Identification, characterization, and antifungal activity of cysteine peptidases from Calotropis procera latex

2021 ◽

Vol 9 (10) ◽

pp. 875-883

Author(s):

Mubashira Begum

Keyword(s):

Antifungal Activity ◽

Aspergillus Niger ◽

Aspergillus Flavus ◽

Trichoderma Viride ◽

Aloe Vera ◽

Diffusion Method ◽

Calotropis Procera ◽

Calotropis Gigantea ◽

Fungal Strains ◽

Plumeria Rubra

Abstract: Latex is a natural plant polymer and milky white fluid distributed throughout the plant body like leaves, stems, roots and fruits of all flowering plants and consists of proteins, alkaloids, starches, sugars, oils, tannins, resins and gums that coagulate on-air exposure. It is secreted by specialized plant cells called Laticifers. Plants exude latex in response to physical damage. The present study was carried out to assess the potential antifungal activity of latex of five different plants namely Plumeria rubra, Plumeria alba, Aloe vera, Calotropis procera and Calotropis gigantea against three different pathogenic strains of fungi. All five latexes were found to show good to moderated activity against all the three fungal strains, namely Trichoderma viride, Aspergillus flavus and Aspergillus niger. The latex of each plant was tested in two volumes (i.e. 10µl & 20µl), and it was found that the antifungal activity was volume-dependent, and a significant difference was also observed in the case of different fungal strains. The antifungal activity of plant latexes was evaluated by Agar well diffusion method; results revealed that among all the five latexes, the fresh latex of Plumeria rubra (Vol: 10µl &20µl) showed excellent antifungal activity against Aspergillus niger and Aspergillus flavus, whereas fresh latex of Aloe vera (Vol: 10µl & 20µl) showed excellent antifungal activity against Trichoderma viride. The results of the current research imply that the antifungal activity of latex varies with the species of plants and the fungal strains used. The results therefore demonstrated that, the used five latexes effectively inhibited the growth of three tested fungi. Hence, these plant latexes are natural, ecofriendly and can be used as good candidates for the treatment of various fungal diseases. In further words, they can be used for therapy of antifungal-resistant fungi. Keywords: Antifungal activity, Proteins, Alkaloids, Tannins, Resins, Laticifers, Plumeria rubra, Plumeria alba, Aloe vera, Calotropis procera, Calotropis gigantea, Aspergillus flavus, Aspergillus niger, Trichoderma viride, Latex, Agar well diffusion.

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New insights into the complex mixture of latex cysteine peptidases in Calotropis procera

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2013.04.001 ◽

2013 ◽

Vol 58 ◽

pp. 211-219 ◽

Cited By ~ 38

Author(s):

M.V. Ramos ◽

E.S. Araújo ◽

T.L. Jucá ◽

A.C.O. Monteiro-Moreira ◽

I.M. Vasconcelos ◽

...

Keyword(s):

Complex Mixture ◽

Calotropis Procera ◽

Cysteine Peptidases

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Antifungal activity of α-helical propeptide SnuCalCpI15 derived from Calotropis procera R. Br. against food spoilage yeasts

Food Control ◽

10.1016/j.foodcont.2021.108628 ◽

2021 ◽

pp. 108628

Author(s):

Hye-Lin Han ◽

Chang Woo Kwon ◽

Yoonseok Choi ◽

Pahn-Shick Chang

Keyword(s):

Antifungal Activity ◽

Calotropis Procera ◽

Food Spoilage ◽

Spoilage Yeasts ◽

Food Spoilage Yeasts

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Antifungal activity of leaf extract against mycotoxin producing fungi

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i2.2279 ◽

2020 ◽

Vol 11 (2) ◽

pp. 2650-2656

Author(s):

Varsha A Bhalerao ◽

Ashok M Chavan

Keyword(s):

Antifungal Activity ◽

Azadirachta Indica ◽

Disease Transmission ◽

Leaf Extract ◽

Economic Loss ◽

Storage Condition ◽

Aspergillus Ochraceus ◽

Ocimum Sanctum ◽

Calotropis Procera ◽

Datura Metel

About 90% of the world food crops are being produced by using seeds. Healthy seeds are important for production of healthy crop and these seeds are also responsible for disease transmission. It happens either in field or in post-harvest storage condition. Due to these seed borne fungi, seed get deteriorated which may cause a great economic loss as well as several types of abnormalities occur in seeds. Major storage grain fungi contaminated with mycotoxin, mycotoxins contaminants of fungal origin occurring worldwide and characterized by its acute and chronic toxic effects on human health. In the present attempt were made to study the antifungal activity of leaf extract against mycotoxin producing fungi. Fungi isolated from stored seed grains like cereals, pulses, infected oil seeds were collected from from Marathwada region of Maharashtra, India. Antifungal activity of leaf extract of Calotropis procera, Azadirachta indica, Ocimum sanctum, Withania somnifera and Datura metel were tested against Alternaria alternata, Aspergillus flavus, Aspergillus ochraceus, Fusarium gramineorum and Penicillium citrinum by using 96-well plate method. Leaf extract showed remarkable activity against tested fungi at different concentrations as Minimum Inhibitory Concentration. The leaf extract of Calotropis procera and Azadirachta indica, have good potential antifungal properties against different mycotoxin producing fungi.

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