Pyrroloquinoline quinone (PQQ) inhibits the expression of tyrosinase mRNA by α-Melanocyte stimulating hormone in murine B16 melanoma cells

Life Sciences ◽  
1995 ◽  
Vol 56 (20) ◽  
pp. 1707-1713 ◽  
Author(s):  
Hiroshi Kosano ◽  
Taku Setogawa ◽  
Ken Kobayashi ◽  
Hideo Nishigori
Keyword(s):  
Pyrroloquinoline Quinone ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Melanocyte Stimulating Hormone ◽  
B16 Melanoma Cells ◽  
Stimulating Hormone

scholarly journals Resveratrol Inhibits Alpha-Melanocyte-Stimulating Hormone Signaling, Viability, and Invasiveness in Melanoma Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Yu-Jen Chen ◽  
Ying-Yin Chen ◽  
Yi-Feng Lin ◽  
Hsuan-Yun Hu ◽  
Hui-Fen Liao
Keyword(s):  
Stem Cell ◽  
Cancer Stem Cell ◽  
Nuclear Translocation ◽  
Combined Treatment ◽  
Treatment Resistance ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Melanocyte Stimulating Hormone ◽  
B16 Melanoma Cells ◽  
Stimulating Hormone

Melanoma is a malignancy with high potential to invasion and treatment resistance. Theα-melanocyte-stimulating hormone (α-MSH) signal transduction involving Wnt/β-catenin, c-Kit, and microphthalmia-associated transcription factor (MITF), a known pathway to produce melanin, has been demonstrated as one of cancer stem cell characteristics. This study was aimed to examine the effect of resveratrol, an abundant ingredient of grape and medicinal plants, onα-MSH signaling, viability, and invasiveness in melanoma cells. Byα-MSH treatment, the melanin production in B16 melanoma cells was augmented as a validation for activation ofα-MSH signaling. The upregulated expression ofα-MSH signaling-related moleculesβ-catenin, c-Kit, and MITF was suppressed by resveratrol and/or STI571 treatment. Nuclear translocation of MITF, a hallmark ofα-MSH signaling activation, was inhibited by combined treatment of resveratrol and STI571. At effective concentration, resveratrol and/or STI571 inhibited cell viability andα-MSH-activated matrix metalloproteinase- (MMP-)9 expression and invasion capacity of B16 melanoma cells. In conclusion, resveratrol enhances STI571 effect on suppressing theα-MSH signaling, viability, and invasiveness in melanoma cells. It implicates that resveratrol may have potential to modulate the cancer stem cell characteristics of melanoma.

Investigation of the Regulation of Pigmentation in α-Melanocyte-Stimulating Hormone Responsive and Unresponsive Cultured B16 Melanoma Cells

1989 ◽  
Vol 2 (3) ◽  
pp. 161-166 ◽  
Author(s):  
SARAH E. HILL ◽  
JAYNE BUFFEY ◽  
ANTHONY J. THODY ◽  
IAN OLIVER ◽  
STANLEY S. BLEEHEN ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
B16 Melanoma ◽  
Melanocyte Stimulating Hormone ◽  
B16 Melanoma Cells ◽  
Stimulating Hormone

Torilin fromTorilis japonicaInhibits Melanin Production in α-Melanocyte Stimulating Hormone-Activated B16 Melanoma Cells

Planta Medica ◽  
2009 ◽  
Vol 75 (14) ◽  
pp. 1505-1508 ◽  
Author(s):  
Cheong-Yong Yun ◽  
Dongchun Kim ◽  
Won-Hee Lee ◽  
Yu Park ◽  
Seung Lee ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
B16 Melanoma ◽  
Melanin Production ◽  
Melanocyte Stimulating Hormone ◽  
B16 Melanoma Cells ◽  
Stimulating Hormone

scholarly journals Effect of Phlorotannins Isolated From Eisenia bicyclis on Melanogenesis in Mouse B16 Melanoma Cells

2021 ◽  
Vol 16 (5) ◽  
pp. 1934578X2110192
Author(s):  
Yuki Ohno ◽  
Shiori Kondo ◽  
Kiho Tajima ◽  
Toshiyuki Shibata ◽  
Tomohiro Itoh
Keyword(s):  
Melanoma Cells ◽  
B16 Melanoma ◽  
Physiological Effects ◽  
Related Protein ◽  
Eisenia Bicyclis ◽  
Melanin Production ◽  
Melanocyte Stimulating Hormone ◽  
B16 Melanoma Cells ◽  
Anti Cancer ◽  
Tyrosinase Related Protein

Phlorotannins isolated from brown algae, such as Eisena bicyclis, have positive physiological effects, including anti-cancer, anti-inflammatory, and anti-Alzheimer’s disease. Although phlorotannins have been shown to inhibit tyrosinase, an enzyme essential for melanogenesis, their effect on melanogenesis remains unexplored. Thus, we isolated phlorotannins from E. bicyclis and examined their effects on α-melanocyte-stimulating hormone (α-MSH)-induced melanogenesis in murine B16 melanoma cells. Both fucofuroeckol-A (FF-A) and phlorofucofuroeckol-A (PFF-A) suppressed α-MSH-induced melanogenesis. Neither inhibited human tyrosinase (TYR) activity, but both inhibited tyrosinase-related protein-2 activity. FF-A downregulated the expression of microphthalmia-associated transcription factor and TYR, which subsequently suppressed melanin production. These results suggest that phlorotannins could be beneficial as melanin control drugs for hyperpigmentation disorders.

scholarly journals Verification of the Functional Antioxidant Activity and Antimelanogenic Properties of Extracts of Poria cocos Mycelium Fermented with Freeze-Dried Plum Powder

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Kyung-Yun Kang ◽  
Yun-Ho Hwang ◽  
Sung-Ju Lee ◽  
Ho-Yeol Jang ◽  
Seong-Gyeol Hong ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Melanoma Cells ◽  
Significant Inhibition ◽  
B16 Melanoma ◽  
Poria Cocos ◽  
Melanocyte Stimulating Hormone ◽  
B16 Melanoma Cells ◽  
Freeze Dried ◽  
Culture Substrate ◽  
Fatigue Recovery

Here we examine the effects of extracts of Poria cocos mycelium fermented with freeze-dried plum powder (PPE) on the α-melanocyte stimulating hormone (α-MSH)-stimulated melanogenesis in cultured murine B16 melanoma cells (B16 cells), relative to the effects of Prunus extract. We found that an extract of Prunus fermentation showed significant inhibition of melanogenesis and tyrosinase activity with no effect on cell proliferation and was more active compared to Prunus extract alone. Furthermore, we confirmed that medium containing 3% Prunus was the optimal culture substrate for fermentation with Poria cocos. These results provide evidence that Prunus fermentation extract affects skin whiting in murine B16 melanoma cells (B16 cells). Prunus contains rutin, oxalic acid, succinic acid, and fumaric acid, which help in digestion and fatigue recovery. The rutin of Prunus mume is reported to have antioxidant and anti-inflammatory effects. Also, Prunus extract has a tyrosinase inhibitory activity for skin whiting through its antioxidant activity. Therefore, we believe the Prunus extract for Poria cocos fermentation can be provided as a potential mediator to induce skin whiting.

scholarly journals Elucidation of Melanogenesis-Associated Signaling Pathways Regulated by Argan Press Cake in B16 Melanoma Cells

Nutrients ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 2697
Author(s):  
Thouria Bourhim ◽  
Myra O. Villareal ◽  
Chemseddoha Gadhi ◽  
Hiroko Isoda
Keyword(s):  
Signaling Pathways ◽  
Press Cake ◽  
Global Gene Expression ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Dependent Manner ◽  
Melanin Biosynthesis ◽  
B16 Melanoma Cells ◽  
Pigmentary Disorders ◽  
Argan Oil

The beneficial effect on health of argan oil is recognized worldwide. We have previously reported that the cake that remains after argan oil extraction (argan press-cake or APC) inhibits melanogenesis in B16 melanoma cells in a time-dependent manner without cytotoxicity. In this study, the global gene expression profile of B16 melanoma cells treated with APC extract was determined in order to gain an understanding of the possible mechanisms of action of APC. The results suggest that APC extract inhibits melanin biosynthesis by down-regulating microphthalmia-associated transcription factor (Mitf) and its downstream signaling pathway through JNK signaling activation, and the inhibition of Wnt/β-catenin and cAMP/PKA signaling pathways. APC extract also prevented the transport of melanosomes by down-regulating Rab27a expression. These results suggest that APC may be an important natural skin whitening product and pharmacological agent used for clinical treatment of pigmentary disorders.

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