Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (−)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries

2004 ◽  
Vol 65 (19) ◽  
pp. 2649-2659 ◽  
Author(s):  
Joost Lücker ◽  
Pat Bowen ◽  
Jörg Bohlmann
Keyword(s):  
Vitis Vinifera ◽  
Functional Identification ◽  
Sesquiterpene Synthase ◽  
Germacrene D

scholarly journals Analysis of sesquiterpene hydrocarbons in grape berry exocarp (Vitis vinifera L.) using in vivo-labeling and comprehensive two-dimensional gas chromatography–mass spectrometry (GC×GC–MS)

2019 ◽  
Vol 15 ◽  
pp. 1945-1961 ◽  
Author(s):  
Philipp P Könen ◽  
Matthias Wüst
Keyword(s):  
Gas Chromatography ◽  
Vitis Vinifera ◽  
Solid Phase ◽  
Natural Compounds ◽  
Two Dimensional ◽  
Sesquiterpene Hydrocarbons ◽  
In Vivo Labeling ◽  
Germacrene D ◽  
Tof Ms

Sesquiterpenes are structurally diverse, potent flavoring substances that significantly influence the aroma profile of grapes (Vitis vinifera L.) at the time of physiological ripening. To investigate these natural compounds, freshly harvested, ripe berries of the red wine variety Lemberger (Vitis vinifera subsp. vinifera L.) were analyzed using comprehensive two-dimensional gas chromatography (GC×GC) coupled to a time-of-flight mass spectrometer (TOF–MS) after headspace-solid phase microextraction (HS-SPME). The identification of structurally complex natural compounds, such as sesquiterpenes from fruits and vegetables, is often reported as “tentative”, as authentic standards are not commercially available for most of the analytes. For this reason, feeding experiments (in vivo labeling) were carried out using the stable isotope-labeled precursors [5,5-2H2]-1-deoxy-ᴅ-xylulose (d 2-DOX) and [6,6,6-2H3]-(±)-mevalonolactone (d 3-MVL) to clearly identify the volatiles. Based on the recorded mass spectra of the unlabeled and deuterated compounds, mechanisms for sesquiterpene formation in V. vinifera could be proposed and already known pathways could be confirmed or disproved. For example, the HS-SPME–GC×GC–TOF–MS measurements of fed sample material showed that the tricyclic sesquiterpene hydrocarbons α-copaene, β-copaene, α-cubebene, β-cubebene and the bicyclic δ-cadinene were biosynthesized via (S)-(−)-germacrene D rather than via (R)-(+)-germacrene D as intermediate.

Cloning, functional identification and structural modelling of Vitis vinifera S-adenosylmethionine decarboxylase

2007 ◽  
Vol 164 (9) ◽  
pp. 1208-1219 ◽  
Author(s):  
Annalisa Tassoni ◽  
Marina Franceschetti ◽  
Gianluca Tasco ◽  
Rita Casadio ◽  
Nello Bagni
Keyword(s):  
Vitis Vinifera ◽  
Structural Modelling ◽  
Functional Identification ◽  
Adenosylmethionine Decarboxylase

scholarly journals Improved selectivity of an engineered multi-product terpene synthase

2014 ◽  
Vol 12 (23) ◽  
pp. 4013-4020 ◽  
Author(s):  
Ryan Lauchli ◽  
Julia Pitzer ◽  
Rebekah Z. Kitto ◽  
Karolina Z. Kalbarczyk ◽  
Kersten S. Rabe
Keyword(s):  
Farnesyl Diphosphate ◽  
Terpene Synthase ◽  
Kinetic Properties ◽  
Natural Substrate ◽  
Wild Type ◽  
Random Mutation ◽  
Sesquiterpene Synthase ◽  
Germacrene D

Random mutation of the multi-product sesquiterpene synthase Cop2 generated a mutant that converted the natural substrate farnesyl diphosphate (FPP) into germacrene D-4-ol with 77% selectivity without detrimental effects on wild-type kinetic properties.

Preclinical evaluation of red grapes seeds extract from Vitis vinifera, Burgund Mare, Recas, Romania as skin photochemoprotective agent

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
P Bolfa ◽  
F Sarac ◽  
A Filip ◽  
A Gal ◽  
M Taulescu ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Red Grapes ◽  
Preclinical Evaluation

Resveratrol oligomers from Vitis Vinifera L. stems

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
H Amira-Guebailia ◽  
T Richard ◽  
S Rouaiguia ◽  
P Waffo Tueguo ◽  
JC Delaunay ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Vitis Vinifera L

Biocompatibility of Silver Nano Bio-conjugates from Vitis vinifera Seeds and its Major Component Resveratrol

2016 ◽  
Vol 6 (3) ◽  
Author(s):  
R. Preethi ◽  
P. Padma
Keyword(s):  
Zeta Potential ◽  
Vitis Vinifera ◽  
Silver Ions ◽  
Seed Extract ◽  
Polydispersity Index ◽  
Potential Range ◽  
Visible Spectroscopy ◽  
Grape Seeds ◽  
X Ray ◽  
Uv Visible

The study focused on the green synthesis of silver nanobioconjugates (AgNPs) from phenolic-rich fruit source, Vitis vinifera seed extract and its major component phenolic, resveratrol respectively. Sunlight exposure for 20 minutes was the method of choice for the synthesis of AgNPs of the extract as well as the phenolic, resveratrol. The synthesized nanobioconjugates were characterized using UV-Visible spectroscopy, Transmission electron microscopy (TEM), Energy dispersive X-ray analysis (EDAX), X-ray diffraction (XRD), Polydispersity index, Zeta potential and Fourier transform infrared spectroscopy (FTIR). The reduction of silver ions was confirmed by UV-visible spectroscopy with peaks at 440nm for both nanobioconjugates synthesized from seed extract and compound. The nanobioconjugates showed the spherical in shape with 14-35nm in size and crystalline in nature. The conjugates are well dispersed with 0.301 and 0.287 polydispersity index and the zeta potential range at -13.6 and -14.3mV for stability. The FTRI data proved that the components in grape seeds act as good reductants and stabilizers for the silver nanobioconjugate synthesis. All the synthesized nanobioconjugates exhibited steady and sustained release of the medicinal components conjugated, proving their druggability, and were biocompatible with human cells, demonstrating their safety. The findings of the study validate the anticancer properties of silver nanobioconjugates of Vitis vinifera and its active component resveratrol.

Biotransformation of 4-hydroxybenzoic acid in the rhizosphere of grapevine (Vitis vinifera L.)

2017 ◽  
Vol 40 (1) ◽  
pp. 95-102 ◽  
Author(s):  
B. Wang ◽  
T. Zhou1 ◽  
K. Li ◽  
X.W. Guo ◽  
Y.S. Guo ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Vitis Vinifera L ◽  
Hydroxybenzoic Acid

Isolation and characterization of cell walls from the mesocarp of mature grape berries (Vitis vinifera)

2020 ◽  
Author(s):  
KJ Nunan ◽  
Ian Sims ◽  
A Bacic ◽  
SP Robinson ◽  
GB Fincher
Keyword(s):  
Vitis Vinifera ◽  
Cell Walls ◽  
Vascular Tissue ◽  
Compositional Analysis ◽  
Water Soluble ◽  
Vitis Vinifera L ◽  
Nylon Mesh ◽  
Cytoplasmic Material ◽  
Isolation And Characterization ◽  
Tissue Homogenates

Cell walls have been isolated from the mesocarp of mature grape (Vitis vinifera L.) berries. Tissue homogenates were suspended in 80% (v/v) ethanol to minimise the loss of water-soluble wall components and wet-sieved on nylon mesh to remove cytoplasmic material. The cell wall fragments retained on the sieve were subsequently treated with buffered phenol at pH 7.0, to inactivate any wall-bound enzymes and to dislodge small amounts of cytoplasmic proteins that adhered to the walls. Finally, the wall preparation was washed with chloroform/methanol (1:1, v/v) to remove lipids and dried by solvent exchange. Scanning electron microscopy showed that the wall preparation was essentially free of vascular tissue and adventitious protein of cytoplasmic origin. Compositional analysis showed that the walls consisted of approximately 90% by weight of polysaccharide and less than 10% protein. The protein component of the walls was shown to be rich in arginine and hydroxyproline residues. Cellulose and polygalacturonans were the major constituents, and each accounted for 30-40% by weight of the polysaccharide component of the walls. Substantial varietal differences were observed in the relative abundance of these two polysaccharides. Xyloglucans constituted approximately 10% of the polysaccharide fraction and the remainder was made up of smaller amounts of mannans, heteroxylans, arabinans and galactans.

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