STUDY OF THE INHIBITORY EFFECT OF THE ETHANOLIC EXTRACT OF CORIANDRUM SATIVUM, VITIS VINIFERA, AND ZINGIBER OFFICINALE ON THE GROWTH OF STAPHYLOCOCCUS AUREUS ISOLATED FROM MILK OF COWS INFECTED WITH CLINICAL MASTITIS

2012 ◽  
Vol 11 (2) ◽  
pp. 93-107
Author(s):  
Massar Ibrahim Shekhan ◽  
Jinan Abdul-Amir Sabeeh Al-Hussaini
Keyword(s):  
Staphylococcus Aureus ◽  
Vitis Vinifera ◽  
Ethanolic Extract ◽  
Zingiber Officinale ◽  
Inhibitory Effect ◽  
Clinical Mastitis ◽  
Coriandrum Sativum

scholarly journals Study of the inhibitory effect of Ethanolic extract of( Quercus robur ,Cinnamomum zeylanicum and Thymus vulgaris ) on the growth of Staphylococcus aureus isolated from clinical mastitis in cow

2013 ◽  
Vol 12 (1) ◽  
pp. 96
Author(s):  
Z. A. Hatem
Keyword(s):  
Staphylococcus Aureus ◽  
Quercus Robur ◽  
Culture Media ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Clinical Mastitis ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Cinnamomum Zeylanicum ◽  
Significant Difference

The present study was designed to evaluate the inhibitory effects of three local plant ethanolic extracts (Quercus robur , Cinnamomum zeylanicum and Thymus vulgaris) against the growth of Staphylococcus aureus isolated from milk of cow infected with clinical mastits in culture media by using of agar well diffusion method. For this purpose graduate concentrates for each extract (50 , 100 , 200 , 400 )mg/ml prepared and tested .The result showed that the extract of Quercus robur was more effective followed by Thymus vulgaris and Cinnamomum zeylanicum.The statistical analysis by using ANOVA with LSD at level (p<0.05) showed that there was no significant differences between the effect of the studied concentrations of Quercus robur , Cinnamomum zeylanicum while for Thymus vulgaris we find that the concentration 100 mg\ml had a significant difference with the other studied concentration in inhibition of the growth of the tested bacteria. We also find that between the antibiotics the largest zone of inhibition was given by LOM followed by SPV,PI,NV,RA and CX

scholarly journals Screening of in vitro antimicrobial effects of Helicteres isora extract against Staphylococcus aureus

2021 ◽  
pp. 2313-2316
Author(s):  
Sunisa Sirimongkolvorakul ◽  
Anusorn Jasancheun
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Clinical Mastitis ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Helicteres Isora ◽  
Vitro Model

Background and Aim: Staphylococcus aureus is an important pathogen causing contagious mastitis in cows that need novel treatment rather than antibiotic therapy. This study aimed to investigate the antimicrobial activity of Helicteres isora extracts against S. aureus isolated from subclinical and clinical mastitis cows using an in vitro model. Materials and Methods: H. isora pods were extracted using the following solvents: Distilled water, ethanol, acetone, and methanol. The antimicrobial activity of each extract was determined by the disk diffusion method and broth microdilution assay to assess the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Results: The ethanolic extract of H. isora exhibited the largest inhibition zones against S. aureus (31.05±1.20 mm), followed by the aqueous, methanolic, and acetone extracts, respectively (26.34±1.15, 24.23±0.50, and 22.46±1.53). The ethanolic extract also had a strong inhibitory effect on S. aureus, with MIC and MBC of 0.13 and 0.52 mg/mL, respectively. Conclusion: This study revealed that H. isora is a potential alternative natural antibacterial agent against S. aureus infection. The antimicrobial activity of H. isora is most likely mediated by phytochemical constituents.

scholarly journals INHIBITORY ACTIVITY OF ZINGIBER OFFICINALE VAR RUBRUM EXTRACT AGAINST STAPHYLOCOCCUS AUREUS

2018 ◽  
Vol 1 (3) ◽  
pp. 113
Author(s):  
Firnanda Iptita Dewi ◽  
Manik Retno Wahyunitisari
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Antibacterial Agent ◽  
Inhibitory Concentration ◽  
Zingiber Officinale ◽  
Inhibitory Effect ◽  
Dilution Method ◽  
Ginger Extract ◽  
Minimum Concentration ◽  
Red Ginger

Background: gram-negative bacteria-salmonella typhi and gram-positive bacteria-staphylococcus aureus are types of bacteria that cause infectious disease among several areas in Indonesia. Staphylococcus aureus often found in skin lesions injury. Red ginger extract (Zingiber officinale var rubrum) has been known for its medicinal value especially as an antibacterial agent. Purpose: This study aims to test the ability of Zingiber officinale var rubrum on inhibiting the growth of Staphylococcus aureus. Methods: the activity of the antibacterial agent was calculated based on Minimum Inhibitory Concentration (MIC) using dilution method. Minimum Inhibitory Concentration of extract was determined by incubating bacteria with the extract overnight at 37 0C. Changes in turbidity and inhibition on bacterial growth were then observed. Result: The results show that minimum concentration for inhibitory effect was 1000 μg/mL.Conclusion: extract of Zingiber officinale var rubrum have an inhibitory effect on the growth of Staphylococcus aureus but no activity of killing bacterial was observed on the concentration given.

scholarly journals The Effect of Artichoke Ethanolic Extract on Antibiotic-Resistant Clinical Strains of Staphylococcus aureus Isolated from Women

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Bahman Fazeli-Nasab ◽  
Moharam Valizadeh ◽  
Maryam Beigomi
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Lethal Effect ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant

Objectives: This study aimed to investigate the inhibitory and lethal effect of artichoke on pathogenic strains of Staphylococcus aureus and compare it with antibiotics in vitro. Methods: Ten strains of S. aureus were isolated from the vagina of women in Amir Al-Momenin Hospital of Zabol, Iran. The resistance pattern was determined by the disk diffusion method. Finally, the effect of the extract on bacteria was determined by the 96-well microplate method. Results: The results of the antibiotic resistance pattern showed that S. aureus samples had the highest resistance to oxacillin antibiotic and were sensitive to other antibiotics, and only one sample was sensitive to vancomycin antibiotic. The lowest inhibitory concentration of artichoke against S. aureus was 3.1 mg/mL, but five strains were inhibited at a concentration of 6.25 mg/mL. Conclusions: Ethanolic extract had a significant inhibitory effect on the growth of S. aureus pathogens. Further clinical research is necessary for clinical use of these extracts.

scholarly journals Study of the inhibitory effect of the ethanolic extract of a number of local medicinal plants on the growth of proteus spp. in vitro

2013 ◽  
Vol 37 (1) ◽  
pp. 40-46
Author(s):  
Jinan Abdul-Amir Sabeeh
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Inhibitory Activity ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
The Other ◽  
Coriandrum Sativum ◽  
Thymus Vulgaris

The present study was designed to evaluate the inhibitory effect of six local medicinal plants ethanolic extract ( Quercusrobur, Vitisvinifera, Cinamomumzeylanicum , Ginger officinale ,Thymus vulgaris and Coriandrumsativum ) on the growth of Proteus spp. in vitro. For this purpose graduate concentrates for each extract (50, 100, 200 and 400) mg/ml were prepared and tested. The result showed that all the studied medicinal plants have antibacterial activity against Proteus spp. with exception for Coriandrumsativum which didn’t showed any inhibitory activity for the growing of the studied bacteria in Vitro. And the more active plant in inhibition the growth of the studied bacteria was Quercusrobur followed by Vitisvinifera and Cinamomum zeylanicum , Ginger officinale ,Thymus vulgaris. Also, there was no significant differences between the effect of the different concentrations of Thymus vulgaris and Coriandrum sativum, while there were a significant differences between effect of Cinamomumzeylanicum concentrations and the concentrations of (400 and 200 mg\ml) Ginger officinale and compared with 100 and 50 mg/ml of Quercusrobur, while, the ethanolic extract of the Vitisvinifera showed a significant differences between the effect of the concentration 400 mg\ml with the other studied concentration.

scholarly journals Antibacterial effect of Ginger (Zingiber officinale) against Staphylococcus aureus

Mediscope ◽  
2020 ◽  
Vol 7 (1) ◽  
pp. 31-37
Author(s):  
Md Abdur Rahman ◽  
Shyamal Kumar Shaha ◽  
Syed Didarul Haque ◽  
Refat Zahan ◽  
Tarifat Alam ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Ethanolic Extract ◽  
Zingiber Officinale ◽  
Diffusion Method ◽  
Ginger Extract ◽  
Medical College ◽  
Inhibition Of Growth ◽  
Zones Of Inhibition ◽  
Broth Dilution

This experimental study was carried out to determine the antibacterial effect of Crude Ginger Extract (CGE) and Ethanolic Ginger Extract (EGE) against standard strain of Staphylococcus aureus in the Department of Pharmacology and Therapeutics with the collaboration of Department of Microbiology, Mymensingh Medical College, Mymensingh from July 2016 to June 2017. The growth of Staphylococcus aureus started to be inhibited from 70% CGE incorporated media and complete inhibition of growth occurred at 100% CGE. In case of Ethanolic Extract, sensitivity was seen against Staphylococcus aureus using disc diffusion method. Zones of inhibition were 8, 13 and 19 mm at 25, 50 and 100 μg/10 μl respectively. The minimum inhibitory concentration (MIC) of EGE was assessed by broth dilution technique. The MIC of EGE was 400 μg/ml against Staphylococcus aureus. From the study it is clearly observed that there is definite antibacterial effect of crude ginger extract (CGE) and ethanolic ginger extract against Staphylococcus aureus. Further studies are required to detect and isolate the active ingredients present in the Ginger extract responsible for antibacterial effect. Mediscope Vol. 7, No. 1: Jan 2020, Page 31-37

scholarly journals Effect of ethanolic extract of Clove (Eugenia caryophyllus) on growth of some types of pathogenic bacteria

2009 ◽  
Vol 3 (1) ◽  
pp. 72-78
Author(s):  
Huda Suhail Abid
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Salmonella Typhimurium ◽  
Pathogenic Bacteria ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Clove Extract ◽  
High Inhibitory Effect

Effect of ethanolic extract of Clove (Eugenia caryophyllus) examined against (4) species of pathogenic bacteria ('Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium) using: diffusion method, determination the minimum inhibitory concentration MIC, and minimum bactericidal concentration MBC. The results showed that Clove extract appeared high inhibitory effect on the growth of' 'Staphylococcus aureus & Pseudomonas aeruginosa, this inhibition increased with increasing the concentration. The MIC values were, 2.0, 4.0 mg/ml respectively, on the other hand, the MBC value against 'Staphylococcus aureus was 4.0 mg/ml. while the MBC value against Pseudomonas aeruginosa dose not effective. Further Escherichia coli & Salmonella typhimurium showed resistance to the activity of Clove extract.

scholarly journals Evaluation of Antimicrobial Activity of Several Medicinal Plants Against Clinically Isolated Staphylococcus Aureus from Humans and Sheep

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Bahman Fazeli-Nasab ◽  
Laleh Shahraki-Mojahed ◽  
Gelareh Sohail-beygi ◽  
Mehrangiz Ghafari
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Thymus Vulgaris ◽  
Taxus Baccata ◽  
Growth Inhibition Zone ◽  
Hypericum Perforatum L ◽  
The University

Background: Continuous and indiscriminate use of chemical drugs causes an important phenomenon of resistance to microorganisms. Accordingly, the effect of medications is minimized or offset, increasing drug use and the need to study mixtures with more latest and powerful formulations. On the other hand, it has been reported that many plants essential oils have a significant inhibitory effect on pathogenic microorganisms. Objectives: This study aimed to evaluate the antimicrobial activity of some curative herbs against some clinical bacteria of humans and sheep. Methods: The leaves of chicory (Cichorium intybus L.), Hypericum perforatum L., Lavandula angustifolia, Thymus vulgaris L., and Taxus baccata L. were collected and determined in the botanical laboratory of the University of Zabol. Forty grams of dried leaves was used in 400 mL of ethanol (96%) to prepare the ethanolic extract. DPPH was used to determine the activity of reactive oxygen species (ROS) trapping. The antimicrobial effects were studied by the disk diffusion (6 mm) method in Müller-Hinton agar medium according to the method by Bauer et al. Results: The minimum inhibitory concentrations (MICs) of chicory, thyme, H. perforatum, French lavender, and yarrow extracts in human clinical Staphylococcus aureus were 6.25, 12.5, 3.1, 25, and 6.25 ppm, respectively, but, in sheep, clinical S. aureus, were 12.5, 6.25, 3.1, 12.5, and 25 ppm, respectively. The minimum bactericidal concentrations (MBCs) of chicory, thyme, H. perforatum, French lavender, and yarrow extracts in human clinical S. aureus were 12.5, 25, 6.25, 50, and 12.5 ppm, respectively, but, in sheep clinical S. aureus, were 25, 12.5, 6.25, 25, and 50 ppm, respectively. The most effective extract in inhibiting the growth of S. aureus was the H. perforatum L. extract with an 8.9-mm diameter growth inhibition zone. Conclusions: Regarding the side effects of artificial medications and antimicrobials, as well as the significant influence of healing herb extracts used in this study, it was found that H. perforatum was the most effective plant against S. aureus. It should be noted that plant extracts were more effective in human clinical S. aureus than in sheep clinical S. aureus.

scholarly journals In-vitro antibiofilm activity of selected medicinal plants against Staphylococcus aureus biofilm on chitin flakes as substrate

2020 ◽  
Vol 11 (2) ◽  
pp. 1595-1603
Author(s):  
Rimashree Baishya ◽  
Soma Banerjee
Keyword(s):  
Staphylococcus Aureus ◽  
Plant Extract ◽  
Curcuma Longa ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Dilution Method ◽  
Antibiofilm Activity ◽  
Phytochemical Analysis ◽  
Cell Counts

The aim of the study is to assess the antibiofilm activity of ethanolic extracts of few Indian traditional herbal plants against Staphylococcus aureus biofilm along with phytochemical analysis of plant extracts and identification of active compounds. Broth micro-dilution method was followed for minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) study of the plants against Staphylococcus aureus ATCC 25923. Chitin was used as a substrate for biofilm formation and antibiofilm activity of plant extract was investigated. Scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FT-IR) of the chitin flakes were analysed to determine the inhibitory effect of the plant extract on Staphylococcus aureus biofilm. Curcuma longa showed the best antibacterial activities where MIC and MBC were 0.4 mg/ml and 1.60 mg/ml respectively. Viability cell counts of both planktonic and sessile condition of Staphylococcus aureus showed that C.longa ethanolic extract have an antibiofilm activity. The major compounds of C.longa extract was found to be curcumin, which responded for antibiofilm activity against S. aureus. Curcuma longa rhizome and its active compound curcumin can be a potential natural source of antibiofilm agents against infectious biofilm forming Staphylococcus aureus.

A comparative study of the antibacterial activity of Quercus robur (Ethanolic extract) and Zinc oxide nanoparticles on Salmonella (In vitro)

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Hams H. H. Alfattli ◽  
Ghufran Zuhair Jiber ◽  
Ghaidaa Gatea Abbass
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Quercus Robur ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Oxide Nanoparticles ◽  
Inhibition Zones

This study which designed to evaluate the inhibitory effect of Ethanolic extract of (Quercusrobur) and Zinc oxide nanoparticles on the growth of one genus of enterobacteriacae (Salmonella). In vitro. For this purpose graduate concentrates for plant extract (50, 100, 200, 400 )mg/ml which prepared and compared with Zinc oxide nanoparticles of different concentration (2, 1, 0.5, 0.25) μg/ml,and examined. The result showed that the studied medicinal plant has antibacterial activity against this bacteria which used. The result showed that the plant has good activity in decrease the growth of this bacteria. The results of the study also showed that the nano-ZnO has very effective antibacterial action against the studied bacteria which was Salmonella,nanoparticles concentrations lead to increasing in the inhibition zones of tested bacterial growth. We also study the effect of three antibiotics Lomefloxacin (LOM), Ciprofloxacin (SIP) and Rifampin (RA) and the result showed,in a comparison within the tested bacteria,Salmonella had a significant inhibition increase in Lomefloxacin ; the ciprofloxacin showed effect on tested bacteria. However,Rifampin does not show any effect on tested bacteria.

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