI571 inhibited cell viability andα-MSH-activated matrix metalloproteinase- (MMP-)9 expression and invasion capacity of B16 melanoma cells. In conclusion, resveratrol enhances STI571 effect on suppressing theα-MSH signaling, viability, and invasiveness in melanoma cells. It implicates that resveratrol may have potential to modulate the cancer stem cell characteristics of melanoma.

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