Energy transfer by resonant dipole–dipole interaction from a conjugated polymer to a quantum-dot

2005 ◽  
Vol 28 (1) ◽  
pp. 66-75 ◽  
Author(s):  
Suc-Kyoung Hong
2003 ◽  
Vol 773 ◽  
Author(s):  
Aaron R. Clapp ◽  
Igor L. Medintz ◽  
J. Matthew Mauro ◽  
Hedi Mattoussi

AbstractLuminescent CdSe-ZnS core-shell quantum dot (QD) bioconjugates were used as energy donors in fluorescent resonance energy transfer (FRET) binding assays. The QDs were coated with saturating amounts of genetically engineered maltose binding protein (MBP) using a noncovalent immobilization process, and Cy3 organic dyes covalently attached at a specific sequence to MBP were used as energy acceptor molecules. Energy transfer efficiency was measured as a function of the MBP-Cy3/QD molar ratio for two different donor fluorescence emissions (different QD core sizes). Apparent donor-acceptor distances were determined from these FRET studies, and the measured distances are consistent with QD-protein conjugate dimensions previously determined from structural studies.


2006 ◽  
Vol 97 (16) ◽  
Author(s):  
Sebastian Westenhoff ◽  
Wichard J. D. Beenken ◽  
Richard H. Friend ◽  
Neil C. Greenham ◽  
Arkady Yartsev ◽  
...  

Author(s):  
Hsin-Chih Yeh ◽  
Christopher M. Puleo ◽  
Yi-Ping Ho ◽  
Tza-Huei Wang

In this report, we review several single-molecule detection (SMD) methods and newly developed nanocrystal-mediated single-fluorophore strategies for ultrasensitive and specific analysis of genomic sequences. These include techniques, such as quantum dot (QD)-mediated fluorescence resonance energy transfer (FRET) technology and dual-color fluorescence coincidence and colocalization analysis, which allow separation-free detection of low-abundance DNA sequences and mutational analysis of oncogenes. Microfluidic approaches developed for use with single-molecule detection to achieve rapid, low-volume, and quantitative analysis of nucleic acids, such as electrokinetic manipulation of single molecules and confinement of sub-nanoliter samples using microfluidic networks integrated with valves, are also discussed.


2013 ◽  
Vol 8 (1) ◽  
pp. 452 ◽  
Author(s):  
Yulia A Gromova ◽  
Anna O Orlova ◽  
Vladimir G Maslov ◽  
Anatoly V Fedorov ◽  
Alexander V Baranov

2010 ◽  
Vol 18 (24) ◽  
pp. 24486 ◽  
Author(s):  
Clare Higgins ◽  
Manuela Lunz ◽  
A. Louise Bradley ◽  
Valerie A. Gerard ◽  
Stephen Byrne ◽  
...  

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